Volatile organic compounds and rapid proliferation of Candida pseudolambica W16 are modes of action against gray mold in peach fruit

Zou, Xiurong; Wei, Yingying; Jiang, Shu; Cao, Zidan; Xu, Feng; Wang, Hongfei; Zhan, Pingping; Shao, Xingfeng

doi:10.1016/j.postharvbio.2021.111751

Cited by 29 publications

(12 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Production of VOCs by antagonistic yeasts has been identified as a potential mode of action against a number of pathogens including B. cinerea , Colletotrichum acutatum and various Penicillium species such as P. italicum , P. digitatum and P. expansum under both in vitro and in vivo conditions. Compounds such as phenylethyl alcohol, n-butanol, isoamyl alcohol and 2-methyl-1-propanol have been shown to supress conidia germination and mycelium growth ( di Francesco et al, 2016 ; Yalage Don et al, 2020 ; Zou et al, 2022 ). Furthermore, a compound like dimethyltrisulfide which is not frequently reported in yeast VOCs has been shown to suppress the expression of genes involved in the biosynthesis of β-1,3- d -glucan and chitin in another fruit rotting fungus, Colletrichum gloeosporioides ( Tang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Antifungal activity of non-conventional yeasts against Botrytis cinerea and non-Botrytis grape bunch rot fungi

et al. 2022

View full text Add to dashboard Cite

Grapes harbour a plethora of non-conventional yeast species. Over the past two decades, several of the species have been extensively characterised and their contribution to wine quality is better understood. Beyond fermentation, some of the species have been investigated for their potential as alternative biological tools to reduce grape and wine spoilage. However, such studies remain limited to a few genera. This work aimed to evaluate the antagonistic activity of grape must-derived non-conventional yeasts against Botrytis cinerea and non-Botrytis bunch-rotting moulds and to further elucidate mechanisms conferring antifungal activity. A total of 31 yeast strains representing 21 species were screened on different agar media using a dual culture technique and liquid mixed cultures, respectively. Pichia kudriavzevii was the most potent with a minimum inhibitory concentration of 102 cells/mL against B. cinerea but it had a narrow activity spectrum. Twelve of the yeast strains displayed broad antagonistic activity, inhibiting three strains of B. cinerea (B05. 10, IWBT FF1 and IWBT FF2), a strain of Aspergillus niger and Alternaria alternata. Production of chitinases and glucanases in the presence of B. cinerea was a common feature in most of the antagonists. Volatile and non-volatile compounds produced by antagonistic yeast strains in the presence of B. cinerea were analysed and identified using gas and liquid chromatography mass spectrometry, respectively. The volatile compounds identified belonged mainly to higher alcohols, esters, organosulfur compounds and monoterpenes while the non-volatile compounds were cyclic peptides and diketopiperazine. To our knowledge, this is the first report to demonstrate inhibitory effect of the non-volatile compounds produced by various yeast species.

show abstract

Section: Discussionmentioning

confidence: 99%

Antifungal activity of non-conventional yeasts against Botrytis cinerea and non-Botrytis grape bunch rot fungi

et al. 2022

View full text Add to dashboard Cite

show abstract

“…2-Phenylethanol (2-PE), also called phenylethyl alcohol, exists in a variety of plants (e.g., roses, jasmine, hyacinths, and lilies) and many microorganisms (e.g., Saccharomyces cerevisiae , Kluyveromyces lactis , Kluyveromyces marxianus , Candida pseudolambica , Aureobasidium pullulans , Pichia spp. ). − It is a colorless volatile with a pleasant rose aroma that is listed as Generally Recognized as Safe (GRAS) and has been widely used in cosmetics, fragrance, and food industries. It has antimicrobial activity toward bacteria including Bacillus subtilis , Ralstonia solanacearum , Escherichia coli , Staphylococcus aureus , and Enterococcus faecalis.…”

Section: Introductionmentioning

confidence: 99%

ROS Stress and Cell Membrane Disruption are the Main Antifungal Mechanisms of 2-Phenylethanol against Botrytis cinerea

Zou

Wei

Jiang

et al. 2022

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

2-Phenylethanol (2-PE), a common compound found in plants and microorganisms, exhibits broad-spectrum antifungal activity. Using Botrytis cinerea, we demonstrated that 2-PE suppressed mycelium growth in vitro and in strawberry fruit and reduced natural disease without adverse effects to fruit quality. 2-PE caused structural damage to mycelia, as shown by scanning and transmission electron microscopy. From RNA sequencing analysis we found significantly upregulated genes for enzymatic and nonenzymatic reactive oxygen species (ROS) scavenging systems including sulfur metabolism and glutathione metabolism, indicating that ROS stress was induced by 2-PE. This was consistent with results from assays demonstrating an increase ROS and hydrogen peroxide levels, antioxidant enzyme activities, and malondialdehyde content in treated cells. The upregulation of ATP-binding cassette transporter genes, the downregulation of major facilitator superfamily transporters genes, and the downregulation of ergosterol biosynthesis genes indicated a severe disruption of cell membrane structure and function. This was consistent with results from assays demonstrating compromised membrane integrity and lipid peroxidation. To summarize, 2-PE exposure suppressed B. cinerea growth through ROS stress and cell membrane disruption.

show abstract

“…In accordance with the method of Zou et al (2022) [10], strawberries were wounded as described in 2.3 and then inoculated with S. spartinae W9 or 0.1% β-glucan-treated S. spartinae W9. All strawberries were stored at 20 • C with 90% relative humidity.…”

Section: Population Of Yeasts In Strawberry Woundsmentioning

confidence: 99%

“…Antagonistic yeasts are widely used to control postharvest diseases because of their biosafety, well-defined mechanism of action, and good commercial potential [4][5][6]. A variety of antagonistic yeasts have been reported to control gray mold decay of fruits, including Pichia guilliermondii [7], Wickerhamomyces anomalus [8], Metschnikowia pulcherrima [9], Candida pseudolambica [10], Metarhizium anisopliae [11], Scheffersomyeces spartinae [12], and so on. However, antagonistic yeasts have not been able to achieve the effect of traditional chemical fungicides in controlling postharvest diseases of fruit [13].…”

Section: Introductionmentioning

confidence: 99%

β-Glucan Enhances the Biocontrol Efficacy of Marine Yeast Scheffersomyeces spartinae W9 against Botrytis cinerea in Strawberries

Chen

Wei

Zou

et al. 2023

JoF

Self Cite

View full text Add to dashboard Cite

The marine yeast Scheffersomyeces spartinae W9 is a promising biocontrol agent for gray mold caused by Botrytis cinerea in strawberries. Improving the biocontrol efficacy of S. spartinae W9 is necessary for its commercial application. In this study, different concentrations of β-glucan were added to the culture medium to evaluate its effect on the biocontrol efficacy of S. spartinae W9. The results showed that 0.1% β-glucan could increase the biocontrol effect of S. spartinae W9 against B. cinerea in strawberries and in vitro. We found that adding 0.1% β-glucan to the culture medium promoted the growth of S. spartinae W9 in wounds of strawberries, enhanced biofilm formation ability, and secreted more β-1,3-glucanase. In addition, 0.1% β-glucan increased the survival rate of S. spartinae W9 under oxidative, thermal, osmotic, and plasma membrane stressors. Transcriptome analysis revealed 188 differential expressed genes in S. spartinae W9 cultured with or without 0.1% β-glucan, including 120 upregulated and 68 downregulated genes. The upregulated genes were associated with stress response, cell wall formation, energy production, growth, and reproduction. Thus, culturing with 0.1% β-glucan is an effective way to improve the biocontrol ability of S. spartinae W9 against gray mold in strawberries.

show abstract

Volatile organic compounds and rapid proliferation of Candida pseudolambica W16 are modes of action against gray mold in peach fruit

Cited by 29 publications

References 42 publications

Antifungal activity of non-conventional yeasts against Botrytis cinerea and non-Botrytis grape bunch rot fungi

Antifungal activity of non-conventional yeasts against Botrytis cinerea and non-Botrytis grape bunch rot fungi

ROS Stress and Cell Membrane Disruption are the Main Antifungal Mechanisms of 2-Phenylethanol against Botrytis cinerea

β-Glucan Enhances the Biocontrol Efficacy of Marine Yeast Scheffersomyeces spartinae W9 against Botrytis cinerea in Strawberries

Contact Info

Product

Resources

About