Volatile organic compounds from Bacillus mojavensis I4 promote plant growth and inhibit phytopathogens

Ghazala, Imen; Chiab, Nour; Saidi, Mohammed Najib; Gargouri-Bouzid, Radhia

doi:10.1016/j.pmpp.2022.101887

Cited by 11 publications

(5 citation statements)

References 46 publications

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“…Volatile substances produced by Bacillus spp. reportedly inhibit phytopathogenic fungi ( Li et al, 2020 ; De la Cruz-López et al, 2022 ; Wang et al, 2022 ), affect soil microbial richness ( Yuan et al, 2017 ) and promote plant growth ( Ghazala et al, 2022 ; Rojas-Sanchez et al, 2022 ). Nevertheless, the mechanisms by which the strain and its volatile substances affect pathogenic fungi in vivo require further study.…”

Section: Discussionmentioning

confidence: 99%

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Chen

Zhang

et al. 2022

Front. Microbiol.

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Soft rot causes significant economic losses in the kiwifruit industry. This study isolated strain CTXW 7-6-2 from healthy kiwifruit tissue; this was a gram-positive bacterium that produced the red pigment pulcherrimin. The phylogenetic tree based on 16S ribosomal RNA, gyrA, rpoB, and purH gene sequences identified CTXW 7-6-2 as a strain of Bacillus subtilis. CTXW 7-6-2 inhibited hyphal growth of pathogenic fungi that cause kiwifruit soft rot, namely, Botryosphaeria dothidea, Phomopsis sp., and Alternaria alternata, by 81.76, 69.80, and 32.03%, respectively. CTXW 7-6-2 caused the hyphal surface to become swollen and deformed. Volatile compounds (VOC) produced by the strain inhibited the growth of A. alternata and Phomopsis sp. by 65.74 and 54.78%, respectively. Whole-genome sequencing revealed that CTXW 7-6-2 possessed a single circular chromosome of 4,221,676 bp that contained 4,428 protein-coding genes, with a guanine and cytosine (GC) content of 43.41%. Gene functions were annotated using the National Center for Biotechnology Information (NCBI) non-redundant protein, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes, Clusters of Orthologous Groups of proteins, Gene Ontology, Pathogen–Host Interactions, Carbohydrate-Active enZYmes, and Rapid Annotations using Subsystem Technology databases, revealing non-ribosomal pathways associated with antifungal mechanisms, biofilm formation, chemotactic motility, VOC 3-hydroxy-2-butanone, cell wall-associated enzymes, and synthesis of various secondary metabolites. antiSMASH analysis predicted that CTXW 7-6-2 can produce the active substances bacillaene, bacillibactin, subtilosin A, bacilysin, and luminmide and has four gene clusters of unknown function. Quantitative real-time PCR (qRT-PCR) analysis verified that yvmC and cypX, key genes involved in the production of pulcherrimin, were highly expressed in CTXW 7-6-2. This study elucidates the mechanism by which B. subtilis strain CTXW 7-6-2 inhibits pathogenic fungi that cause kiwifruit soft rot, suggesting the benefit of further studying its antifungal active substances.

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Section: Discussionmentioning

confidence: 99%

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Chen

Zhang

et al. 2022

Front. Microbiol.

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“…niveum ) in greenhouse pot experiments, while acetoin and 2,3-butanediol were responsible for watermelon plant growth promotion [ 96 ]. Greenhouse experiments confirmed the efficacy of B. mojavensis I4 VOCs in the suppression of Fusarium verticillioides, Fusarium graminearum, and Rhizoctonia solani in Arabidopsis thaliana plants, with significant improvements in plant growth, biomass production, and chlorophyll content [ 76 ]. The VOCs of B. cereus MH778713 (hentriacontane and 2,4-di-tert-butylphenol) reduced the disease severity of tomato Fusarium wilt ( Fusarium oxysporum ) from 88.1 ± 4.1% to only 23 ± 8.2%, followed by a several-fold increase in tomato root and shoot length as well as in the fresh and dry weight of plants in treatment with 50 μg of the bioactive VOCs [ 97 ].…”

Section: Mechanisms Of Action Of Antifungal Bacillus ...mentioning

confidence: 98%

“…Hyphal swelling, cytoplasm and protoplasm aggregation, and distortion of large amounts of balloon-shaped cells were reported for Fusarium verticillioides, Fusarium graminearum, and Rhizoctonia solani after treatment with B. mojavensis I4, with mycelial growth inhibition in the range of 16–76% [ 76 ]. Hyphae of Phyllosticta citricarpa were fattened, twisted, and deformed, with a reduced amount of conidia at the lesion site on the surface of orange plants after treatment with the VOCs produced by Bacillus sp.…”

Section: Mechanisms Of Action Of Antifungal Bacillus ...mentioning

confidence: 99%

“…Reduced sporification was observed in Aspergillus flavus in the presence of B. cereus SV40, B. subtilis SV36/2, and B. coagulans SV95 VOCs [ 1 ]. A significant decrease in spore density of Fusarium verticillioides and Fusarium graminearum was observed after treatment with B. mojavensis I4 VOCs [ 76 ]. The different concentrations of 1%, 3%, 5%, 7%, and 10% of the VOCs produced by B. velezensis CE 100 reduced the spore germination of Colletotrichum gloeosporioides (walnut and jujube antrachnose) by 15.4%, 18.9%, 25.4%, 30.0%, and 36.4%, respectively, with remarkably reduced germ tube elongation [ 75 ].…”

Section: Mechanisms Of Action Of Antifungal Bacillus ...mentioning

confidence: 99%

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Bacillus VOCs in the Context of Biological Control

2023

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A contemporary agricultural production system relying on heavy usage of agrochemicals represents a questionable outlook for sustainable food supply in the future. The visible negative environmental impacts and unforeseen consequences to human and animal health have been requiring a shift towards the novel eco-friendly alternatives for chemical pesticides for a while now. Microbial-based biocontrol agents have shown a promising potential for plant disease management. The bacteria of the genus Bacillus have been among the most exploited microbial active components due to several highly efficient mechanisms of action against plant pathogens, as well as a palette of additional plant-beneficial mechanisms, together with their suitable properties for microbial biopesticide formulations. Among other bioactive metabolites, volatile organic compounds (VOCs) have been investigated for their biocontrol applications, exhibiting the main advantage of long-distance effect without the necessity for direct contact with plants or pathogens. The aim of this study is to give an overview of the state-of-the-art in the field of Bacillus-based VOCs, especially in terms of their antibacterial, antifungal, and nematicidal action as the main segments determining their potential for biocontrol applications in sustainable agriculture.

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“…Most of the chemicals identified from three different Bacillus species were derived from volatile compounds, such as esters, alkaloids, ethers, and phenolics, and shared structural similarities with natural products of bacterial and plant origin. Many volatile organic compounds are major constituents of the bacterial strain and have been shown to have properties against phytopathogens [ 37 , 38 ]. Our results prove that Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Some Bacillus Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency

et al. 2023

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Numerous natural habitats, such as soil, air, fermented foods, and human stomachs, are home to different Bacillus strains. Some Bacillus strains have a distinctive predominance and are widely recognized among other microbial communities, as a result of their varied habitation and physiologically active metabolites. The present study collected vegetable products (potato, carrot, and tomato) from local markets in Almaty, Kazakhstan. The bacterial isolates were identified using biochemical and phylogenetic analyses after culturing. Our phylogenetic analysis revealed three Gram-positive bacterial isolates BSS11, BSS17, and BSS19 showing 99% nucleotide sequence similarities with Bacillus subtilis O-3, Bacillus subtilis Md1-42, and Bacillus subtilis Khozestan2. The crude extract was prepared from bacterial isolates to assess the antibiotic resistance potency and the antimicrobial potential against various targeted multidrug-resistant strains, including Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus group B, Streptococcus mutans, Candida albicans, Candida krusei, Pseudomonas aeruginosa, Shigella sonnei, Klebsiella pneumoniae, Salmonella enteritidis, Klebsiella aerogenes, Enterococcus hirae, Escherichia coli, Serratia marcescens, and Proteus vulgaris. This study found that the species that were identified have the ability to produce antibiotic chemicals. Additionally, the GC–MS analysis of three bacterial extracts revealed the presence of many antibiotic substances including phenol, benzoic acid, 1,2-benzenedicarboxylic acid and bis(2-methylpropyl), methoxyphenyl-oxime, and benzaldehyde. This work sheds light on the potential of Bacillus to be employed as an antimicrobial agent to target different multidrug-resistant bacterial strains. The results indicate that market vegetables may be a useful source of strains displaying a range of advantageous characteristics that can be used in the creation of biological antibiotics.

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Volatile organic compounds from Bacillus mojavensis I4 promote plant growth and inhibit phytopathogens

Cited by 11 publications

References 46 publications

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Biocontrol potential of Bacillus subtilis CTXW 7-6-2 against kiwifruit soft rot pathogens revealed by whole-genome sequencing and biochemical characterisation

Bacillus VOCs in the Context of Biological Control

Molecular Characterization of Some Bacillus Species from Vegetables and Evaluation of Their Antimicrobial and Antibiotic Potency

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