Voltage-dependent ionic currents in solitary horizontal cells isolated from cat retina

Ueda, Yoshiki; Kaneko, Akimichi; Kaneda, Makoto

doi:10.1152/jn.1992.68.4.1143

Cited by 58 publications

(69 citation statements)

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“…Dissociated bipolar cells were prepared according to the procedures described previously (Ueda et al, 1992), with some modifications. Eyes from pups of postnatal days (PD) 12-20 were enucleated, and the sclera and choroid were peeled off.…”

Section: Methodsmentioning

confidence: 99%

The mGluR6 5′ Upstream Transgene Sequence Directs a Cell-Specific and Developmentally Regulated Expression in Retinal Rod and ON-Type Cone Bipolar Cells

et al. 1997

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We generated transgenic mice, using 9.5 kilobase pairs of the 5Ј upstream sequence from the mouse metabotropic glutamate receptor subtype 6 (mGluR6) gene fused to the ␤-galactosidase (lacZ ) reporter gene, and investigated the promoter function of the cell-specific and developmentally regulated expression of mGluR6. Most of the independent transgenic lines commonly showed the lacZ expression in the defined cell layers of the retina, and four transgenic lines were characterized in detail for cell-specific lacZ expression patterns by X-gal staining and lacZ immunostaining. The lacZ-expressing retinal cells were classified into two cell types. One cell type was identified as rod bipolar cells on the basis of colocalization of protein kinase C (PKC) immunoreactivity and morphological criteria. The other cell type was PKC-immunonegative and resided at the cell layers corresponding precisely to ON-type cone bipolar cells. The latter bipolar cells were found to exist as a large cell population comparable to rod bipolar cells. This observation was confirmed by coimmunostaining of dissociated retinal cells with the lacZ and PKC antibodies. The ontogeny analysis indicated that the lacZ expression completely agrees with a temporal expression pattern of mGluR6 during retinal development. This study demonstrates that the mGluR6 5Ј upstream genomic sequence is capable of directing a cell-specific and developmentally regulated expression of mGluR6 in ON-type bipolar cells and supports the view that mGluR6 is responsible for ON responses in both the rod and cone systems.

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Section: Methodsmentioning

confidence: 99%

The mGluR6 5′ Upstream Transgene Sequence Directs a Cell-Specific and Developmentally Regulated Expression in Retinal Rod and ON-Type Cone Bipolar Cells

et al. 1997

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“…Voltage-gated sodium channels have been identified on isolated horizontal cells (Shingai and Christensen 1983;Ueda et al 1992) and on human rod photoreceptors (Kawai et al 2001), but there is no evidence that sodium currents shape light responses of these neurons. If salamander photoreceptors or horizontal cells express voltage-gated sodium channels, then TTX could alter transmission from photoreceptors to secondorder neurons.…”

Section: Ttx Does Not Reduce Glutamate Release From Photoreceptor Termentioning

confidence: 99%

“…There are reports of voltage-gated sodium channels in certain classes of photoreceptors, horizontal cells, and cone bipolar cells in other species (Kawai et al 2001;Pan and Hu 2000;Shingai and Christensen 1983;Ueda et al 1992;Zenisek et al 2001). In the salamander, these channels have been observed on ganglion and amacrine cells but never on other retinal neurons (Barnes and Werblin 1986;Lukasiewicz and Werblin 1988).…”

Section: Voltage-gated Sodium Channels In Other Retinal Neuronsmentioning

confidence: 99%

“…Because functional voltage-gated sodium channels have been found on photoreceptors (Kawai et al 2001) and isolated horizontal cells (Shingai and Christensen 1983;Ueda et al 1992), we considered the possibility that TTX could alter photoreceptor output. Our recordings from bipolar cells showed that TTX did not affect their light-evoked EPSCs, indicating that photoreceptor to bipolar cell transmission did not depend on regenerative sodium currents.…”

Section: Voltage-gated Sodium Channels In Other Retinal Neuronsmentioning

confidence: 99%

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Spike-Dependent GABA Inputs to Bipolar Cell Axon Terminals Contribute to Lateral Inhibition of Retinal Ganglion Cells

Shields

Lukasiewicz

2003

Journal of Neurophysiology

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The inhibitory surround signal in retinal ganglion cells is usually attributed to lateral horizontal cell signaling in the outer plexiform layer (OPL). However, recent evidence suggests that lateral inhibition at the inner plexiform layer (IPL) also contributes to the ganglion cell receptive field surround. Although amacrine cell input to ganglion cells mediates a component of this lateral inhibition, it is not known if presynaptic inhibition to bipolar cell terminals also contributes to surround signaling. We investigated the role of presynaptic inhibition by recording from bipolar cells in the salamander retinal slice. TTX reduced light-evoked GABAergic inhibitory postsynaptic currents (IPSCs) in bipolar cells, indicating that presynaptic pathways mediate lateral inhibition in the IPL. Photoreceptor and bipolar cell synaptic transmission were unaffected by TTX, indicating that its main effect was in the IPL. To rule out indirect actions of TTX, we bypassed lateral signaling in the outer retina by either electrically stimulating bipolar cells or by puffing kainate (KA) directly onto amacrine cell processes lateral to the recorded cell. In bipolar and ganglion cells, TTX suppressed laterally evoked IPSCs, demonstrating that both pre- and postsynaptic lateral signaling in the IPL depended on action potentials. By contrast, locally evoked IPSCs in both cell types were only weakly suppressed by TTX, indicating that local inhibition was not as dependent on action potentials. Our results show a TTX-sensitive lateral inhibitory input to bipolar cell terminals, which acts in concert with direct lateral inhibition to give rise to the GABAergic surround in ganglion cells.

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“…Single bipolar cells were isolated from retinas of pink-eyed RCS rats between postnatal day 25 and 55 and of normal pigmented rats according to the procedure used by Ueda et al [1992] with minor modifications. 500 µl of the cell suspension were transferred into a plastic culture dish previously filled with 2 ml of the standard extracellular solution which contained (in mM): 10 KCl, 130 NaCl, 1 MgCl 2 , 2 CaCl 2 , 5 N-2-hydroxyethylpiperazine-N′-2-ethane-sulfonic acid (HEPES), and 10 glucose.…”

Section: Isolated Bipolar Cellsmentioning

confidence: 99%

Impaired Function of Bipolar Cells in the Royal College of Surgeons Rat

Hanitzsch

Zeumer²,

Lichtenberger

et al. 1998

Cells Tissues Organs

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The retina of the Royal College of Surgeons (RCS) strain of rat, which is being used as an animal model for human retinal degenerations, has been employed in the study of the function of second order neurons. By about the 33rd postnatal day the dendritic branching of isolated bipolar cells is more sparse than in bipolar cells of the normal rat retina, but their GABA channels are as in the normal rat retina. The normally occurring light-induced distal potassium increase has been used as the indicator of the functional competence of second order neurons in the isolated RCS rat retina. These are dependent upon the integrity of ionotropic and metabotropic synapses. At about the 22nd postnatal day MgCl₂ enlarges the light-induced distal potassium increase in the young RCS rat retina as in the normal rat retina. It seems that MgCl₂ does not block the metabotropic synapses of on-bipolar cells. At about postnatal day 33, at which time the photoreceptors of the RCS rat retina had become severely damaged, the size of this light-induced distal potassium increase was not changed, but it was abolished by MgCl₂. This indicates that bipolar cells are still active but that the synaptic function of on-bipolar cells has become vulnerable to MgCl₂. The conclusion is that at a time when photoreceptor degeneration is already severe bipolar cells are still active, but that on-bipolars, mainly rod bipolar cells, have some functional deficit.

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Voltage-dependent ionic currents in solitary horizontal cells isolated from cat retina

Cited by 58 publications

References 0 publications

The mGluR6 5′ Upstream Transgene Sequence Directs a Cell-Specific and Developmentally Regulated Expression in Retinal Rod and ON-Type Cone Bipolar Cells

The mGluR6 5′ Upstream Transgene Sequence Directs a Cell-Specific and Developmentally Regulated Expression in Retinal Rod and ON-Type Cone Bipolar Cells

Spike-Dependent GABA Inputs to Bipolar Cell Axon Terminals Contribute to Lateral Inhibition of Retinal Ganglion Cells

Impaired Function of Bipolar Cells in the Royal College of Surgeons Rat

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