Water Extract from Spent Mushroom Substrate of Hericium erinaceus Suppresses Bacterial Wilt Disease of Tomato

Abstract

Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding β-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.

Combination of agricultural waste compost and biofertilizer improves yield and enhances the sustainability of a pepper field

“…), especially 150 d after treatment. This enhancement of disease control can be partially due to an additive effect as both beneficial microbe and SMS are effective in disease control ( Liu et al., ; Kwak et al., ). Moreover, the enhanced biocontrol efficiency was also possibly attributed to synergistic disease suppression acquired with a rapid and long‐lasting beneficial bacterial growth and colonization supported by the enriched nutrition and appropriate humidity from the agro‐industry waste in the BF+organic fertilizers combination.…”

Oxalic Acid from Lentinula edodes Culture Filtrate: Antimicrobial Activity on Phytopathogenic Bacteria and Qualitative and Quantitative Analyses

“…As shown in Fig. 1 , the culture filtrate exhibited antibacterial activity, forming a clear zone of 22 mm on the paper disk method [ 9 ] against Ralstonia solanacearum , a causal agent of tomato bacterial wilt disease. To purify the antibacterial compound, the culture filtrate was subjected to octadecyl-silica column chromatography and eluted with different concentrations from 5% to 100% methanol.…”

“…Commercial organic acid (Sigma, St. Louis, MO, USA) was tested for its antibacterial activity against R. solanacearum using the paper disk method [ 9 ]. Different oxalic acid concentrations (100 to 5,000mg/L) were added to the filter paper disks (5 mm diameter), placed on nutrient agar, and overlaid with soft agar (0.8%) mixed with bacterial cells (5 × 10 6 ).…”

Comparative transcriptome analysis of dikaryotic mycelia and mature fruiting bodies in the edible mushroom Lentinula edodes

“…Among Korean L . edodes cultivars, the dikaryotic strain Sanjo 701 (SJ701) in particular is popular for sawdust-bag cultivation and has been important in the development of monokaryotic strains for mating and fruiting 4 and antibacterial substances against different phytopathogenic bacteria 5 .…”

“…Mushroom enzymes with various functional properties, including antibiotic, antioxidant, and antitumor activities, have been extracted from cell culture filtrates as well as mycelia and fruiting bodies 5 . Because L .…”