Waste Polyurethane Foams as Biomass Carriers in the Treatment Process of Domestic Sewage with Increased Ammonium Nitrogen Content

Abstract

In order to understand the mechanisms of microbial growth on waste polyurethane sponge materials, their effectiveness as biomass carriers in domestic sewage with increased ammonium nitrogen content treatment was assessed. Comparative experiments were carried out in microreactors under steady conditions of batch culture, which allowed for an assessment of different carriers, in the form of flexible foams, rigid foams, and flexible foams placed in full casings. In the studies conducted in continuous cultures, biomass carriers selected in batch culture were used as fillings in the column model. The structure of the microbial community inhabiting the spongy material was determined and the pollutant-removing process from real domestic sewage was assessed. Analyzes using the Illumina sequencing technique allowed for demonstrating that Nitrosomonas and Nitrospira were the predominant nitrifiers in the biomass carrier in the form of waste polyurethane foams (PUF). It was found that anammox bacteria, the presence of which—as unidentified Planctomycetes—was confirmed in the polyurethane sponge material, were also responsible for the high removal of N-NH4+. Burkholderia and Sphingopyxis phyla were identified as the dominant denitrifying bacteria involved in the treatment of domestic sewage with increased content of ammonium nitrogen. The biomass carrier in the form of waste PUF placed additionally in full casings proved to be more beneficial for the proliferation of bacteria involved in nitrification and denitrification processes. On the other hand, waste foams without casings proved to be more suitable for the growth of microorganisms known to perform partial denitrification and may accumulate nitrites (Staphylococcus, Dokdonella). Additionally, the presence of Devosia and Pseudonocardia, which participated in the phosphorus removal process, was found in the waste PUR foams.