Volume-sensitive taurine transport in fish erythrocytes

Fincham, Daron A.; Wolowyk, Michael W.; Young, James D.

doi:10.1007/bf01869333

Cited by 114 publications

(51 citation statements)

References 49 publications

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“…This contribution has been described in invertebrates and also in mammalian tissues such as cardiac cells (Thurston, Hauhart & Naccarato, 1981), Ehrlich cells (Hoffmann & Lambert, 1983;Lambert, 1984;Lambert & Hoffmannn, 1993) (Fugelli & Thoroed, 1986;Kirk, Ellory & Young, 1992), the skate (Goldstein & Brill, 1990, the eel (Fincham, Wolowyk & Young, 1987) and the trout (Garcia-Romeu, Cossins & Motais, 1991), and also in the cyclostomes, hagfish and lamprey (Brill, Musch & Goldstein, 1992).…”

Section: Introductionmentioning

confidence: 84%

“…It is usually observed that RVD is associated with stimulation of the Na+-independent efflux of taurine, while the Na+-dependent influx is actually diminished (Hoffmann & Lambert, 1983;Fugelli & Thoroed, 1986;Fincham et al 1987). Fish erythrocytes differ from other cells in that hyposmolarity activates not only the output but also the uptake of taurine via a sodium-independent mechanism (Fugelli & Thoroed, 1986;Fincham et al 1987). Trout hepatocytes may be unique in that the taurine efflux accelerated by cell swelling relates to the presence of both sodium and chloride in the medium, as shown in Table 2.…”

Section: Discussionmentioning

confidence: 99%

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Effects of hyposmotic shock on taurine transport in isolated trout hepatocytes

Michel¹,

Fossat²,

Porthé-Nibelle³

et al. 1994

Experimental Physiology

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SUMMARYIsolated trout hepatocytes exposed to hypotonic medium undergo a regulatory volume decrease (RVD) that occurs via two separate routes, K+-Cl-cotransport and amino acid release, the ion efflux accounting for 70 % of the total osmolyte loss. Taurine, glutamine and glutamic acid are the most important and represent 73 % of the total amino acid content (53 mmol (1 cell water)-').The osmolarity-sensitive release of amino acids was studied using [3H]taurine. Kinetic studies indicated two components for taurine influx: a linear Na+-independent transport and a saturable Na+-dependent system with a Michaelis-Menten constant (K.) of 122 fSM and a maximum velocity (Vmax) of 31-2 pmol (mg protein)-1 min-'. This second way of uptake was also chloride dependent and indicated an apparent coupling ratio Na+: Cl-: taurine of 2:1:1. The latter component and the taurine efflux were stimulated during RVD, leading to intracellular amino acid loss. Taurine efflux activation during volume recovery was transient and also dependent on the presence of both Na+ and Cl-in the extracellular medium. Furthernore, taurine release and RVD were slowed down when Ca2l was omitted from the medium. These results suggested two distinct and complementary mechanisms for volume regulation in trout hepatocytes during hypotonic conditions.

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Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Effects of hyposmotic shock on taurine transport in isolated trout hepatocytes

Michel¹,

Fossat²,

Porthé-Nibelle³

et al. 1994

Experimental Physiology

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“…For example, a net loss of taurine, a non-protein amino acid, occurs during a RVD in fish red blood cells (e.g. see Fincham, Wolowyk & Young, 1987;Goldstein & Brill, 1990). Indeed, taurine and other amino acids, notably glycine and alanine, have been shown to contribute to a RVD in some mammalian cells, such as Ehrlich ascites tumour cells and rat heart (Thurston, Hauhart & Naccarato, 1981;Hoffmann & Lambert, 1983).…”

Section: Introductionmentioning

confidence: 99%

The effect of a hyposmotic shock on amino acid efflux from lactating rat mammary tissue: stimulation of taurine and glycine efflux via a pathway distinct from anion exchange and volume‐activated anion channels

Shennan¹,

McNeillie²,

Curran³

1994

Experimental Physiology

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SUMMARYWe have examined the effect of a hyposmotic shock, and thus cell swelling, upon the efflux of amino acids, SO42-and 1-from lactating mammary tissue. A hyposmotic challenge increased the efflux of taurine and glycine via a 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS)-sensitive pathway. It appears that these amino acids do not exit via an anion-exchange mechanism following cell swelling because sulphate efflux, which uses a DIDS-sensitive exchange mechanism, was unaffected. The hyposmotic-induced efflux of taurine was not dependent upon the Na+ gradient and was not influenced by the nature of the anion in the incubation medium. In addition, taurine efflux was stimulated by incubating mammary tissue in an isosmotic medium that contained urea, suggesting that cell swelling is the stimulating factor rather than a decrease in osmolality per se. The results suggest that mammary tissue uses taurine and glycine as a means of regulating cell volume following swelling. In contrast, the efflux of glutamic acid, alanine and ac-aminoisobutyric acid was unaffected by a hyposmotic challenge.Similarly, the efflux of 1-was unaffected by such a challenge. The results suggest that volumeactivated amino acid transport in lactating rat mammary tissue is distinct from volume-regulated anion channels.

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“…Fish erythrocytes respond to hypotonic swelling by inducing a loss of K ϩ and Cl Ϫ and also a transport of taurine and polyols (2)(3)(4)(5)(6)(7)(8) similarly affected by various anion transport blockers (5,(7)(8)(9). Recent observations (10) show that: (i) expression of the trout anion exchanger AE1 (tAE1) in Xenopus oocyte elicits, in addition to anion exchange activity, both anion conductance and appearance of a passive taurine transport; (ii) a very tight correlation exists between expression of anion conductance and taurine transport; (iii) compounds that inhibit anion conductance also inhibit taurine transport; (iv) expression of the anion exchanger (mAE1) of mouse erythrocyte, a cell that does not release organic osmolytes in response to swelling, elicits anion exchange activity but neither conductance nor taurine transport.…”

mentioning

confidence: 99%

Transport of uncharged organic solutes in Xenopus oocytes expressing red cell anion exchangers (AE1s)

Fiévet

Perset

Gabillat

et al. 1998

Proc. Natl. Acad. Sci. U.S.A.

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When expressed in Xenopus oocytes, the trout red cell anion exchanger tAE1, but not the mouse exchanger mAE1, elicited a transport of electroneutral solutes (sorbitol, urea) in addition to the expected anion exchange activity. Chimeras constructed from mAE1 and tAE1 allowed us to identify the tAE1 domains involved in the induction of these transports. Expression of tAE1 (but not mAE1) is known to generate an anion conductance associated with a taurine transport. The present data provide evidence that (i) the capacity of tAE1 and tAE1 chimeras to generate urea and sorbitol permeability also was associated with an anion conductance; (ii) the same inhibitors affected both the permeability of solutes and anion conductance; and (iii) no measurable water transport was associated with the tAE1-dependent conductance. These results support the view that fish red blood cells, to achieve cell volume regulation in response to hypotonic swelling, activate a tAE1-associated anion channel that can mediate the passive transport of taurine and electroneutral solutes.Hypotonically swollen cells recover their volume (regulatory volume decrease) by releasing intracellular solutes (ions and organic compounds) and obligated water. Taurine, an amino acid that is present in various cells at concentrations of up to 40 mM, is often the primary organic osmolyte involved in regulatory volume decrease. But, in some cells, polyols, such as sorbitol and myoinositol, play an important role (1). Volumeactivated amino acid and polyol efflux mechanisms share two characteristics (1): (i) they are mediated by passive, Naindependent transport pathways having the characteristics of anion channels and (ii) they are similarly affected by various anion transport blockers, suggesting that transport of these structurally unrelated compounds may be mediated by a single pathway.Fish erythrocytes respond to hypotonic swelling by inducing a loss of K ϩ and Cl Ϫ and also a transport of taurine and polyols (2-8) similarly affected by various anion transport blockers (5, 7-9). Recent observations (10) show that: (i) expression of the trout anion exchanger AE1 (tAE1) in Xenopus oocyte elicits, in addition to anion exchange activity, both anion conductance and appearance of a passive taurine transport; (ii) a very tight correlation exists between expression of anion conductance and taurine transport; (iii) compounds that inhibit anion conductance also inhibit taurine transport; (iv) expression of the anion exchanger (mAE1) of mouse erythrocyte, a cell that does not release organic osmolytes in response to swelling, elicits anion exchange activity but neither conductance nor taurine transport. Taken together, these results indicate that the tAE1-induced channel mediates a taurine transport and suggest that, in fish red blood cells, the volume-activated taurine transport is associated with the anion exchanger (band 3 protein).Hyposmotic swelling of trout red blood cells induces not only taurine transport (4) but also a transport of sorbitol that is inhibited...

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Volume-sensitive taurine transport in fish erythrocytes

Cited by 114 publications

References 49 publications

Effects of hyposmotic shock on taurine transport in isolated trout hepatocytes

Effects of hyposmotic shock on taurine transport in isolated trout hepatocytes

The effect of a hyposmotic shock on amino acid efflux from lactating rat mammary tissue: stimulation of taurine and glycine efflux via a pathway distinct from anion exchange and volume‐activated anion channels

Transport of uncharged organic solutes in Xenopus oocytes expressing red cell anion exchangers (AE1s)

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