Voltammetric Behavior and Reliable Method for the Determination of Coccidiostat Robenidine in Animal Feed and Poultry Meat

Abstract

The voltammetric behaviour of coccidiostat robenidine was investigated. The outcomes have revealed that reduction of robenidine causes an appearance of one diffusion‐controlled cathodic peak on a static mercury drop electrode (SMDE) and a silver solid amalgam electrode (p‐AgSAE). The influence of pH, a supporting electrolyte, a scan rate and accumulation parameters was evaluated. The mechanism of robenidine reduction involving two electrons and one proton was studied for the first time. The calibration curves with the wide linear concentration ranges (more than two concentration orders) were obtained under optimized experimental conditions and operating parameters. The reduction current linearly increases when increasing the concentration of coccidiostat. The methods of voltammetric determination of robenidine in feed and meat have been developed. The limits of quantitation of the methods of robenidine determination in feed are 9.5 mg/kg and 2.5 mg/kg on SMDE and p‐AgSAE, respectively. Calculated recoveries ranges of robenidine were between 88.8 % and 101.5 %.