Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering

Mallis, Panagiotis; Katsimpoulas, Michalis; Kostakis, Alkiviadis; Dipresa, Daniele; Korossis, Sotirios; Papapanagiotou, Angeliki; Kassi, Eva; Stavropoulos-Giokas, Catherine; Michalopoulos, Efstathios

doi:10.1007/s13770-020-00243-x

Cited by 23 publications

(26 citation statements)

References 21 publications

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“…Typically, the DNA samples can be loaded onto 1–2% w / v agarose gels and observed under UV light. The absence of dense DNA bands or bands with less than 200 bp DNA confirms further the successful decellularization [ 172 ].…”

Section: Decellularized Vascular Graftsmentioning

confidence: 86%

“…However, the PicoGreen assay can detect only the ds DNA, while the ss DNA cannot be quantified. On the other hand, the spectrophotometric quantification of DNA by measuring the ratio of absorbance 260 nm/280 nm, may provide more data regarding the presence of the total DNA in the acellular scaffold [ 172 ]. It is known that either ss or ds DNA can induce the host’s immune reaction, and the accurate DNA quantification is of major importance.…”

Section: Decellularized Vascular Graftsmentioning

confidence: 99%

“…It is known that either ss or ds DNA can induce the host’s immune reaction, and the accurate DNA quantification is of major importance. Furthermore, the remaining DNA in the scaffolds can be evaluated through the performance of gel electrophoresis [ 172 ]. Typically, the DNA samples can be loaded onto 1–2% w / v agarose gels and observed under UV light.…”

Section: Decellularized Vascular Graftsmentioning

confidence: 99%

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Future Perspectives in Small-Diameter Vascular Graft Engineering

et al. 2020

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The increased demands of small-diameter vascular grafts (SDVGs) globally has forced the scientific society to explore alternative strategies utilizing the tissue engineering approaches. Cardiovascular disease (CVD) comprises one of the most lethal groups of non-communicable disorders worldwide. It has been estimated that in Europe, the healthcare cost for the administration of CVD is more than 169 billion €. Common manifestations involve the narrowing or occlusion of blood vessels. The replacement of damaged vessels with autologous grafts represents one of the applied therapeutic approaches in CVD. However, significant drawbacks are accompanying the above procedure; therefore, the exploration of alternative vessel sources must be performed. Engineered SDVGs can be produced through the utilization of non-degradable/degradable and naturally derived materials. Decellularized vessels represent also an alternative valuable source for the development of SDVGs. In this review, a great number of SDVG engineering approaches will be highlighted. Importantly, the state-of-the-art methodologies, which are currently employed, will be comprehensively presented. A discussion summarizing the key marks and the future perspectives of SDVG engineering will be included in this review. Taking into consideration the increased number of patients with CVD, SDVG engineering may assist significantly in cardiovascular reconstructive surgery and, therefore, the overall improvement of patients’ life.

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Section: Decellularized Vascular Graftsmentioning

confidence: 86%

Section: Decellularized Vascular Graftsmentioning

confidence: 99%

Section: Decellularized Vascular Graftsmentioning

confidence: 99%

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Future Perspectives in Small-Diameter Vascular Graft Engineering

et al. 2020

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“…HUAs are characterized by three distinct layers, tunica intima, media, and adventitia. Endothelial cells (ECs), vascular smooth muscle cells (VSMCs), and perivascular cells (PCs) are located in the tunica intima, media, and adventitia of hUAs, respectively, contributing to various vessels functions [ 15 ]. ECs and VSMCs are responsible for vasoconstriction and vasodilation, through eNOs activation, cyclic GMP accumulation, and phosphorylation of protein kinase A (PKA) [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…In the past, the use of a human umbilical vein in peripheral bypass surgeries, was also evaluated, but due to significant drawbacks such as difficulties in handling, lack of elasticity, increased fragility, and poor patency rates, soon its application was stopped [ 23 ]. Additionally, the development of tissue-engineered vascular grafts from decellularized hUAs, have also been proposed [ 4 , 15 , 17 , 22 , 24 ]. However, in most of these studies, time consuming decellularization protocols, which can last over a week, have been applied to hUAs [ 17 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery

et al. 2020

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The gold standard vascular substitutes, used in cardiovascular surgery, are the Dacron or expanded polytetrafluoroethylene (ePTFE)-derived grafts. However, major adverse reactions accompany their use. For this purpose, decellularized human umbilical arteries (hUAs) may be proven as a significant source for the development of small diameter conduits. The aim of this study was the evaluation of a decellularization protocol in hUAs. To study the effect of the decellularization to the hUAs, histological analysis was performed. Then, native and decellularized hUAs were biochemically and biomechanically evaluated. Finally, broad proteomic analysis was applied. Histological analysis revealed the successful decellularization of the hUAs. Furthermore, a great amount of DNA was removed from the decellularized hUAs. Biomechanical analysis revealed statistically significant differences in longitudinal direction only in maximum stress (p < 0.013) and strain (p < 0.001). On the contrary, all parameters tested for circumferential direction exhibited significant differences (p < 0.05). Proteomic analysis showed the preservation of the extracellular matrix and cytoskeletal proteins in both groups. Proteomic data are available via ProteomeXchange with identifier PXD020187. The above results indicated that hUAs were efficiently decellularized. The tissue function properties of these conduits were well retained, making them ideal candidates for the development of small diameter vascular grafts.

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A Clinical‐Grade Partially Decellularized Matrix for Tracheal Replacement: Validation In Vitro and In Vivo in a Porcine Model

Arakelian,

Léger,

Kellouche

et al. 2024

Advanced Biology

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The management of extensive tracheal resection followed by circumferential replacement remains a surgical challenge. Numerous techniques are proposed with mixed results. Partial decellularization of the trachea with the removal of the mucosal and submucosal cells is a promising method, reducing immunogenicity while preserving the biomechanical properties of the final matrix. Despite many research protocols and proofs of concept, no standardized clinical grade protocol is described. Furthermore, local and systemic biointegration mechanisms of decellularized trachea are not well known. Therefore, in a translational research perspective, this work set up a partial tracheal decellularization protocol in line with Cell and Tissue Products regulations. Extensive characterization of the final product is performed in vitro and in vivo. The results show that the Partially Decellularized Trachea (PDT) is cell‐free in the mucosa and submucosa, while the cartilage structure is preserved, maintaining the biomechanical properties of the trachea. When implanted in the muscle in vivo for 28 days, no systemic inflammation is observed, and locally, the PDT shows an excellent biointegration and vascularization. No signs of graft rejection are observed. These encouraging results confirmed the efficacy of the clinical grade PDT production protocol, which is an important step for future clinical applications.

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Vitrified Human Umbilical Arteries as Potential Grafts for Vascular Tissue Engineering

Cited by 23 publications

References 21 publications

Future Perspectives in Small-Diameter Vascular Graft Engineering

Future Perspectives in Small-Diameter Vascular Graft Engineering

Insights into Biomechanical and Proteomic Characteristics of Small Diameter Vascular Grafts Utilizing the Human Umbilical Artery

A Clinical‐Grade Partially Decellularized Matrix for Tracheal Replacement: Validation In Vitro and In Vivo in a Porcine Model

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