Volumetric absorptive microsampling (VAMS) as an alternative to conventional dried blood spots in the quantification of miltefosine in dried blood samples

Kip, Anke E; Kiers, K.; Rosing, Hilde; Schellens, Jan H.M.; Beijnen, Jos H.; Dorlo, Thomas P. C.

doi:10.1016/j.jpba.2016.12.012

Cited by 73 publications

(69 citation statements)

References 17 publications

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“…The water loss slows down or stops most degradation and metabolic processes, thus usually increasing analyte stability and allowing storage at room temperature (RT) for long periods of time [15,16]. The small dried sample can be accommodated in very small storage spaces and be shipped safely and feasibly; it also reduces solvent consumption and is thus cheaper and more sustainable [17][18][19][20][21]. VAMS use in particular is designed to be easily automated, and to sample accurate blood volumes without bias due to variable hematocrit values, which is a major drawback of other microsampling techniques, such as dried blood spots (DBS) on cards [22,23].…”

Section: Introductionmentioning

confidence: 99%

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

2020

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Reliable, feasible analytical methods are needed for forensic and anti-doping testing of cocaine and its most important metabolites, benzoylecgonine, ecgonine methyl ester, and cocaethylene (the active metabolite formed in the presence of ethanol). An innovative workflow is presented here, using minute amounts of dried blood or plasma obtained by volumetric absorptive microsampling (VAMS), followed by miniaturized pretreatment by dispersive pipette extraction (DPX) and LC-MS/MS analysis. After sampling 20 µL of blood or plasma with a VAMS device, the sample was dried, extracted, and loaded onto a DPX tip. The DPX pretreatment lasted less than one minute and after elution with methanol the sample was directly injected into the LC-MS/MS system. The chromatographic analysis was carried out on a C8 column, using a mobile phase containing aqueous formic acid and acetonitrile. Good extraction yield (> 85%), precision (relative standard deviation, RSD < 6.0%) and matrix effect (< 12%) values were obtained. Analyte stability was outstanding (recovery > 85% after 2 months at room temperature). The method was successfully applied to real blood and plasma VAMS, with results in very good agreement with those of fluid samples. The method seems suitable for the monitoring of concomitant cocaine and ethanol use by means of plasma or blood VAMS testing.

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Section: Introductionmentioning

confidence: 99%

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

2020

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“…Daughter ions were selected by studying MS/MS spectra obtained by summing‐up collision‐induced dissociation spectra at various collision energies, in the range from 5 to 100 eV. In addition to these experimental observations, data available in the literature were also used as a guide to transition selection [12‐24].…”

Section: Resultsmentioning

confidence: 99%

“…Classically, liquid whole blood, serum or plasma samples (LPs) (obtained from brachial venipuncture) are employed in the TDM of antipsychotics and concentration reference ranges are available for the interpretation of TDM results [2]. Recently, dried blood (DB), serum, or dried plasma (DP) spot samples have been shown as convenient alternatives to conventional liquid samples for drug measurement [15‐31]. They are easier to collect (even by a self‐directed simple finger pricking), easier to conserve, and to ship abroad because they do not require refrigeration, since analyte degradation is minimized in the absence of water [16].…”

Section: Introductionmentioning

confidence: 99%

Multiplexed therapeutic drug monitoring of antipsychotics in dried plasma spots by LC‐MS/MS

Ruggiero¹,

Ramirez²,

Ramazzotti³

et al. 2020

J of Separation Science

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In this work, a convenient method for the therapeutic monitoring of seven common antipsychotic drugs in "dried plasma spot" samples has been developed. It is based on the liquid chromatography tandem mass spectrometry technique, operating in multiple reaction monitoring mode, and a straightforward procedure for the simultaneous extraction of all antipsychotics in a single step, with high extraction yield. The method was fully validated with proper accuracy, precision, selectivity and sensitivity, for all the drugs. Limits of quantification were 0.12, 1.09, 1.46, 1.47, 5.70, 1.32, 1.33 µg/L for haloperidol, aripiprazole, olanzapine, quetiapine, clozapine, risperidone, and paliperidone, respectively. Accuracy, intra-and interday precision values were <10% for all drugs at all concentration levels examined. The method was tested in the analysis of 30 plasma samples from real patients for each drug. The proposed analytical approach, by combining practical and logistical advantages of microsampling with liquid chromatography tandem mass spectrometry analytical performance, could offer an ideal strategy for accurate and timely therapeutic drug monitoring of antipsychotic drugs in most clinical settings, even in remote centers and/or in out-patient settings, bringing so many potential improvements in psychiatric patient care. K E Y W O R D Santipsychotic drugs, dried plasma spots, liquid chromatography-mass spectrometry, therapeutic drug monitoring 1440

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“…Finally, an effective scaling-up can be achieved when relevant POC devices are available. All these benefits can potentially improve treatment outcomes, especially for patients who are in remote areas or require regular diagnostic services [106][107][108] . The platform consists of a handheld device and a disposable cartridge.…”

Section: Benefits Of a Decentralized Model And How Microsampling Is Dmentioning

confidence: 99%

“…In the epidemiological study, an early version of the absorbent microbiopsy was applied on the lesion sites of Leishmaniasis patients in Ghana and Ethiopia. Leishmaniasis is a parasitic disease and is commonly found in rural areas in North Africa 54,106 . Skin lesion, mainly ulcer, is one of the symptoms of Leishmaniasis.…”

Section: Research Aimsmentioning

confidence: 99%

Advances of the skin microbiopsy for blood and skin sampling with streamlined handling and fabrication settings

Lei¹

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List of Figures List of Tables 1. Literature review, hypotheses and aims 1.1. Literature review 1.2. Hypotheses 1.3. Aims 2. Development of the absorbent microbiopsy for minimally invasive blood and skin sampling 2.1. Introduction 2.2. Materials and methods 2.3. Results 2.4. Conclusion 3. Fabrication scale-up with 3D printing technologies 3.1. Introduction 3.2. Materials and methods 3.3. Results 3.4. Conclusion 4. Plunger redesigning for improving inhouse fabrication capability 4.1. Introduction 4.2. Materials and methods 4.3. Results 4.4. Conclusion 5. Exploring approaches for improving the sample processing experience 5.1. Introduction 5.2. Materials and methods 5.3. Results 5.4. Conclusion 6. Conclusions, general discussion and future plans 6.1. Comparisons of current sampling approaches 6.2. Development of the absorbent microbiopsy for minimally invasive blood and skin sampling 6.3. Fabrication scale-up with 3D printing technologies 6.4. Plunger redesigning for improving inhouse fabrication capability 6.5. Exploring approaches for improving the user experience 6.6. General discussion and future directions 7. References 8. Appendices Appendix 1-Comparison of RNA recoveries between Whatman paper and the entire absorbent microbiopsy Appendix 2-Absorption capacity testing of Whatman paper Appendix 3-ELISA detection limit experiment Appendix 4-Modified skin microbiopsy permitted easier microscopic observation of sample after application Appendix 5-Plunger with removable handlebar improved handling experience Appendix 6-Combining skin and absorbent microbiopsies into one device permitted blood sampling, but the skin channel function required further optimization Appendix 7-Schematic illustration of the absorbent microbiopsy design for the pen applicator (chapter 2) Appendix 8-Schematic illustration of the absorbent microbiopsy array (chapter 3) Appendix 9-AutoCAD drawings of the pen applicator parts (chapter 3) Appendix 10-AutoCAD drawings of folding jig parts design 5 (chapter 3) Appendix 11-AutoCAD drawings of 3D-printed plunger design 2 (chapter 4) Appendix 12-Schematic illustration of the absorbent microbiopsy for acrylic sheet plunger design 8 (chapter 4) Appendix 13-Schematic illustration of acrylic sheet plunger design 8 Appendix 14-Accepted manuscript: Publication on Biomedical Microdevices Appendix 15-Accepted manuscript: Publication on Journal of Visual Experiment UV Ultraviolet VAMS Volumetric absorptive microsampling 2D Two-dimensional 3D Three-dimensional %CV Coefficients of variation Chapter 1: Literature review and hypotheses 1.1. Literature review The following literature review is modified from a published manuscript. A copy of the published version can be found in chapter 8.

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Volumetric absorptive microsampling (VAMS) as an alternative to conventional dried blood spots in the quantification of miltefosine in dried blood samples

Cited by 73 publications

References 17 publications

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

Blood and Plasma Volumetric Absorptive Microsampling (VAMS) Coupled to LC-MS/MS for the Forensic Assessment of Cocaine Consumption

Multiplexed therapeutic drug monitoring of antipsychotics in dried plasma spots by LC‐MS/MS

Advances of the skin microbiopsy for blood and skin sampling with streamlined handling and fabrication settings

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