Volatile organic compounds produced by Aureobasidium pullulans induce electrolyte loss and oxidative stress in Botrytis cinerea and Alternaria alternata

Don, Sashika M. Yalage; Schmidtke, Leigh; Gambetta, Joanna M.; Steel, Christopher

doi:10.1016/j.resmic.2020.10.003

Cited by 34 publications

(20 citation statements)

References 32 publications

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“…Rezende reported that fungi exposed to 2methyl-1-butanol and 3-methyl-1-butanol experienced oxidative stress and changes in plasma membrane permeability [22]. Yalage Don et al demonstrated that exposure to a mixture of VOCs, including ethanol, 2methyl-1-propanol, 3-methyl-1-butanol, and 2-phenylethanol, resulted in ROS accumulation and electrolyte leakage in B. cinerea and Alternaria alternata [23]. Among the selected strains in this study, Aureobasidium sp.…”

Section: Discussionmentioning

confidence: 99%

Screening of Volatile Organic Compound-Producing Yeasts and Yeast-Like Fungi against Aflatoxigenic Aspergillus flavus

Nasanit

Jaibangyang

Onwibunsiri

et al. 2022

Microbiol. Biotechnol. Lett.

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Aureobasidium sp. DMKU-PAL144, Rhodotorula sp. DMKU-PAL99, and Solicococcus keelungensis DMKU-PAL84. VOCs produced by these microorganisms ranged from 4 to 14 compounds and included alcohols, alkenes, aromatics, esters and furans. The major VOCs produced by the closely related Aureobasidium strains were found to bedistinct. Moreover, 2-phenylethanol was the most abundant compound generated by Aureobasidium sp. DMKU-PAL120, while methyl benzeneacetate was the major compound emitted from Aureobasidium sp. DMKU-PAL144. On the other hand, 2-methyl-1-butanol and 3-methyl-1-butanol were significant compounds produced by the other three genera. These antagonists apparently inhibited A. flavus sporulation and mycelial development. Additionally, the reduction of the AFB 1 in the fungal-contaminated rice grains was observed after co-incubation with these VOC-producing strains and ranged from 37.7 ± 8.3% to 60.3 ± 3.4%. Our findings suggest that these same microorganisms are promising biological control agents for use against aflatoxin-producing fungi in rice and other agricultural products.

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Section: Discussionmentioning

confidence: 99%

Screening of Volatile Organic Compound-Producing Yeasts and Yeast-Like Fungi against Aflatoxigenic Aspergillus flavus

Nasanit

Jaibangyang

Onwibunsiri

et al. 2022

Microbiol. Biotechnol. Lett.

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“…Citric acid yield can also be improved through the manipulation of membrane permeability by supplementing a variety of quaternary ammonium compounds or amine oxides to the fermentation medium in amounts from about 5 to about 50 parts per million (= mg L −1 ) when Fe 2+ concentration is over 0.2 mg L −1 [94]. The biochemical basis for the effect of membrane affecting agents for counteracting the effect of manganese ions on citric acid production is not known yet; however, recent studies demonstrated that they evoke oxidative stress [95,96] and therefore mimick one of the major effects of manganese deficiency.…”

Section: Metal Ions-related Aspects Of Submerged Organic Acid Fermentationsmentioning

confidence: 99%

The Role of Metal Ions in Fungal Organic Acid Accumulation

2021

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Organic acid accumulation is probably the best-known example of primary metabolic overflow. Both bacteria and fungi are capable of producing various organic acids in large amounts under certain conditions, but in terms of productivity-and consequently, of commercial importance-fungal platforms are unparalleled. For high product yield, chemical composition of the growth medium is crucial in providing the necessary conditions, of which the concentrations of four of the first-row transition metal elements, manganese (Mn2+), iron (Fe2+), copper (Cu2+) and zinc (Zn2+) stand out. In this paper we critically review the biological roles of these ions, the possible biochemical and physiological consequences of their influence on the accumulation of the most important mono-, di- and tricarboxylic as well as sugar acids by fungi, and the metal ion-related aspects of submerged organic acid fermentations, including the necessary instrumental analytics. Since producing conditions are associated with a cell physiology that differs strongly to what is observed under “standard” growth conditions, here we consider papers and patents only in which organic acid accumulation levels achieved at least 60% of the theoretical maximum yield, and the actual trace metal ion concentrations were verified.

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“…The species A. pullulans is also known as an effective biocontrol agent that is thought to employ a range of mechanisms to suppress the growth of other microorganisms [9]. These mechanisms include direct competition [15][16][17], the secretion of enzymes and secondary metabolites [4-8, 18, 19], the production of volatile compounds [20][21][22][23][24][25], as well as the induction of resistance in crop plants [26,27]. Many Aureobasidium isolates show strong biocontrol activity against destructive plant diseases such as moulds, rots and blights [28][29][30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

et al. 2021

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Aureobasidium pullulans is an extremotolerant, cosmopolitan yeast-like fungus that successfully colonises vastly different ecological niches. The species is widely used in biotechnology and successfully applied as a commercial biocontrol agent against postharvest diseases and fireblight. However, the exact mechanisms that are responsible for its antagonistic activity against diverse plant pathogens are not known at the molecular level. Thus, it is difficult to optimise and improve the biocontrol applications of this species. As a foundation for elucidating biocontrol mechanisms, we have de novo assembled a high-quality reference genome of a strongly antagonistic A. pullulans strain, performed dual RNA-seq experiments, and analysed proteins secreted during the interaction with the plant pathogen Fusarium oxysporum. Based on the genome annotation, potential biocontrol genes were predicted to encode secreted hydrolases or to be part of secondary metabolite clusters (e.g., NRPS-like, NRPS, T1PKS, terpene, and β-lactone clusters). Transcriptome and secretome analyses defined a subset of 79 A. pullulans genes (among the 10,925 annotated genes) that were transcriptionally upregulated or exclusively detected at the protein level during the competition with F. oxysporum. These potential biocontrol genes comprised predicted secreted hydrolases such as glycosylases, esterases, and proteases, as well as genes encoding enzymes, which are predicted to be involved in the synthesis of secondary metabolites. This study highlights the value of a sequential approach starting with genome mining and consecutive transcriptome and secretome analyses in order to identify a limited number of potential target genes for detailed, functional analyses.

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Volatile organic compounds produced by Aureobasidium pullulans induce electrolyte loss and oxidative stress in Botrytis cinerea and Alternaria alternata

Cited by 34 publications

References 32 publications

Screening of Volatile Organic Compound-Producing Yeasts and Yeast-Like Fungi against Aflatoxigenic Aspergillus flavus

Screening of Volatile Organic Compound-Producing Yeasts and Yeast-Like Fungi against Aflatoxigenic Aspergillus flavus

The Role of Metal Ions in Fungal Organic Acid Accumulation

Genome, transcriptome and secretome analyses of the antagonistic, yeast-like fungus Aureobasidium pullulans to identify potential biocontrol genes

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