Wasp venom peptide improves the proapoptotic activity of alendronate sodium in A549 lung cancer cells

Abstract

Background Lung cancer in men and women is considered the leading cause for cancer-related mortality worldwide. Anti-cancer peptides represent a potential untapped reservoir of effective cancer therapy. Methodology Box-Behnken response surface design was applied for formulating Alendronate sodium (ALS)-mastoparan peptide (MP) nanoconjugates using Design-Expert software. The optimization process aimed at minimizing the size of the prepared ALS-MP nanoconjugates. ALS-MP nanoconjugates’ particle size, encapsulation efficiency and the release profile were determined. Cytotoxicity, cell cycle, annexin V staining and caspase 3 analyses on A549 cells were carried out for the optimized formula. Results The results revealed that the optimized formula was of 134.91±5.1 nm particle size. The novel ALS-MP demonstrated the lowest IC50 (1.3 ± 0.34 μM) in comparison to ALS-Raw (37.6 ± 1.79 μM). Thus, the results indicated that when optimized ALS-MP nanoconjugate was used, the IC50 of ALS was also reduced by half. Cell cycle analysis demonstrated a significantly higher percentage of cells in the G2-M phase following the treatment with optimized ALS-MP nanoconjugates. Conclusion The optimized ALS-MP formula had significantly improved the parameters related to the cytotoxic activity towards A549 cells, compared to control, MP and ALS-Raw.

Andrographolide nanophytosomes exhibit enhanced cellular delivery and pro-apoptotic activities in HepG2 liver cancer cells

“…In addition, the highly linear correlation of the predicted versus observed size reflects the harmony between the observed and predicted values indicating the appropriateness of the model (Fahmy, Badr-Eldin et al., 2020 ). The experimental design results showed that sonication time in the current study exhibited a similar negative effect on the particle size, indicating that the particle size significantly decreases with increasing sonication time (Alhakamy et al., 2022 ). The observed reduction in size could be attributed to the main principle of the sonication process.…”

Natural Bacterial and Fungal Peptides as a Promising Treatment to Defeat Lung Cancer Cells

“…The results showed a lower IC 50 value (1.3 ± 0.34 µM) compared to ALS (IC 50 37.6 ± 1.79 µM). Moreover, after treatment with the ALS-MP nanoconjugates, a higher percentage of cells in the G2-M phase was noted during cell cycle analysis [30]. Cisplatin is used as a standard and effective drug in lung cancer chemotherapy.…”