Warmth Prevents Bone Loss Through the Gut Microbiota

Chevalier, Claire; Kieser, Silas; Çolakoğlu, Melis; Hadadi, Noushin; Brun, Julia; Rigo, Dorothée; Suárez-Zamorano, Nicolas; Spiljar, Martina; Fabbiano, Salvatore; Busse, Björn; Ivanišević, Julijana; Macpherson, Andrew J.; Bonnet, Nicolas; Trajkovski, Mirko

doi:10.1016/j.cmet.2020.08.012

Cited by 121 publications

(103 citation statements)

References 81 publications

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“…They also elevated in the proband’s mother (II3), and the level of OC increased remarkably in the aunt (II7). The variable level of bone formation markers in the three affected patients might be associated with the characterization of clinical heterogeneity or some factors, such as age, lifestyle, and other environmental stimulus ( Lundberg et al, 2018 ; Chevalier et al, 2020 ). Taken together, the upregulated or upper limit range of serum bone formation markers implied that the bone formation increased.…”

Section: Discussionmentioning

confidence: 99%

A Novel Variant in CLCN7 Regulates the Coupling of Angiogenesis and Osteogenesis

Peng

Wen

2020

Front. Cell Dev. Biol.

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Autosomal dominant osteopetrosis type II (ADO II), characterized by increased bone mass and density, is caused by mutations in the chloride channel 7 ( CLCN7 ) gene. In this study, a novel missense variant in CLCN7 (c.1678A > G; p.Met560Val) was identified in three symptomatic subjects and one carrier of a Chinese family with ADO II. Notably, bone formation markers, including osteocalcin and total procollagen type N-terminal propeptide, have increased or presented at the upper limit of the normal range in the three patients. Serum factors secreted by osteoclast lineage cells and affecting the CD31 hi EMCN hi vessel formation, such as tartrate-resistant acid phosphatase 5b, platelet-derived growth factor-BB, vascular endothelial growth factor, and SLIT3, had a higher expression in three ADO II subjects than in 15 healthy age-matched and sex-matched controls. Moreover, the conditioned medium was obtained from preosteoclast induced from the ADO II patients’ peripheral blood mononuclear cells. It was found to promote the CD31 hi EMCN hi vessel formation of human microvascular endothelial cells and osteogenic differentiation of bone marrow-derived stem cells. Taken together, our finding revealed a novel CLCN7 variant associated with ADO II and suggested that the sclerotic bone was potentially associated with the increase of the CD31 hi EMCN hi vessel formation and bone formation.

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Section: Discussionmentioning

confidence: 99%

A Novel Variant in CLCN7 Regulates the Coupling of Angiogenesis and Osteogenesis

Peng

Wen

2020

Front. Cell Dev. Biol.

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“…Please refer to the flowchart ( Figure 1 ) to define what experimental design will be suitable for your specific question. For a practical example of using these protocols, please refer to ( Chevalier et al., 2020 ). For osteoblasts, different validation tests are proposed in this protocol to assess different functions, each requiring a certain number of cells.…”

Section: Before You Beginmentioning

confidence: 99%

“…For the osteoblasts, we exemplify assessing their activity by measuring RNA expression of some key markers. A list of targets can be found in Chevalier et al. 2020 , and these include Collagen type 1 ( Col1a ), Osteopontin ( Opn ) or Osteocalcin ( Ocn ).…”

Section: Expected Outcomesmentioning

confidence: 99%

“…For the osteoclasts, we describe assessing their activity and differentiation by measuring RNA expression of several key markers. The list of potential targets can be found in Chevalier et al. 2020 , and these include Matrix metalloprotease 9 ( Mmp9 ), Cathepsin K ( Ctsk ) and Acid phosphatase 5, tartrate resistant ( Trap5b ).…”

Section: Expected Outcomesmentioning

confidence: 99%

“…We describe methods for addressing their activity and/or differentiation, enabling studying the effects of various treatments during or following differentiation ex vivo . For further practical example of using these protocols, please refer to Chevalier et al. (2020) .…”

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Primary mouse osteoblast and osteoclast culturing and analysis

et al. 2021

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Summary Mesenchymal-derived osteoblasts play a key role in bone formation via synthesis and mineralization of the bone and bone remodeling. Osteoclasts are multinucleated cells of hematopoietic origin with a role in bone resorption. Here, we describe a protocol for generating primary cultures of these two cell types from bone tissue including the femur, tibia, and humerus of young mice. We describe methods for addressing their activity and/or differentiation, enabling studying the effects of various treatments during or following differentiation ex vivo . For further practical example of using these protocols, please refer to Chevalier et al. (2020) .

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Self‐Mineralizing Dnazyme Hydrogel as a Multifaceted Bone Microenvironment Amendment for Promoting Osteogenesis in Osteoporosis

Huang

Zhu

et al. 2023

Adv Funct Materials

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The accumulation of reactive oxygen species (ROS) and minimal osteogenic raw material in the osteoporotic bone microenvironment greatly inhibits the activity of osteoblasts. Herein, it is originally proposed to construct a biomatrix multifaceted bone microenvironment amendment ‐Mineralized zippered G4‐Hemin DNAzyme hydrogel (MDH)‐to improve osteoporotic osteogenic capacity and promote high‐quality bone defect repair. The programmed design of the rolling circle amplified DNA hydrogel synthesis system allows the introduction of massive amounts of zippered G4‐Hemin DNAzyme in MDH. The zippered G4‐Hemin DNAzyme highly mimics the tight catalytic configuration of horseradish peroxidase and exerts excellent enzyme‐like activity with considerable ROS molecule scavenging ability. In addition, the DNA amplification by‐product pyrophosphate is ingeniously employed as a sufficient phosphorus source, thus constituting an autonomous mineralization system for waste reuse through the introduction of pyrophosphate hydrolase and calcium ions, which deposits in MDH as an osteogenic raw material and addresses the challenge of DNA hydrogel bio‐application stability. The remarkable in vitro and in vivo outcomes demonstrate that MDH can effectively improve the oxidative stress status of osteoblasts, restore the balance of mitochondrial membrane potential, and reduce apoptosis, ultimately demonstrating superior osteogenic capacity.

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Warmth Prevents Bone Loss Through the Gut Microbiota

Cited by 121 publications

References 81 publications

A Novel Variant in CLCN7 Regulates the Coupling of Angiogenesis and Osteogenesis

A Novel Variant in CLCN7 Regulates the Coupling of Angiogenesis and Osteogenesis

Primary mouse osteoblast and osteoclast culturing and analysis

Self‐Mineralizing Dnazyme Hydrogel as a Multifaceted Bone Microenvironment Amendment for Promoting Osteogenesis in Osteoporosis

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