2010
DOI: 10.1186/1742-4690-7-1
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Vpu serine 52 dependent counteraction of tetherin is required for HIV-1 replication in macrophages, but not in ex vivo human lymphoid tissue

Abstract: BackgroundThe human immunodeficiency virus type 1 (HIV-1) Vpu protein degrades CD4 and counteracts a restriction factor termed tetherin (CD317; Bst-2) to enhance virion release. It has been suggested that both functions can be genetically separated by mutation of a serine residue at position 52. However, recent data suggest that the S52 phosphorylation site is also important for the ability of Vpu to counteract tetherin. To clarify this issue, we performed a comprehensive analysis of HIV-1 with a mutated casei… Show more

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Cited by 99 publications
(115 citation statements)
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“…HIV-1 virus stocks were generated by standard calcium phosphate transfection of 293T cells essentially as described previously (36). For infection of macrophages, Jurkat-LTRG R5 cells, or CemM7 T cells, we quantified HIV-1 stocks by p24 enzyme-linked immunosorbent assay (ELISA) (37) and incubated the cells for 6 h with the indicated amounts of p24. Cells were subsequently washed and cultured with the appropriate medium.…”
Section: Methodsmentioning
confidence: 99%
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“…HIV-1 virus stocks were generated by standard calcium phosphate transfection of 293T cells essentially as described previously (36). For infection of macrophages, Jurkat-LTRG R5 cells, or CemM7 T cells, we quantified HIV-1 stocks by p24 enzyme-linked immunosorbent assay (ELISA) (37) and incubated the cells for 6 h with the indicated amounts of p24. Cells were subsequently washed and cultured with the appropriate medium.…”
Section: Methodsmentioning
confidence: 99%
“…To assess viral spread and replication, aliquots of the infected cell culture supernatants were taken in two-day intervals and stored at Ϫ20°C. Virus production was analyzed by the quantification of p24 in the supernatants (37). Infectivity assays were done by infection of Jurkat-LTRG R5 cells with the indicated amounts of p24.…”
Section: Methodsmentioning
confidence: 99%
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“…ϩ T cell lines (41,61), primary CD4 ϩ T cells (41,(61)(62)(63), monocyte-derived macrophages (59,62,63,66), in vitro tonsil histocultures (59), and HeLa cells (12). However, Vpu was dispensable for HIV-1 virion production in cell lines such as 293T cells and HT1080 cells.…”
Section: Wt]) Hiv-1-infected/transfected Cells In Certain Cd4mentioning
confidence: 99%