Water Soluble Mixed Ligand Complexes Spectral, antioxidant, antimicrobial and DNA interaction studies

Abstract

A new series of water soluble mixed ligand complexes [MII(L)(bpy)AcO].nH2O M = Cu, (n=1) (1); Co (2), Mn (3), Ni (4) (n=4) and Zn (5) (n=2) have been synthesized from 2-(2-Morpholinoethylimino)methyl)phenol Schiff base ligand (HL) and 2,2�-bipyridine in a 1:1:1 molar ratio. The resulting complexes were characterized by spectral techniques. The spectral data of these complexes suggest an octahedral geometry. In vitro antioxidant activity results of DPPH assay, hydroxyl radical, super oxide and nitric oxide for complexes (1-5) were compared with ligand (HL) and in vitro antimicrobial activities of all compounds were examined against selected bacterial and fungal strains which indicate that the complexes exhibit higher antimicrobial activity than free ligand (HL). Gel electrophoresis results indicated that, complexes (1) and (2) have exhibited more DNA cleavage efficiency than others. The intrinsic binding constant (Kb) values for the complexes (1-5) observed by electronic absorption technique were in the order of (1) ] (4) ] (5) ] (2) ] (3) and DNA binding affinity values for these complexes obtained by viscosity measurements were in the order of ethidium bromide ] (1) ] (2) ] (3) ] (4) ] (5).

An Integrated Analysis of Mechanistic Insights into Biomolecular Interactions and Molecular Dynamics of Bio-Inspired Cu(II) and Zn(II) Complexes towards DNA/BSA/SARS-CoV-2 3CLpro by Molecular Docking-Based Virtual Screening and FRET Detection

“…We carefully monitored the absorbance at 517, 230, 590, and 546 nm while carrying out the antioxidant properties for DPPH, hydroxyl, superoxide, and nitric oxide radical scavenging. In addition, the obtained IC 50 findings of all test substances were also contrasted with those of standard ascorbic acid [ 40 , 41 , 42 ].…”

Exploring the antioxidant, antimicrobial, cytotoxic and biothermodynamic properties of novel morpholine derivative bioactive Mn(ii), Co(ii) and Ni(ii) complexes – combined experimental and theoretical measurements towards DNA/BSA/SARS-CoV-2 3CLPro

“…These methods are frequently employed to evaluate a compound's ability to scavenge free radicals and the antioxidant activity of the targeted free ligand and metal complexes. [119][120][121][122] The observed percentage of inhibition efficiency for all substances in terms of the IC 50 findings for these assays is shown in Fig. S42a-d and Tables S12-S15.…”

Comprehensive Assessment of Biomolecular Interactions of Morpholine‐Based Mixed Ligand Cu(II) and Zn(II) Complexes of 2,2′‐Bipyridine as Potential Anticancer and SARS‐CoV‐2 Agents: A Synergistic Experimental and Structure‐Based Virtual Screening

“…While performing the antioxidant properties for the DPPH, hydroxyl, superoxide, and nitric oxide radical scavenging, the absorbance at 517, 230, 590, and 546 nm, respectively, was closely observed. In addition, the observed IC 50 values of all samples were compared with ascorbic acid [49][50][51].…”