Vitrification: morphological and physiological disorders of in vitro plants

Ziv, Meira

doi:10.1007/978-94-009-2075-0_4

Cited by 248 publications

(178 citation statements)

References 81 publications

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“…In general, the leaves of hyperhydric shoots are thicker, elongated, wrinkled and/or curled, and can be brittle when compared to normal leaves. Anatomical characteristics change in hyperhydric shoots and several types of abnormal structures can be recognized (Ziv 1991). Several factors can induce the hyperhydricity in explants, such as high BAP levels (Oliveira et al 2010), cultivar (Carvalho et al 2013), and agar concentration (Abdoli et al 2007) but the most common cause of the hyperhydricity occurrence is the culture in liquid medium (Scheidt et al 2011;Silva et al 2012).…”

Section: Lavandula Angustifolia Mill (Syn L Officinalismentioning

confidence: 99%

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

Machado

Silva

Biasi

et al. 2014

Braz. arch. biol. technol.

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Section: Lavandula Angustifolia Mill (Syn L Officinalismentioning

confidence: 99%

Influence of calcium content of tissue on hyperhydricity and shoot-tip necrosis of in vitro regenerated shoots of Lavandula angustifolia Mill.

Machado

Silva

Biasi

et al. 2014

Braz. arch. biol. technol.

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“…cultura de tecidos, gerando anormalidades fisiológicas e morfológicas no tecido vegetal, afetando os processos de fotossíntese e trocas gasosas da planta (Ziv, 1991). Um dos fatores que provoca essa anormalidade é o excesso de regulador vegetal.…”

Section: Concentração De Bap (Mg L -1 )unclassified

Multiplicação in vitro e aclimatação de Vernonia condensata Baker

Vicente

Almeida

Carvalho

2009

Rev. bras. plantas med.

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RESUMO:A espécie medicinal Vernonia condensata, vulgarmente conhecida por alumã, pertencente à família Asteraceae, possui propriedades analgésicas e de proteção gástrica. A crescente utilização dessa planta no Nordeste, pelas propriedades terapêuticas, justifica a necessidade de medidas que minimizem o impacto de sua exploração nas reservas naturais. O objetivo desse trabalho foi multiplicar in vitro plantas de alumã sob diferentes concentrações de BAP e aclimatá-las. Gemas axilares foram desinfestadas em solução de álcool etílico 70%, durante 2 minutos e em solução de hipoclorito de sódio (2% de cloro ativo) na concentração de 3:1, durante 15 minutos, seguido de três lavagens em água destilada estéril. Os tratamentos para multiplicação consistiram em doses de BAP (0,0; 1,0; 2,0; 3,0; 4,0 e 5,0 mg L -1 ) em meio MS semi-sólido. O delineamento experimental foi o inteiramente casualizado, com 5 repetições, contendo 10 gemas por repetição. Após 30 dias de cultivo observou-se maior taxa de explantes responsivos, 84% na concentração de 1,0 mg L -1 de BAP, com produção de 4,0 brotos/explante. Nos tratamentos 3,0; 4,0 e 5,0 mg L -1 ocorreu hiperhidricidade nas folhas. As microplantas de alumã provenientes da metodologia utilizada neste trabalho alcançaram 100% de sobrevivência na aclimatação. Palavras-chave: biotecnologia, plantas medicinais, cultura de tecidos ABSTRACT:In vitro multiplication and acclimation of Vernonia condensata Baker. The medicinal species Vernonia condensata, commonly known as "alumã", belongs to the family Asteraceae and has analgesic and gastric protective properties. The increasing use of this plant in the Northeast of Brazil due to its therapeutic properties justifies the need of measures to minimize the impact of its exploitation in natural reserves. The aim of this study was to multiply, in vitro, "alumã" plants under different BAP levels, acclimating them. Axillary buds were sterilized in 70% (v/v) alcohol solution for 2 minutes and in 75% sodium hypochlorite solution (2% active chlorine) at 3:1 concentration for 15 minutes, followed by three washings in sterile distilled water. Multiplication treatments consisted of different BAP levels (0.0; 1.0; 2.0; 3.0; 4.0 and 5.0 mg L -1 ) in semi-solid MS medium. The experimental design was completely randomized, with 5 replicates and 10 buds per replicate. After 30 days of cultivation, the highest rate of responsive explants was obtained: 84% at 1.0 mg L -1 BAP, producing 4.0 sprouts/explant. In the treatments 3.0, 4.0 and 5.0 mg L -1 , there were vitrified leaves. The "alumã" microplants used in this study had 100% survival in acclimation.

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“…The phenomenon has been considered as a morpho-physiology disorder and many physiological and biochemical changes have been observed (Ziv, 1991;Franck et al, 2004). Some of these factors include the presence in the medium of growth regulators, of large quantities of NH4 + and Cl -ions, the type and concentration of the gelling agent, high relative humidity in the culture vessels (Debergh et al, 1983;Ziv, 1991;Kevers et al, 2004;Hazarika, 2006) and the successive subcultures of the explants in a culture medium with cytokinin (Vieitez et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

“…Gelling agents are not an ''inert'' medium component, and their type and concentration have a significant effect on the performance of tissue cultured plant material (Debergh, 1983;Ziv, 1991;Pereira-Netto et al, 2007), including the occurrence of hyperhydricity .…”

Section: Introductionmentioning

confidence: 99%