For speech separation method, getting the accurate weight vector is very important for the performance of method. Aiming at how to reduce the signal cancellation, this paper focuses on obtaining the weight vector in the speech separation process accurately, and it designs an adaptive speech separation method based on spherical-regular-tetrahedralmicrophone array. The simulation results show that this paper's method can reduce the signal cancellation effectively in speech separation process, so as to have a better separation performance than the similar method base on two-microphone.