Water Quality and Total Microbial Load: A Double-Threshold Identification Procedure Intended for Space Applications

Amalfitano, Stefano; Levantesi, Caterina; Garrelly, Laurent; Giacosa, D.; Bersani, Francesca; Rossetti, Simona

doi:10.3389/fmicb.2018.02903

Cited by 9 publications

(5 citation statements)

References 64 publications

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“…Conversely, we found that AMR‐cap enrichment has the opposite effect on samples obtained from wastewater treatment plants. Wastewater samples are notorious for having a high microbial load and, in comparison, to other sample types have higher levels of ARGs (Amalfitano et al ., 2018). The poultry litter samples represent a middle ground between the built environment and wastewater samples, as some samples and resistance genes are sequenced at deeper levels with AMR‐cap enrichment.…”

Section: Discussionmentioning

confidence: 99%

Escaping the fate of Sisyphus: assessing resistome hybridization baits for antimicrobial resistance gene capture

Beaudry

Thomas

Baptista

et al. 2021

Environmental Microbiology

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Finding, characterizing and monitoring reservoirs for antimicrobial resistance (AMR) is vital to protecting public health. Hybridization capture baits are an accurate, sensitive and cost-effective technique used to enrich and characterize DNA sequences of interest, including antimicrobial resistance genes (ARGs), in complex environmental samples. We demonstrate the continued utility of a set of 19 933 hybridization capture baits designed from the Comprehensive Antibiotic Resistance Database (CARD)v1.1.2 and Pathogenicity Island Database (PAIDB)v2.0, targeting 3565 unique nucleotide sequences that confer resistance. We demonstrate the efficiency of our bait set on a custommade resistance mock community and complex environmental samples to increase the proportion of ontarget reads as much as >200-fold. However, keeping pace with newly discovered ARGs poses a challenge when studying AMR, because novel ARGs are continually being identified and would not be included in bait sets designed prior to discovery. We provide imperative information on how our bait set performs against CARDv3.3.1, as well as a generalizable approach for deciding when and how to update hybridization capture bait sets. This research encapsulates the full life cycle of baits for hybridization capture of the resistome from design and validation (both in silico and in vitro) to utilization and forecasting updates and retirement.

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Section: Discussionmentioning

confidence: 99%

Escaping the fate of Sisyphus: assessing resistome hybridization baits for antimicrobial resistance gene capture

Beaudry

Thomas

Baptista

et al. 2021

Environmental Microbiology

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“…It is suggested that pathogenic microorganism having the smallest size in water be determined before selection of a proper membrane for cold pasteurization or sterilization of water. Countermeasures for contaminations by "unexpected" pathogens may also be important for avoiding health risks in consumers (Amalfitano et al, 2018 ). This aspect has also be highlighted by other studies (Orhan et al, 2021 ; Beck et al, 2021 ).…”

Section: Scientific Basis Of Membrane Filtration To Remove Microorgan...mentioning

confidence: 99%

Application of Membrane Filtration to Cold Sterilization of Drinks and Establishment of Aseptic Workshop

2023

Food Environ Virol

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Aseptic packaging of high quality beverage is necessary and its cold-pasteurization or sterilization is vital. Studies on application of ultrafiltration or microfiltration membrane to cold- pasteurization or sterilization for the aseptic packaging of beverages have been reviewed. Designing and manufacturing ultrafiltration or microfiltration membrane systems for cold-pasteurization or sterilization of beverage are based on the understanding of size of microorganisms and theoretical achievement of filtration. It is concluded that adaptability of membrane filtration, especially its combination with other safe cold method, to cold- pasteurization and sterilization for the aseptic packaging of beverages should be assured without a shadow of doubt in future.

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“…Some drawbacks of the method are partly circumvented by using external and internal standards and by operating under controlled reproducible settings. Therefore, the correlation between intracellular ATP content and microbial cell counts will rely on a robust cross-calibration with results from other reference methods (Amalfitano et al, 2018b;Hammes et al, 2010). It has been calculated that the average ATP-per-cell content is approximately 1.75 x 10 -10 nmol/cell or 6.87 x 10 -17 g ATP/cell (K. .…”

Section: Sequencing-based "-Omics" Approaches For Microbial Communitymentioning

confidence: 99%

“…The mature technologies, herein entitled for water microbial monitoring in space, showed their own critical advantages and limitations (table 1). Both current and future applications in space will be necessarily bounded by the definition of novel standards of microbial quality (i.e., other than those applied on Earth by cultivation-dependent approaches) (Amalfitano et al, 2018b), but also by a compromise between the best analytical performances and detrimental technical issues to cope with during spaceflights.…”

Section: Comparative Analysis Of Space-relevant Technological Featuresmentioning

confidence: 99%

“…The microgravity conditions provide conflicting results on microbial growth and virulence of opportunistic human pathogens (Morrison et al, 2017;, while the analysis of archival samples generates a detrimental time gap for a flight-supportive result interpretation (Huang et al, 2018;Novikova et al, 2006). HPC was proved to be also affected by carbon sources in cultivation media, the incubation time, and the initial microbial load level (Amalfitano et al, 2018b). Finally, the prompt development and requirement update of alternate bio-analytical technologies for microbial monitoring during space 9 exploration is pushed fundamentally by the need to avoid microbial regrowth from analytical wastes (Wong et al, 2017).…”

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confidence: 99%

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Water and Microbial Monitoring Technologies towards the Near Future Space Exploration

Amalfitano¹,

Levantesi²,

Copetti³

et al. 2019

Preprint

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Space exploration is demanding longer lasting human missions and water resupply from Earth will become increasingly unrealistic. In a near future, the spacecraft water monitoring systems will require technological advances to promptly identify and counteract contingent events of waterborne microbial contamination, posing health risks to astronauts with lowered immune responsiveness. The search for bio-analytical approaches, alternative to those applied on Earth by cultivation-dependent methods, is pushed by the compelling need to limit waste disposal and avoid microbial regrowth from analytical carryovers. Prospective technologies will be selected only if first validated in a flight-like environment, by following basic principles, advantages, and limitations beyond their current applications on Earth. Starting from the water monitoring activities applied on the International Space Station, we provide a critical overview of the nucleic acid amplification-based approaches (i.e., loop-mediated isothermal amplification, quantitative PCR, and high-throughput sequencing) and early-warning methods for total microbial load assessments (i.e., ATP-metry, flow cytometry), already used at a high readiness level aboard crewed space vehicles. Our findings suggest that the forthcoming space applications of mature technologies will be necessarily bounded by a compromise between analytical performances (e.g., speed to results, identification depth, reproducibility, multiparametricity) and detrimental technical requirements (e.g., reagent usage, waste production, operator skills, crew time). As space exploration progresses toward extended missions to Moon and Mars, miniaturized systems that also minimize crew involvement in their end-to-end operation are likely applicable on the long-term and suitable for the in-flight water and microbiological research.

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Water Quality and Total Microbial Load: A Double-Threshold Identification Procedure Intended for Space Applications

Cited by 9 publications

References 64 publications

Escaping the fate of Sisyphus: assessing resistome hybridization baits for antimicrobial resistance gene capture

Escaping the fate of Sisyphus: assessing resistome hybridization baits for antimicrobial resistance gene capture

Application of Membrane Filtration to Cold Sterilization of Drinks and Establishment of Aseptic Workshop

Water and Microbial Monitoring Technologies towards the Near Future Space Exploration

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