VLDL apoprotein secretion and apo‐B mRNA level in primary culture of cholesterol‐loaded rabbit hepatocytes

Abstract

Incubation of cultured rabbit hepatocytes with p very low density lipoproteins (B-VLDL) induces a dose-dependent increase in cell cholesterol (CH) content and VLDL apoprotein (apo) B and E secretion without change in apo-B mRNA level. These data suggest that j?-VLDL may exert a stimulatory effect on hepatic apo-B production at the co-translational and/or posttranslational level.p-VLDL; Cholesterol; VLDL secretion; Apolipoprotein B; mRNA; (Rabbit hepatocyte)

Post-translational events in the intracellular transit of apolipoprotein-B: modulation by dietary lipids

“…Under conditions in which apo-B secretion by hepatocytes varies up to sevenfold there is no significant change in the mRNA for apo-B (Kosykh et al 1988;Pullinger et al 1989). A number of studies on isolated hepatocytes and hepatoma cells have shown that apo-B is synthesized in excess of that secreted, and that the excess protein is degraded intracellularly (Boren et al 1990(Boren et al , 1993Sato et al 1990;Dixon et al 1991;White et al 1992;Cartwright et al 1993;Dixon & Ginsberg, 1993;Cartwright & Higgins, 1996).…”