Volatile Acids

Scarisbrick, R.

doi:10.1007/978-3-642-64955-4_14

Cited by 5 publications

(1 citation statement)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analytical methods.-The procedures followed in the quantitative determination of various chemical entities were as follows: for nitrogen, the micro-Kjeldahl method; for acetyl, hydrolysis according to Tracey (1955) and estimation as described by Scarisbrick (1955); for reducing sugars as glucose equivalents, the copper reagent of Somogyi (1952) with the arsenomolybdate reagent of Nelson (1944); for hexosamine, the Immers and Vasseur (1950) modification of the Elson and Morgan (1933) procedure as described by Tracey (1955); for acetyl hexosamine, the method of Reissig et al (1955) ; for protein, the spectrophotometric method of Lowry et al (19511; for glucan, the anthrone procedure described by Ashwell (1957); and for uronic acid, the carbazole method described by Dische (1955). Ash was determined by first heating the sample in a weighed platinum crucible over a Bunsen burner at dull red heat until fuming had ceased and then heating it for 12 hr.…”

mentioning

confidence: 99%

The Chemical Composition of the Hyphal Walls of the Fungus Allomyces

Aronson

Machlis

1959

American J of Botany

View full text Add to dashboard Cite

Aronson, Jerome M., and Leonard Machlis. (U. California, Berkeley.) The chemical composition of the hyphal walls of the fungus Allomyees. Amer. Jour. Bot. 46(4): 292–300. Illus. 1959.—The hyphal walls of Allomyces macrogynus were isloated by both alkaline digestion methods and by sonic oscillation. Both types of preparations showed the walls to consist of chitin, glucan, and ash. In addition, the mechanically isolated walls contained a protein fraction, the properties and significance of which were not determined. Hemicellulose‐type polysaccharides, pectic substances, ether soluble lipids, and constituents giving rise to 3–0‐α‐earboxyethyl hexosamine were not found to be present in the walls. The walls of plants grown for 60–70 hr. under the prescribed conditions contain approximately 60% chitin, 15% glucan, 10% ash, and 10% protein intimately associated with the walls. The percentage of wall material in a mycelium, as well as the percentage of chitin in the walls, increases with the chronological age of the mycelium. These percentages were not, however, affected by variations in the composition of the nutrient medium. The chitin in the walls could be hydrolyzed in the presence of chitinase; lysozyme, however, had no detectable effect on the walls.

show abstract

mentioning

confidence: 99%