Vortex-assisted liquid-liquid–liquid microextraction followed by high performance liquid chromatography for the simultaneous determination of fourteen phenolic acids in honey, iced tea and canned coffee drinks

Shalash, Marwan; Makahleh, Ahmad; Salhimi, Salizawati Muhamad; Saad, Bahruddin

doi:10.1016/j.talanta.2017.06.039

Cited by 44 publications

(17 citation statements)

References 42 publications

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“…The extraction (vortex) time is a critical factor in the VA‐SI‐LLME procedure as it influences the mass transfer of target analytes and thus affects the extraction recoveries . A vortex mixer was utilized for the extraction process as its vigorous agitation helps to increase the extraction recoveries of OPP metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…The extraction (vortex) time is a critical factor in the VA-SI-LLME procedure as it influences the mass transfer of target analytes and thus affects the extraction recoveries. 31,32 A vortex mixer was utilized for the extraction process as its vigorous agitation helps to increase the extraction recoveries of OPP metabolites. The influence of the vortex agitation time on the extraction recoveries of OPP metabolites from urine samples was investigated by varying the time at 1, 2, 3, 4, and 5 min.…”

Section: Extraction (Vortex) Timementioning

confidence: 99%

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Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Pasupuleti

Tsai

Lin

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Organophosphate pesticides (OPPs) are the most commonly used insecticides around the world in various agricultural and domestic practices, and humans are frequently exposed to these hazardous insecticides that can lead to several chronic health effects. Therefore, a fast and sensitive analytical method is required for biomonitoring the markers of OPPs in humans for exposure estimation. In this study, a fast and sensitive analytical procedure was developed for the determination of the metabolites of OPPs in human urine samples. Methods Metabolites of OPPs were extracted from 2 mL of urine sample using a novel vortex‐assisted salt‐induced liquid–liquid microextraction (VA‐SI‐LLME) technique, and the preconcentrated metabolites were analyzed by ultra‐high‐performance liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS). Various factors affecting the efficiency of VA‐SI‐LLME were thoroughly investigated. Results The metabolites of OPPs exhibited very good linearity over the concentration range between 0.05 and 50 ng mL−1 with coefficient (r2) values ranging between 0.9986 and 0.9999. The method showed excellent sensitivity with detection limits ranging from 0.01 to 0.03 ng mL−1 and quantification limits from 0.03 to 0.05 ng mL−1. The developed method was applied to the analysis of real samples and the recoveries ranged between 85.0 and 114.1% with related standard deviations <5%. Conclusions The results showed the VA‐SI‐LLME/UHPLC/MS/MS method to be a simple, rapid, sensitive, and selective analytical procedure for the biomonitoring of the metabolites of OPPs in humans. This efficient and cost‐effective analytical method could be a potential alternative method for the biomonitoring of the metabolites of pesticides in humans.

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Section: Resultsmentioning

confidence: 99%

Section: Extraction (Vortex) Timementioning

confidence: 99%

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Pasupuleti

Tsai

Lin

et al. 2020

Rapid Comm Mass Spectrometry

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“…Among all, cotton leaf curl virus and R. solani severely affect the productivity and accounts for up to 40% reduction in yield [5,6]. The infection and infestation of plants lead to activation of protein inhibitors and induction of defense mechanisms including the release of secondary metabolites like phenolic compounds [7,8]. Phenylalanine and some phenolic compounds are derived from phosphoenol phosphate and erythrose-4-phosphate through the shikimate pathway.…”

Section: Introductionmentioning

confidence: 99%

“…Currently, several methodologies are in use for the analysis of phenolic compounds in plant systems. Amongst them, spectrophotometric-based Folin-Denis and differential pH assays are preferably used for quantification of total phenolics and anthocyanins, respectively [8]. However, these methods lack the specificity as well as selectivity which lead to over or underestimation of phenolic content [9].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

2019

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Plant secondary metabolites including phenolics and flavonoidsare synthesized through phenylpropanoid and phenylpropanoid-acetate pathways and significantly contribute against adverse effect of abiotic and biotic stresses. Herein, we present the development and execution of a novel and expeditious ultra-performance liquid chromatographic-photodiode array (UPLC-PDA) method for qualitative and quantitative analysis of 28 phenolic compounds comprising of flavonoids, phenolic acids, aldehydes and alcohols. The method is able to separate phenolic compounds in just 17 min with the separation of isobaric species such as 3,4 dihydroxybenzoic acid and 3,5 dihydroxy benzoic acid; quercetin and taxifolin. Linear curves concentrations ranged from 6-18 µg/mL (3,5 dihydroxy benzoic acid), 4-12 µg/mL (catechin and salicylic acid) and 2-6 µg/mL for rest of the compounds and correlation coefficients were >0.994. The limit of detection (LOD) varied from 0.04-0.45 µg/mL. Cotton root samples were used to assess the method in terms of recovery efficiency (85-120%), precision (0.12-4.09%) and intermediate precision (0.32-4.0%).Phenolics and flavonoidsin root samples of healthy and diseased plants as well as leaf samples of healthy plants were successfully quantified using this novel method without an expensive Mass Spectrometer.

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“…For example, LLE requires a large number of toxic solvents and is time-consuming and the SPE materials are expensive. In recent years, the development of dispersive liquid-liquid microextraction (DLLME) can make up the deficiencies of LLE and SPE [ 14 , 15 ]. The principle of DLLME is to form a ternary solvent system using extraction solvent (organic solvent), dispersant (hydrophilic solvent) and sample aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Vortex-Assisted Dispersive Liquid-Liquid Microextraction for the Simultaneous Quantitation of Eleven Non-Anthocyanin Polyphenols in Commercial Blueberry Using the Multi-Objective Response Surface Methodology and Desirability Function Approach

Xue

et al. 2018

Molecules

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In the present study, 11 non-anthocyanin polyphenols, gallic acid, protocatechuate, vanillic acid, syringic acid, ferulic acid, quercetin, catechin, epicatechin, epigallocatechin gallate, gallocatechin gallate and epicatechin gallate—were firstly screened and identified from blueberries using an ultra performance liquid chromatography–time of flight mass spectrography (UPLC-TOF/MS) method. Then, a sample preparation method was developed based on vortex-assisted dispersive liquid-liquid microextraction. The microextraction conditions, including the amount of ethyl acetate, the amount of acetonitrile and the solution pH, were optimized through the multi-objective response surface methodology and desirability function approach. Finally, an ultra performance liquid chromatography–triple quadrupole mass spectrography (UPLC-QqQ/MS) method was developed to determine the 11 non-anthocyanin polyphenols in 25 commercial blueberry samples from Sichuan province and Chongqing city. The results show that this new method with high accuracy, good precision and simple operation characteristics, can be used to determine non-anthocyanin polyphenols in blueberries and is expected to be used in the analysis of other fruits and vegetables.

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Vortex-assisted liquid-liquid–liquid microextraction followed by high performance liquid chromatography for the simultaneous determination of fourteen phenolic acids in honey, iced tea and canned coffee drinks

Cited by 44 publications

References 42 publications

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Rapid and sensitive analytical procedure for biomonitoring of organophosphate pesticide metabolites in human urine samples using a vortex‐assisted salt‐induced liquid–liquid microextraction technique coupled with ultra‐high‐performance liquid chromatography/tandem mass spectrometry

Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

Optimization of Vortex-Assisted Dispersive Liquid-Liquid Microextraction for the Simultaneous Quantitation of Eleven Non-Anthocyanin Polyphenols in Commercial Blueberry Using the Multi-Objective Response Surface Methodology and Desirability Function Approach

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