Water soluble molybdenocene complexes: Synthesis, cytotoxic activity and binding studies to ubiquitin by fluorescence spectroscopy, circular dichroism and molecular modeling

Narváez-Pita, Xiomara; Ortega-Zúñiga, Carlos; Acevedo-Morantes, Claudia Y.; Pastrana, Belinda; Olívero-Verbel, Jesús; Maldonado-Rojas, Wilson; Ramı́rez-Vick, Jaime E.; Meléndez, Enrique

doi:10.1016/j.jinorgbio.2013.10.014

Cited by 10 publications

(17 citation statements)

References 58 publications

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“…It can be inferred that the titled complexes bind to CT-DNA with an intercalative mode [18]. The quenching of EB bound to DNA with the observed linearity in the plot is in good agreement with the linear Stern-Volmer equation [19] given below:…”

Section: Fluorescence Spectrasupporting

confidence: 81%

Copper(II) Complexes Based on Aminohydroxamic Acids: Synthesis, Structures, In Vitro Cytotoxicities and DNA/BSA Interactions

Zhang

Yang

et al. 2018

Crystals

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(4) (glyha 2− = dianion glycinehydroxamic acid, alaha 2− = dianion alaninehydroxamic acid, bpy = 2,2 -bipyridine, phen = 1,10-phenanthroline) have been successfully synthesized and characterized by X-ray single crystal diffraction. The interactions of these complexes with calf thymus DNA (CT-DNA) were studied through UV spectroscopy, fluorescence spectroscopy, and circular dichroism. The results revealed that complexes 1-4 could interact with CT-DNA through intercalation. Interactions of all complexes with bovine serum albumin (BSA) were confirmed by the docking study to quench the intrinsic fluorescence of BSA in a static quenching process. Furthermore, the in vitro cytotoxic effect of the complexes was also examined on four tumor cell lines, including human lung carcinoma cell line (A549), human colon carcinoma cell line (HCT-116), human promyelocytic leukemia cell (HL-60) and cervical cancer cell line (HeLa). All complexes exhibited different antitumor activities.

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Section: Fluorescence Spectrasupporting

confidence: 81%

Copper(II) Complexes Based on Aminohydroxamic Acids: Synthesis, Structures, In Vitro Cytotoxicities and DNA/BSA Interactions

Zhang

Yang

et al. 2018

Crystals

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“…Finally, although IC 50 values for 2 were about an order of magnitude higher than the state‐of‐the‐art anti‐cancerous drugs, the concept of a drug that initially is luminescent (and thus can be employed as an imaging and/or PDT agent), but consequently is capable to convert into a cytostatic moiety could be an interesting and novel approach to the development of theranostic and/or multimodal therapeutic agents. In this respect, octahedral cluster complexes may be of particular interest due to their photoluminescence combined with the ability to hydrolyse and to bind strongly to a range of donor groups –…”

Section: Figurementioning

confidence: 99%

From Photoinduced to Dark Cytotoxicity through an Octahedral Cluster Hydrolysis

Svezhentseva

Vorotnikov

Solovieva

et al. 2018

Chemistry A European J

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Octahedral molybdenum and tungsten clusters have potential biological applications in photodynamic therapy and bioimaging. However, poor solubility and hydrolysis stability of these compounds hinder their application. The first water-soluble photoluminescent octahedral tungsten cluster [{W I }(DMSO) ](NO ) was synthesised and demonstrated to be at least one order of magnitude more stable towards hydrolysis than its molybdenum analogue. Biological studies of the compound on larynx carcinoma cells suggest that it has a significant photoinduced toxicity, while the dark toxicity increases with the increase of the degree of hydrolysis. The increase of the dark toxicity is associated with the in situ generation of nanoparticles that clog up the cisternae of rough endoplasmic reticulum.

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“…In recent times, the studies on DNA interactions and antiproliferative activities of molybdenum complexes have gained substantial interest. − However, structural reports on oxidomolybdenum complexes with salen type ligands are few − and their pharmacological properties remain practically unexplored.…”

Section: Introductionmentioning

confidence: 99%

Monomeric and Dimeric Oxidomolybdenum(V and VI) Complexes, Cytotoxicity, and DNA Interaction Studies: Molybdenum Assisted C═N Bond Cleavage of Salophen Ligands

et al. 2017

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Four novel dimeric bis-μ-imido bridged metal-metal bonded oxidomolybdenum(V) complexes [MoOL'] (1-4) (where L' are rearranged ligands formed in situ from HL) and a new mononuclear dioxidomolybdenum(VI) complex [MoOL] (5) synthesized from salen type NO ligands are reported. This rare series of imido-bridged complexes (1-4) have been furnished from rearranged HL' ligands, containing an aromatic diimine (o-phenylenediamine) "linker", where Mo assisted hydrolysis followed by -C═N bond cleavage of one of the arms of the ligand HL took place. A monomeric molybdenum(V) intermediate species [MoO(HL')(OEt)] (I) was generated in situ. The concomitant deprotonation and dimerization of two molybdenum(V) intermediate species (I) ultimately resulted in the formation of a bis-μ-imido bridge between the two molybdenum centers of [MoOL'] (1-4). The mechanism of formation of 1-4 has been discussed, and one of the rare intermediate monomeric molybdenum(V) species I has been isolated in the solid state and characterized. The monomeric dioxidomolybdenum(VI) complex [MoOL] (5) was prepared from the ligand HL where the aromatic "linker" was replaced by an aliphatic diimine (1,2-diaminopropane). All the ligands and complexes have been characterized by elemental analysis, IR, UV-vis spectroscopy, NMR, ESI-MS, and cyclic voltammetry, and the structural features of 1, 2, 4, and 5 have been solved by X-ray crystallography. The DNA binding and cleavage activity of 1-5 have been explored. The complexes interact with CT-DNA by the groove binding mode, and the binding constants range between 10 and 10 M. Fairly good photoinduced cleavage of pUC19 supercoiled plasmid DNA was exhibited by all the complexes, with 4 showing the most promising photoinduced DNA cleavage activity of ∼93%. Moreover, in vitro cytotoxic activity of all the complexes was evaluated by MTT assay, which reveals that the complexes induce cell death in MCF-7 (human breast adenocarcinoma) and HCT-15 (colon cancer) cell lines.

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Water soluble molybdenocene complexes: Synthesis, cytotoxic activity and binding studies to ubiquitin by fluorescence spectroscopy, circular dichroism and molecular modeling

Cited by 10 publications

References 58 publications

Copper(II) Complexes Based on Aminohydroxamic Acids: Synthesis, Structures, In Vitro Cytotoxicities and DNA/BSA Interactions

Copper(II) Complexes Based on Aminohydroxamic Acids: Synthesis, Structures, In Vitro Cytotoxicities and DNA/BSA Interactions

From Photoinduced to Dark Cytotoxicity through an Octahedral Cluster Hydrolysis

Monomeric and Dimeric Oxidomolybdenum(V and VI) Complexes, Cytotoxicity, and DNA Interaction Studies: Molybdenum Assisted C═N Bond Cleavage of Salophen Ligands

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