Water content, transition temperature and fragility influence protection and anhydrobiotic capacity

Ramirez, John F.; Kumara, U.G.V.S.S.; Arulsamy, Navamoney; Boothby, Thomas C.

doi:10.1016/j.bbadva.2024.100115

Cited by 6 publications

(9 citation statements)

References 82 publications

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“…T g is the temperature at which a glassy material will begin to transition into a more rubbery phase. Previous work has established that even small amounts of additives, such as glycerol, can lead to changes in the T g of a vitrifying material, which can correlate with changes to protective capacity (Weng and Elliott, 2015;Ramirez et al, 2024). With this in mind, we were curious if the T g differs between sucrose-glycerol mixtures composed of different sized sucrose species.…”

Section: Glass Transition Temperature Correlates With the Protective ...mentioning

confidence: 99%

“…Previous work has identified several properties of vitrified systems that are linked to their protective capacity during desiccation (Crowe et al, 1998;Ballesteros and Walters, 2019;Ramirez et al, 2024). The first of these is the glass transition temperature (T g ), the temperature at which a vitrified material transitions from a glass-like state to a more rubbery phase (Angell, 1995;Ito et al, 1999;Grasmeijer et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Conversely, a fragile glass former would not increase in viscosity dramatically in the initial stages of drying, but rather would increase in viscosity only close to the point at which it vitrifies (Angell, 1995;Ballesteros and Walters, 2019). Glass former fragility has been mostly studied in the context of cryogenic vitrification, but has been seen to correlate with protection in a limited number of studies involving dry vitrification (Walters, 2004;Ballesteros and Walters, 2019;Ramirez et al, 2024).…”

Section: Introductionmentioning

confidence: 99%

“…Water content is an additional property of a vitrified material that has been considered with regard to glassy properties that could influence protection (Ramirez et al, 2024). Water within a vitrified system has been shown to be protective in and of itself, providing hydrogen bonds that can stabilize proteins and maintain membrane organization (Wright, 1989;Hibshman et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…In a recent report, the properties of vitrified systems that promote protection were investigated using a series of mixtures comprising one of three different disaccharides (trehalose, maltose, and sucrose) and increasing amounts of glycerol (Ramirez et al, 2024). It showed that the glassy property(s); glass former fragility, glass transition temperature, and water content associated with protection varied from disaccharide to disaccharide.…”

Section: Introductionmentioning

confidence: 99%

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The effect of sucrose polymer-size on glass transition temperature, glass former fragility, and water retention during drying

Kumara,

Ramirez,

Boothby

2024

Front. Mater.

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Water is essential for all active life processes. Despite this, there are a number of organisms that can survive prolonged desiccation. The vitrification hypothesis posits that such organisms survive desiccation by forming non-crystalline amorphous (vitrified) solids, often through the accumulation of protective disaccharides. In line with this theory, vitrification has been shown to be essential for desiccation tolerance in many organisms that survive extreme drying. However, it is known that not all vitrified materials are protective and that certain physio-chemical properties correlate with the protection in the glassy state. Furthermore, recent evidence suggests that the physio-chemical properties that correlate with protection can vary depending on the chemical nature of similarly sized protectants. While the chemistry of protectants has been probed in relation to the protective properties they induce when vitrified, the effect of protectant size on glassy properties and protection during drying has not been investigated. Here, we study the effect of the polymer size of sucrose on glassy properties associated with protection in the vitrified state. The monomer sucrose, and the polymers polysucrose 70 and polysucrose 400 (70 and 400 refer to the molecular weight of the polymers in kDa). Using these three different-sized sucrose polymers, we find that each of the glassy properties we investigated including; enzyme protection, water content, glass transition temperature, and glass former fragility, were affected by polymer size. However, only one vitrified property, glass transition temperature, correlated with protection during drying. This correlation is heavily dependent on sucrose polymer size. Increased glass transition midpoint temperature correlated positively with protection conferred by monomeric sucrose (p-value = 0.009, R2 = 0.840), whereas this correlation was bi-phasic for polysucrose 70, and had an inverse relationship for polysucrose 400 (p-value = 0.120, R2 = 0.490). Our results indicate that the size of vitrifying protectants can have a profound effect on glassy properties as well as on how these properties correlate with protection in the dry state. Beyond desiccation tolerance, these findings provide insights for the development of new technologies for the stabilization of biological material in the dry state.

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Section: Glass Transition Temperature Correlates With the Protective ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The effect of sucrose polymer-size on glass transition temperature, glass former fragility, and water retention during drying

Kumara,

Ramirez,

Boothby

2024

Front. Mater.

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Disordered proteins interact with the chemical environment to tune their protective function during drying

KC,

Nguyen,

Nicholson

et al. 2024

Preprint

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The conformational ensemble and function of intrinsically disordered proteins (IDPs) are sensitive to their solution environment. The inherent malleability of disordered proteins combined with the exposure of their residues accounts for this sensitivity. One context in which IDPs play important roles that is concomitant with massive changes to the intracellular environment is during desiccation (extreme drying). The ability of organisms to survive desiccation has long been linked to the accumulation of high levels of cosolutes such as trehalose or sucrose as well as the enrichment of IDPs, such as late embryogenesis abundant (LEA) proteins or cytoplasmic abundant heat soluble (CAHS) proteins. Despite knowing that IDPs play important roles and are co-enriched alongside endogenous, species-specific cosolutes during desiccation, little is known mechanistically about how IDP-cosolute interactions influence desiccation tolerance. Here, we test the notion that the protective function of desiccation-related IDPs is enhanced through conformational changes induced by endogenous cosolutes. We find that desiccation-related IDPs derived from four different organisms spanning two LEA protein families and the CAHS protein family, synergize best with endogenous cosolutes during drying to promote desiccation protection. Yet the structural parameters of protective IDPs do not correlate with synergy for either CAHS or LEA proteins. We further demonstrate that for CAHS, but not LEA proteins, synergy is related to self-assembly and the formation of a gel. Our results demonstrate that functional synergy between IDPs and endogenous cosolutes is a convergent desiccation protection strategy seen among different IDP families and organisms, yet, the mechanisms underlying this synergy differ between IDP families.

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Enhancing Physicochemical, Bioactive, and Nutritional Properties of Sweet Potatoes: Ultrasonic Contact Drying with Slot Jet Nozzles Compared to Hot-Air and Freeze-Drying

Yildiz,

Gao,

Ding

et al. 2024

Preprint

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Water content, transition temperature and fragility influence protection and anhydrobiotic capacity

Cited by 6 publications

References 82 publications

The effect of sucrose polymer-size on glass transition temperature, glass former fragility, and water retention during drying

The effect of sucrose polymer-size on glass transition temperature, glass former fragility, and water retention during drying

Disordered proteins interact with the chemical environment to tune their protective function during drying

Enhancing Physicochemical, Bioactive, and Nutritional Properties of Sweet Potatoes: Ultrasonic Contact Drying with Slot Jet Nozzles Compared to Hot-Air and Freeze-Drying

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