Water-Based Extraction of Bioactive Principles from Blackcurrant Leaves and Chrysanthellum americanum: A Comparative Study

Abstract

The water-based extraction of bioactive components from flavonoid-rich medicinal plants is a key step that should be better investigated. This is especially true when dealing with easy-to-use home-made conditions of extractions, which are known to be a bottleneck in the course for a better control and optimization of the daily uptake of active components from medicinal plants. In this work, the water-based extraction of Blackcurrant (Ribes nigrum) leaves (BC) and Chrysanthellum americanum (CA), known to have complementary pharmacological properties, was studied and compared with a previous work performed on the extraction of Hawthorn (Crataegus, HAW). Various extraction modes in water (infusion, percolation, maceration, ultrasounds, microwaves) were compared for the extraction of bioactive principles contained in BC and CA in terms of extraction yield, of amount of flavonoids, phenolic compounds, and proanthocyanidin oligomers, and of UHPLC profiles of the extracted compounds. The qualitative and quantitative aspects of the extraction, in addition to the kinetic of extraction, were studied. The optimized easy-to-use-at-home extraction protocol developed for HAW was found very efficient to easily extract bioactive components from BC and CA plants. UHPLC-ESI-MS and high-resolution Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) were also implemented to get more qualitative information on the specific and common chemical compositions of the three plants (including HAW). Their antihyaluronidase, antioxidant, and antihypertensive activities were also determined and compared, demonstrating similar activities as the reference compound for some of these plants.

Cytotoxic Properties on Prostate and Cervical Cancer Cells in Culture of «<i>Acti-plus</i>», a Recipe Based on Medicinal Plants from Burkina Faso

“…Several studies have shown that the medicinal plants that make up this recipe follow these trends. Cao-Ngoc et al, showed that the aqueous extract of Chrysanthelllum americanum DC was mainly composed of flavonoids (flavanones, flavones and aurones) and phenolic acids (hydroxycinnamic acid) [22]. In a study conducted in Burkina Faso, Guenne et al, evaluated the antioxidant capacity and antilipoxygenase potential of Chrysanthelllum americanum DC.…”

Screening and Evaluation of Dermo-Cosmetic Activities of the Invasive Plant Species Polygonum cuspidatum

“…The effects of P. cuspidatum extracts and four analytical standards (polydatin, emodin, resveratrol and chlorogenic acid) were evaluated towards hyaluronidase catalytic activity using an offline CE-based assay optimized in a previous study [ 97 ]. CE assays were carried out with some adjustment considering the presence of EtOH in plant extracts as well as in standards and its concentration was limited to 5% of the final volume.…”

Homogenate-Ultrasound-Assisted Ionic Liquid Extraction of Total Flavonoids from Selaginella involven: Process Optimization, Composition Identification, and Antioxidant Activity

“…According to the report of Cao-Ngoc with some modifications, the raw materials (15.0 g) were moistened with 180 mL of 95% ethanol and swelled in a closed container for 3 h. Then, the swollen material was transferred into the percolator, and the appropriate amount of 95% ethanol was added to extract for 24 h. After that, the first concentrated extract was collected. Extraction was continued until the constituents were completely exhausted.…”