Waste office paper: A potential feedstock for cellulase production by a novel strain Bacillus velezensis ASN1

Nair, Anu Sadasivan; Al-Battashi, Huda; Al-Akzawi, Ahlam; Annamalai, Neelamegam; Gujarathi, Ashish M.; Al-Bahry, Saif N.; Dhillon, Gurpreet Singh; Sivakumar, Nallusamy

doi:10.1016/j.wasman.2018.08.014

Cited by 46 publications

(24 citation statements)

References 51 publications

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“…Here in this study, we have focused on the higher enzyme cultivation study, rather than going for the mechanism involved behind the same. A study by Nair et al (2018) successfully optimized the cellulase activity to 2.42 U/ml in B. velezensis ASN1. A similar kind of study was done by Sivakumar et al (2016) to isolate and characterize B. licheniformis from compost.…”

Section: Enzyme Cellulase Activity On Wheat Strawmentioning

confidence: 99%

Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

et al. 2019

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In the present study, 11 cellulose degrading bacterial strains were isolated from water and soil samples of hot springs in the Chumathang village, Leh and Ladakh region, India. The isolated strains were identified as Bacillus subtilis, Bacillus aryabhattai, Bacillus stratosphericus, Bacillus altitudinis, and Brevibacterium frigoritolerans by biochemical and molecular approaches. All the strains were evaluated for the total cellulase, endoglucanase, exoglucanase, and β-glucosidase enzyme activities. On the basis of overall individual cellulose degrading enzyme activities, three strains were selected to develop consortium to enhance their cellulase enzyme activities. The potent cellulose degraders B. stratosphericus BHUJPV-H5, B. subtilis BHUJPV-H19, and B. subtilis BHUJPV-H12 were selected for the consortium development. The effect of cellulase activities of bacterial strains were evaluated ranged up to 6.06 and 0.72 mg ml −1 glucose by agro-residues of sugarcane bagasse and wheat straw, respectively, after 1 h of incubation. Total cellulase enzyme activity of consortia was recorded two times higher than the individual organisms. These strains can be used for enhancing bioethanol production from lignocellulosic biomass, which can consequently boost biofuel production industry. HIGHLIGHTS-Bacillus strains were isolated from hot spring's water and soil samples.-Total cellulase enzyme activity was two times higher in the form of consortium.β-glucosidase activity was found to be high, with maximum 5.65 Uml −1 .-Use of wheat straw and sugarcane bagasse showed good amounts of glucose release on hydrolysis.

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Section: Enzyme Cellulase Activity On Wheat Strawmentioning

confidence: 99%

Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

et al. 2019

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“…Nair et al . 27 isolated B. velezensis ASN1 that could synthesize cellulase used in food, textile, animal feed, petroleum, waste management, biosurfactant, and pulp/paper industries.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, it has been shown that B. velezensis strain 157 is able to degrade various agro-industrial byproducts including soybean meal, wheat bran, sugarcane bagasse, wheat straw, rice husk, maize flour and maize straw utilized in biofuel production. B. velezensis strains were also investigated for their ability to depolymerize various types of lignocelluloses into fermentable sugars 27 . Nair et al 27 isolated B. velezensis ASN1 that could synthesize cellulase used in food, textile, animal feed, petroleum, waste management, biosurfactant, and pulp/paper industries.…”

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Novel research on nanocellulose production by a marine Bacillus velezensis strain SMR: a comparative study

Abouelkheir

Kamara

Atia

et al. 2020

Sci Rep

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Bacterial nanocellulose (BNC) is a nanofibrillar polymer that possesses unique characteristics such as high chemical purity, mechanical strength, flexibility, and absorbency. In addition, different bacterial strains can form nanocellulose (NC) in multiple shapes and sizes. This study describes the first report of a marine Bacillus strain that is able to synthesize NC. The strain identified as B. velezensis SMR based on 16S rDNA sequencing, produced highly structured NC, as confirmed by X-ray diffraction (XRD) and Scanning Electron Microscopic Analysis (SEM). In Hestrin-Schramm (HS) medium, B. velezensis SMR produced twice the quantity of BNC in comparison to the reference strain, G. xylinus ATCC 10245. The ability of B. velezensis SMR to produce NC using different industrial waste materials as growth media was tested. Growth in Ulva seaweed extract supported a 2.5-fold increase of NC production by B. velezensis SMR and a threefold increase in nc production by G. xylinus ATCC 10245. As proof of principle for the usability of nc from B. velezensis SMR, we successfully fabricated a BNCbased polyvinyl alcohol hydrogel (BNC-PVA) system, a promising material used in different fields of application such as medicine, food, and agriculture. Bacterial nanocellulose (BNC), an extracellular produced structure, is considered a highly desirable biomaterial due to its superior qualities in comparison to other cellulose-containing structures. Compared to plant cellulose, nanofibril network of biocellulose possesses high water retaining capacity, degree of polymerization, chemical purity, high crystallinity, in vivo biocompatibility to be used as a scaffold in tissue engineering, and excellent mechanical properties 1,2. BNC production has been reported in both Gram-negative bacteria such as G. xylinus, Agrobacterium, Achromobacter, Aerobacter, Azotobacter, Pseudomonas, and Rhizobium, and Gram-positive bacteria such as Sarcina 3. Among the different BNC-producing bacteria, G. xylinus is the most commonly studied species 4. Synthesis of BNC is a complex process involving polymerization of glucose monomers and secretion of the complex cellulose structures to the external environment to create a three-dimensional microfibril and nanofibril network. During the fermentation process, bacteria either move freely in the media or attach to cellulose fibers, producing a highly swollen gel structure 5. Purification of NC from the culture medium involves the removal of bacterial cells and collection of the cellulose matrix from the cultural medium. This is a crucial step to ensure the quality of BNC and can be performed either by repeated washing using a hot sodium hydroxide solution, followed by water until reaching a neutral pH or by other methods, such as gamma radiation 6. While BNC-production by several bacterial species have been reported, it has never been shown that members of the genus Bacillus were able to produce NC. B. velezensis is a Gram-positive bacterium that has been extensively studied for its ability to induce plant growth-...

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“…Some lignocellulosic byproducts have been used for the production of BSs. The use of different biomasses as raw materials for the production of hemicellulosic hydrolysates for BS generation is described in the literature, such as trimming vine shoots (Bustos et al, 2005), barley bran, corncobs and Eucalyptus globulus (Moldes et al, 2006), distilled grape marc (Portilla-Rivera et al, 2007), sweet sorghum bagasse hydrolysate (Samad et al, 2014), orange peel (Kumar et al, 2016), sugarcane bagasse (Franco-Marcelino et al, 2017;Marcelino et al, 2019), waste office paper (Nair et al, 2018;Nair et al, 2020), and xylose−rich corncob hydrolysate (Chen et al, 2019).…”

Section: Biosurfactantsmentioning

confidence: 99%

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

et al. 2020

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The integrated approach in biorefinery mainly involves the utilization of various agroindustrial byproducts such as raw materials for the production of several biobased products like biofuels, bioenergy, and other high-value chemicals. Biofuels are the backbone of biorefineries, however, production of value-added biomolecules such as biopigments, biopolymers, biosurfactants, and nutritional yeast has been attracting great attention. The production of these biomolecules using traditional approaches has been extensively studied in the last few years owing to their promising application in different industries such as chemical, food/feed, and pharmaceuticals for the development of novel products for mankind. Moreover, the production of such biomolecules using lignocellulosic, starchy, and some other agroindustrial byproducts is still not fully explored. Hence, there is a huge scope in the development of sustainable biorefining approaches to make the technology cost-effective. The lignocellulosic biomasses usually used in biorefineries are mainly composed of cellulose, hemicellulose, and lignin, whereas starchy materials, besides starch, usually contain, protein, lipids, and some micronutrients. The processing of these biomasses through successive steps like pretreatments, enzymatic hydrolysis, and fermentation is essentially required to obtained final biobased products. Considering certain bottlenecks of above-mentioned conventional biorefineries approaches, new technologies have been proposed for the improved pretreatment of biomass and efficient enzymatic hydrolysis in order to minimize the concentration of toxic inhibitors in resulting hydrolysate. In this review, we highlighted the different agroindustrial byproducts and their applications for the production of valuable biorefinery products.

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Waste office paper: A potential feedstock for cellulase production by a novel strain Bacillus velezensis ASN1

Cited by 46 publications

References 51 publications

Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

Developing Efficient Thermophilic Cellulose Degrading Consortium for Glucose Production From Different Agro-Residues

Novel research on nanocellulose production by a marine Bacillus velezensis strain SMR: a comparative study

Agroindustrial Byproducts for the Generation of Biobased Products: Alternatives for Sustainable Biorefineries

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