WAVE2 regulates meiotic spindle stability, peripheral positioning and polar body emission in mouse oocytes

Sun, Shao‐Chen; Xu, Yong‐Nan; Li, Yinghua; Lee, Seung‐Eun; Jin, Yong-Xun; Cui, Xiang‐Shun; Kim, Nam‐Hyung

doi:10.4161/cc.10.11.15796

Cited by 44 publications

(45 citation statements)

References 36 publications

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“…This pattern is similar to that of actin and confirms observations from previous studies in somatic cells and germ cells (Welch et al 1997;Sun et al 2011). The Arp2/3 complex is an actin nucleator, and actin plays a critical role in contractile ring formation, cytokinesis and after cell division (Pollard & Cooper 2009).…”

Section: Discussionsupporting

confidence: 80%

“…Two of these proteins, Arp2 and Arp3, are related to actin and serve as the first two subunits of the nascent actin filament when the Arp2/3 complex is activated (Rouiller et al 2008). Various nucleation-promoting factors (NPFs) including N-WASP (Yi et al 2011), WAVE2 (Sun et al 2011), and junction-mediating and regulatory protein (JMY) bind to and activate the Arp2/ 3 complex in response to Rho family GTPase signaling. These NPFs are essential components that mediate dynamic actin reorganization during oocyte maturation .…”

Section: Introductionmentioning

confidence: 99%

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Inhibition of the Arp2/3 complex impairs early embryo development of porcine parthenotes

Lin

et al. 2016

Animal Cells and Systems

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The Arp2/3 complex, which nucleates actin filaments, comprises a stable assembly of seven-protein subunits including two actin-related proteins (Arp2 and Arp3). Previous work showed that Arp2/3 binds to the sides of actin filaments and is concentrated at the leading edges of motile cells. In the present study, we show that the Arp2/3 complex is critical for cytokinesis during early embryonic development in porcine parthenotes. The Arp2/3 complex is concentrated at the cortex of each cell at the 1-, 2-, and 4-cell stages, and at the periphery at the morula stage. The amount of Arp2/3 significantly decreased at the blastocyst stage in parthenogenetically activated porcine embryos. Inhibition of the Arp2/3 complex in the pig embryos by the Arp2/3-specific inhibitor CK666 resulted in abnormal cell division, a decrease in developmental rate and total cell numbers, and an increase in the ratio of trophectoderm cell number to inner cell mass number in blastocyst-stage embryos. In addition, 4-cell stage embryos subjected to CK666 treatment exhibited significantly decreased expression of ZGA genes (Pou5f1, Sox2, and Nanog), suggesting that the Arp2/3 complex plays an important role in early porcine embryo development. Thus, our data demonstrate that the Arp2/3 complex is required for early embryonic development in pigs and appears to regulate the expression of pluripotency genes. ARTICLE HISTORY

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Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Inhibition of the Arp2/3 complex impairs early embryo development of porcine parthenotes

Lin

et al. 2016

Animal Cells and Systems

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“…The roles of actin polymerization and various actin nucleators in spindle migration and asymmetric cell division in maturing oocytes have recently emerged 7,[27][28][29][30][31][32][33] ; however, the roles of other actin-binding proteins in oocyte maturation, including actin-capping proteins, ADF/cofilin, and tropomyosins, which are critical for the control of actin dynamics, are poorly understood. In this study, we present evidence that maintenance of cortical actin by Tpm3 is critical for spindle migration and polar body extrusion.…”

Section: Discussionmentioning

confidence: 99%

Non-muscle tropomyosin (Tpm3) is crucial for asymmetric cell division and maintenance of cortical integrity in mouse oocytes

Jang

Jo²,

Kim³

et al. 2014

Cell Cycle

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“…Actin nucleators initiate new actin filaments (Firat-Karalar and Welch, 2011), and several of these proteins are essential for oocyte maturation, including formin-2 (FMN2) (Dumont et al, 2007;Leader et al, 2002), Spire (Pfender et al, 2011) and the Arp2/3 complex (Chaigne et al, 2013;Sun et al, 2011b;Yi et al, 2011). Various nucleation-promoting factors, including JMY (Sun et al, 2011a), N-WASP (Yi et al, 2011) and WAVE2 (Chaigne et al, 2013;Sun et al, 2011c), activate the Arp2/3 complex and help to form new branched actin filaments. In addition to actin nucleators, other actin-binding proteins cap, depolymerize, elongate and bundle actin filaments, and are therefore crucial for actin dynamics in various cell types (Pollard and Cooper, 2009).…”

Section: Introductionmentioning

confidence: 99%

Actin-capping proteins play essential roles in asymmetric division of maturing mouse oocytes

Jang

Namgoong

et al. 2014

Journal of Cell Science

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Actin polymerization is essential for various stages of mammalian oocyte maturation, including spindle migration, actin cap formation, polar body extrusion and cytokinesis. The heterodimeric actincapping protein is an essential element of the actin cytoskeleton. It binds to the fast-growing (barbed) ends of actin filaments and plays essential roles in various actin-mediated cellular processes. However, the roles of capping protein in mammalian oocyte maturation are poorly understood. We investigated the roles of capping protein in mouse oocytes and found that it is essential for correct asymmetric spindle migration and polar body extrusion. Capping protein mainly localized in the cytoplasm during maturation. By knocking down or ectopically overexpressing this protein, we revealed that it is crucial for efficient spindle migration and maintenance of the cytoplasmic actin mesh density. Expression of the capping-protein-binding region of CARMIL (also known as LRRC16A) impaired spindle migration and polar body extrusion during oocyte maturation and decreased the density of the cytoplasmic actin mesh. Taken together, these findings show that capping protein is an essential component of the actin cytoskeleton machinery that plays crucial roles in oocyte maturation, presumably by controlling the cytoplasmic actin mesh density.

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WAVE2 regulates meiotic spindle stability, peripheral positioning and polar body emission in mouse oocytes

Cited by 44 publications

References 36 publications

Inhibition of the Arp2/3 complex impairs early embryo development of porcine parthenotes

Inhibition of the Arp2/3 complex impairs early embryo development of porcine parthenotes

Non-muscle tropomyosin (Tpm3) is crucial for asymmetric cell division and maintenance of cortical integrity in mouse oocytes

Actin-capping proteins play essential roles in asymmetric division of maturing mouse oocytes

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