Vocal Fold Wound Healing: A Review for Clinicians

Branski, Ryan C.; Verdolini, Katherine; Sandulache, Vlad C.; Rosen, Clark A.; Hebda, Patricia A.

doi:10.1016/j.jvoice.2005.08.005

Cited by 117 publications

(94 citation statements)

References 66 publications

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“…They play critical roles in vocal fold wound healing by maintaining a proper tissue viscoelasticity, assisting collagen assembly, and inhibiting fibrosis or preventing scar formation. 9,55 We discovered that HA and DCN biosynthesis is sensitive to vibrations. Both HAS1 and HAS3 expression were elevated after 7 days of vibrations, and this was confirmed by the HA ELISA results showing an increased HA production for the dynamic cultures, with the OF mode being more effective.…”

Section: Discussionmentioning

confidence: 99%

“…[5][6][7][8] Numerous conditions, such as voice abuse, allergies, intubation, or surgeries, can disrupt the vibratory structures of the LP and compromise the tissue pliability, giving rise to vocal fold dysfunction that affects an estimated 3%-9% of the population. 9 Current treatments for vocal fold disorders usually involve voice therapy, repetitive therapeutic injections, or surgical procedures. 10 These approaches may be effective in improving voice quality temporarily.…”

Section: Introductionmentioning

confidence: 99%

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Modulating the Behaviors of Mesenchymal Stem Cells Via the Combination of High-Frequency Vibratory Stimulations and Fibrous Scaffolds

Tong

Duncan²,

Jia³

2013

Tissue Engineering Part A

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We are interested in the in vitro engineering of artificial vocal fold tissues via the strategic combination of multipotent mesenchymal stem cells (MSCs), physiologically relevant mechanical stimulations, and biomimetic artificial matrices. We have constructed a vocal fold bioreactor that is capable of imposing vibratory stimulations on the cultured cells at human phonation frequencies. Separately, fibrous poly (e-caprolactone) (PCL) scaffolds emulating the ligamentous structure of the vocal fold were prepared by electrospinning, were incorporated in the vocal fold bioreactor, and were driven into a wave-like motion in an axisymmetrical fashion by the oscillating air. MSC-laden PCL scaffolds were subjected to vibrations at 200 Hz with a normal center displacement of *40 mm for a total of 7 days. A continuous (CT) or a 1 h-on-1 h-off (OF) regime with a total dynamic culture time of 12 h per day was applied. The dynamic loading did not cause any physiological trauma to the cells. Immunohistotochemical staining revealed the reinforcement of the actin filament and the enhancement of a 5 b 1 integrin expression under selected dynamic culture conditions. Cellular expression of essential vocal fold extracellular matrix components, such as elastin, hyaluronic acid, and matrix metalloproteinase-1, was significantly elevated as compared with the static controls, and the OF regime is more conducive to matrix production than the CT vibration mode. Analyses of genes of typical fibroblast hallmarks (tenascin-C, collagen III, and procollagen I) as well as markers for MSC differentiation into nonfibroblastic lineages confirmed MSCs' adaptation of fibroblastic behaviors. Overall, the high-frequency vibratory stimulation, when combined with a synthetic fibrous scaffold, serves as a potent modulator of MSC functions. The novel bioreactor system presented here, as a versatile, yet well-controlled model, offers an in vitro platform for understanding vibration-induced mechanotransduction and for engineering of functional vocal fold tissues.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Modulating the Behaviors of Mesenchymal Stem Cells Via the Combination of High-Frequency Vibratory Stimulations and Fibrous Scaffolds

Tong

Duncan²,

Jia³

2013

Tissue Engineering Part A

View full text Add to dashboard Cite

show abstract

“…41 Enriched in the ILP of the vocal fold, HA not only contributes to the maintenance of tissue viscoelasticity but also plays an important role in the wound healing of tissue. 42,43 The 3-day vibratory stimulation resulted in an increase in HA production. However, by day 6, the modulatory effects were abolished irrespective of the culture history or the media composition (data not shown).…”

Section: Vibration and Growth Factor Modulate Stem Cell Behaviorsmentioning

confidence: 97%

Dynamic Vibration Cooperates with Connective Tissue Growth Factor to Modulate Stem Cell Behaviors

Tong

Zerdoum

Duncan

et al. 2014

Tissue Engineering Part A

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“…Extensive work has focused on improving wound closure methods to minimize scar tissue formation, ranging from micro-suturing which allows for primary healing (Woo 1995;Tsuji, Nita et al 2009), to the use of tissue adhesives like fibrin glue (Bleach, Milford et al 1997;Flock 2005;Kitahara, Masuda et al 2005;Finck, Harmegnies et al 2010;Skodacek, Arnold et al 2011), and the use of chemical agents (Campagnolo, Tsuji et al 2010) like Mitomycin-C (Branski, Verdolini et al 2006;Fonseca, Malafaia et al 2010) or stem cells (Hong, Lee et al) to enhance the healing process of vocal fold wounds. However, various challenges faced in the execution of voice microsurgery add to the complexity of wound closure.…”

Section: Introductionmentioning

confidence: 99%