Wastewater based epidemiology as a silent sentinel of the trend of SARS-CoV-2 circulation in the community in central Argentina

Masachessi, Gisela; Castro, Gonzalo; Cachi, Ariana M.; Marinzalda, María de los Angeles; Liendo, Matías; Pisano, María Belén; Sicilia, Paola; Ibarra, Gustavo; Rojas, Ricardo Manuel; López, Laura Muñoz; Barbás, Gabriela; Cardozo, Diego; Ré, Viviana Elisabeth; Nates, Silvia

doi:10.1016/j.watres.2022.118541

Cited by 17 publications

(10 citation statements)

References 57 publications

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“…Indeed, for such countries, wastewater epidemiology may be the only feasible means of effective population surveillance ( 9 ). Similar successful experiences have been reported in the Americas regions, such as in other areas of Argentina ( 23 , 28 , 30 , 33 , 34 ), Brazil ( 35 ), and Peru ( 16 ).…”

Section: Discussionsupporting

confidence: 80%

“…The difficulties imposed by the COVID-19 pandemic did not give enough time to standardize the protocols for wastewater testing, so each region had to adapt the monitoring method based on its health infrastructure and economy. Among the different concentration methods for wastewater effluent for the detection of pathogens, the most used methods were based on ultrafiltration ( 29 ), precipitation ( 30 ), and flocculation ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

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Presence of SARS-CoV-2 in urban effluents in south-east Buenos Aires, Argentina, May 2020 to March 2022

Cimmino,

Rodrigues Capítulo,

Lerman

et al. 2023

Revista Panamericana De Salud Pública

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Objectives. To implement and evaluate the use of wastewater sampling for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in two coastal districts of Buenos Aires Province, Argentina. Methods. In General Pueyrredon district, 400 mL of wastewater samples were taken with an automatic sampler for 24 hours, while in Pinamar district, 20 L in total (2.2 L at 20-minute intervals) were taken. Samples were collected once a week. The samples were concentrated based on flocculation using polyaluminum chloride. RNA purification and target gene amplification and detection were performed using reverse transcription polymerase chain reaction for clinical diagnosis of human nasopharyngeal swabs. Results. In both districts, the presence of SARS-CoV-2 was detected in wastewater. In General Pueyrredon, SARS-CoV-2 was detected in epidemiological week 28, 2020, which was 20 days before the start of an increase in coronavirus virus disease 2019 (COVID-19) cases in the first wave (epidemiological week 31) and 9 weeks before the maximum number of laboratory-confirmed COVID-19 cases was recorded. In Pinamar district, the virus genome was detected in epidemiological week 51, 2020 but it was not possible to carry out the sampling again until epidemiological week 4, 2022, when viral circulation was again detected. Conclusions. It was possible to detect SARS-CoV-2 virus genome in wastewater, demonstrating the usefulness of the application of wastewater epidemiology for long-term SARS-CoV-2 detection and monitoring.

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Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Presence of SARS-CoV-2 in urban effluents in south-east Buenos Aires, Argentina, May 2020 to March 2022

Cimmino,

Rodrigues Capítulo,

Lerman

et al. 2023

Revista Panamericana De Salud Pública

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“…This anticipation demonstrates the great potential of genomic surveillance in wastewater samples. Ideally, for best WBE, variant detection and viral load monitoring in wastewater over time should be investigated in a complementary way (Galani et al 2022 ; Johnson et al 2022 ; Masachessi et al 2022 ; Rubio-Acero et al 2021 ). Few studies reported similar lead times (Joshi et al 2022 ; Vo et al 2022 ) while other achieved shorter lead times, around 7 days (Kirby et al 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Wastewater early warning system for SARS-CoV-2 outbreaks and variants in a Coruña, Spain

Trigo-Tasende

Vallejo

Rumbo‐Feal

et al. 2023

Environ Sci Pollut Res

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Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at the community level. Here we describe COVIDBENS, a wastewater surveillance program running from June 2020 to March 2022 in the wastewater treatment plant of Bens in A Coruña (Spain). The main goal of this work was to provide an effective early warning tool based in wastewater epidemiology to help in decision-making at both the social and public health levels. RT-qPCR procedures and Illumina sequencing were used to weekly monitor the viral load and to detect SARS-CoV-2 mutations in wastewater, respectively. In addition, own statistical models were applied to estimate the real number of infected people and the frequency of each emerging variant circulating in the community, which considerable improved the surveillance strategy. Our analysis detected 6 viral load waves in A Coruña with concentrations between 103 and 106 SARS-CoV-2 RNA copies/L. Our system was able to anticipate community outbreaks during the pandemic with 8–36 days in advance with respect to clinical reports and, to detect the emergence of new SARS-CoV-2 variants in A Coruña such as Alpha (B.1.1.7), Delta (B.1.617.2), and Omicron (B.1.1.529 and BA.2) in wastewater with 42, 30, and 27 days, respectively, before the health system did. Data generated here helped local authorities and health managers to give a faster and more efficient response to the pandemic situation, and also allowed important industrial companies to adapt their production to each situation. The wastewater-based epidemiology program developed in our metropolitan area of A Coruña (Spain) during the SARS-CoV-2 pandemic served as a powerful early warning system combining statistical models with mutations and viral load monitoring in wastewater over time.

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“…The present article is part of a federal research program aimed at examining wastewater to evaluate the circulation of the SARS-CoV-2 virus in Argentina during the pandemic. The investigations provided epidemiological data concerning different provinces, Buenos Aires (Barrios et al, 2021; Iglesias et al, 2021), Santa Fé (Reno et al, 2022), Mendoza (Giraud-Billoud, et al, 2021), Córdoba (Masachessi et al, 2022), Salta (Cruz et al, 2023; Maidana-Kulesza, et al, 2022). It is the first surveillance report carried out in the Province of Tucumán to develop a strategy for detecting SARS-CoV-2 at the city level from wastewater.…”

Section: Discussionmentioning

confidence: 99%

Wastewater monitoring of SARS-CoV-2 gene for COVID-19 epidemiological surveillance in Tucumán Argentina

D’Arpino,

Sineli,

Goroso

et al. 2023

Preprint

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Epidemiology based on the detection of pathogens in wastewater is extremely useful in providing information about a population’s health status. This study aimed to analyze and report the epidemiological dynamics of SARS-CoV-2 in the province of Tucumán, Argentina during the second and third surges of COVID-19 between April 2021 and March 2022. The study aimed to quantify SARS-CoV-2 RNA in wastewater, correlating it with clinically reported COVID-19 cases. Wastewater samples (n=72) were collected from 16 sampling points located in 3 cities of Tucumán (San Miguel de Tucumán, Yerba Buena y Banda del Río Salí). Detection of viral nucleocapsid markers (N1 gene) was carried out using one-step RT-qPCR. Viral loads were determined for each positive sample using a standard curve. A positive correlation (p<0.05) was observed between viral load (copies/mL) and the clinically confirmed COVID-19 cases reported during the sampling period in San Miguel de Tucumán. Our research findings provided a crucial insight into the dynamics of SARS-CoV-2 infection during epidemic outbreaks. The implementation of wastewater monitoring has proven to be an invaluable epidemiological tool, facilitating early detection of potential surges in COVID-19 cases, and enabling a comprehensive tracking of the pandemic. Our study underscores the significance of incorporating SARS-CoV-2 genome-based surveillance as a standard practice which will contribute to anticipating any future spikes in infections.

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Wastewater based epidemiology as a silent sentinel of the trend of SARS-CoV-2 circulation in the community in central Argentina

Cited by 17 publications

References 57 publications

Presence of SARS-CoV-2 in urban effluents in south-east Buenos Aires, Argentina, May 2020 to March 2022

Presence of SARS-CoV-2 in urban effluents in south-east Buenos Aires, Argentina, May 2020 to March 2022

Wastewater early warning system for SARS-CoV-2 outbreaks and variants in a Coruña, Spain

Wastewater monitoring of SARS-CoV-2 gene for COVID-19 epidemiological surveillance in Tucumán Argentina

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