Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration

Mira, Sara; Hill, Lisa M.; González‐Benito, M. Elena; Ibáñez, Miguel; Walters, Christina

doi:10.1093/jxb/erv568

Cited by 45 publications

(66 citation statements)

References 44 publications

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“…The mitochondrial membrane is known to be among the primary sites of deterioration, for instance, during seed ageing, because mitochondrial activity has been clearly demonstrated to be the primary source of energy (adenosine triphosphate) for cells (Delouche and Baskin, 1973;Nelson and Cox, 2012). A decrease in the mitochondrial efficiency is correlated with an increase in the production of volatile compounds, especially ethanol (Akimoto et al, 2004;Colville et al, 2012;Mira et al, 2016;Woodstock and Taylorson, 1981). In the past, the method has been based on benchtop multiparameter instruments, using gas chromatography or enzyme sensor technology, both of which are slow and costly for routine analyses of multiple samples (Bicanic et al, 2003;Kataki and Taylor, 2001;Mira et al, 2010;Mira et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…A decrease in the mitochondrial efficiency is correlated with an increase in the production of volatile compounds, especially ethanol (Akimoto et al, 2004;Colville et al, 2012;Mira et al, 2016;Woodstock and Taylorson, 1981). In the past, the method has been based on benchtop multiparameter instruments, using gas chromatography or enzyme sensor technology, both of which are slow and costly for routine analyses of multiple samples (Bicanic et al, 2003;Kataki and Taylor, 2001;Mira et al, 2010;Mira et al, 2016). reported a simplified method of measuring seedproduced ethanol under anaerobic conditions with a modified breath analyser, a so-called fast ethanol assay.…”

Section: Resultsmentioning

confidence: 99%

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Optimization and sensitivity analysis of fast ethanol assay in maize seeds

et al. 2019

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The evaluation of seed deterioration is very important to control the quality of the seeds stored. This study aimed to investigate the potential of fast ethanol assay for seed quality assessment of maize stored under different conditions. The first experiment was to determine the incubating temperature, incubating time, and amount of seed used in the assay. The results showed that the best protocol for the detection of headspace ethanol was incubation of 3 g of maize seed with 20% moisture content (wet basis) in a 20 mL gas chromatography vial at 70 °C for 1.5 h. The assay induced approximately 200-700 µg.L-1 of headspace ethanol, which was sufficient to identify seeds with different vigour levels. In the second experiment, the optimal conditions were used for quality assessment in aged maize seed stored for 12 months under different storage conditions. The increase in the ethanol production of stored maize seed under the controlled conditions (15 °C and 20% RH in the hermetic seal) was lower than under ambient conditions. The ethanol production levels of maize seed samples at the start of storage was significantly lesser than at six months storage (p < 0.05). The test limitations in deteriorated seed with different cultivars and ages will be discussed.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Optimization and sensitivity analysis of fast ethanol assay in maize seeds

et al. 2019

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“…Time for germination percentage to decrease to 80, 50, and 10% (P80, P50, and P10, respectively) was calculated using the dose.p function available in R ( R Core Team, 2016 ). We used the value of P80 to indicate the duration of the initial asymptomatic phase of seed deterioration ( Mira et al , 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Decline in RNA integrity of dry-stored soybean seeds correlates with loss of germination potential

Fleming¹,

Richards²,

Walters³

2017

Journal of Experimental Botany

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110

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“…This process involves several controlling mechanisms associated to progressive molecular damages. Some reactions may occur even when the seeds have low moisture contents, among which fermentation, oxidation, peroxidation and glycation are most recurrent (Mira et al, 2016;Walters et al, 2010). The results of the adjusted emergence curve of S. schizophylla made evident different responses to the temperatures used ( Figure 3).…”

Section: Resultsmentioning

confidence: 99%

Morphological characterization and germination of Syagrus schizophylla (Mart.) Glass. (ARECACEAE)

Beltrame¹,

Jasmim²,

Vieira³

2019

Com. Sci.

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Article AbstractThe interest in Syagrus schizophylla as an ornamental palm tree and the demand for conservation and preservation of the species led to this research. The objective was to study the physiological characteristics of its germination at different temperatures, as well as the morphological and biometrical characterization of diaspores and seedlings at the initial stages of growth and development. The research was divided into two experiments. In the first one, the aim was to identify the water absorption phases of seeds during germination under five scarification treatments as follows: intact diaspores, scarified diaspores, diaspores with endocarp rupture and intact seeds. In the second experiment, germination was tested at 25, 30 e 25 -35 ºC; the first germination count, seedling emergence, abnormal seedlings, non-germinated seeds, the emergence curve, the emergence speed index and the mean time of emergence were evaluated. Afterwards, the morphological and biometrical characteristics of diaspores and seedlings were described. The water absorption curve observed under the different scarification treatments showed different water absorption patterns. Emergence percentages were 53, 61 and 47% at 25, 30 and 25 -35 ºC, respectively. The highest emergence speed index was obtained at 30 ºC. The mean time of emergence was 30 days, approximately, under all the temperatures tested. The diaspores showed a great variability in both shape and size, presenting a globular to ovoid shape with an average length of 2.44 cm and an average width of 1.39 cm. The germination can be classified as hypogeal crypto cotyledonal of the remote-tubular type.

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Volatile emission in dry seeds as a way to probe chemical reactions during initial asymptomatic deterioration

Abstract: HighlightSpecies, storage products, and moisture have large effects on the nature and quantity of volatile emission from dry seeds, but storage time and seed viability do not.

Cited by 45 publications

References 44 publications

Optimization and sensitivity analysis of fast ethanol assay in maize seeds

Optimization and sensitivity analysis of fast ethanol assay in maize seeds

Decline in RNA integrity of dry-stored soybean seeds correlates with loss of germination potential

Morphological characterization and germination of Syagrus schizophylla (Mart.) Glass. (ARECACEAE)

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