Wavelength Dependence of Histological, Physical, and Visible Changes in Chronically Uv‐irradiated Hairless Mouse Skin*

Bissett, Donald; Hannon, Daniel P.; Orr, T. V.

doi:10.1111/j.1751-1097.1989.tb02907.x

Cited by 158 publications

(103 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…(B) The measured in vivo action spectrum for the photosagging of mouse skin taken from the results of Bissett and coworkers (reprinted with permission from ref. 40). The in vivo action spectrum mimics the action spectrum shown in A.…”

Section: Fig 2 (A)mentioning

confidence: 99%

“…These chromophores have been implicated in photoaging, but a quantitative comparison between their absorption spectra and photoaging action spectra has not been made. Additional action spectra (skin fold thickening, glycosaminoglycan production, collagen damage, cellularity increase, and elastosis in mice models) also reflect a UV-A component that have a similar line shape as the triplet state action spectrum of t-UA (40). It would not be surprising if the generation of O 2 ( 1 ⌬ g ) by energy transfer from the excited triplet state of t-UA in the UV-A also contributes to these physiological responses.…”

Section: Biophysics: Hanson and Simonmentioning

confidence: 99%

See 1 more Smart Citation

Epidermaltrans-urocanic acid and the UV-A-induced photoaging of the skin

Hanson

Simon

1998

Proc. Natl. Acad. Sci. U.S.A.

146

115

View full text Add to dashboard Cite

The premature photoaging of the skin is mediated by the sensitization of reactive oxygen species after absorption of ultraviolet radiation by endogenous chromophores. Yet identification of UV-A-absorbing chromophores in the skin that quantitatively account for the action spectra of the physiological responses of photoaging has remained elusive. This paper reports that the in vitro action spectrum for singlet oxygen generation after excitation of trans-urocanic acid mimics the in vivo UV-A action spectrum for the photosagging of mouse skin. The data presented provide evidence suggesting that the UV-A excitation of trans-urocanic acid initiates chemical processes that result in the photoaging of skin.Most of the visible signs of aging result from chronic exposure of the skin to ultraviolet radiation (1-2). Unlike chronologically aged skin that results from a general atrophy and a gradual decline in the production of the dermal matrix (3), UV-A (320-400 nm) photoaged skin is characterized by a gross increase in the elastic fibers (elastin, fibrillin, and desmosine) of the skin replacing the collagenated dermal matrix (elastosis) (4-5), an increase in glycosaminoglycans (4-5), collagen cross-linking, epidermal thickening (4-6), and an increase in the number of dermal cysts (7). The deep lines, leathered appearance, and the sagging of the skin surface typically associated with ''old age'' are thought to result from UV-induced photodamage to the skin and to occur over the course of a lifetime (3). Although obvious differences between photoaged and chronologically aged skin exist, the anatomic basis of the visible signs of photoaging is not understood fully (4, 8). Absorption of UV-A must induce photobiologic effects within the skin that lead to the visible and histological differences of photoaged skin, and, although the mechanisms by which UV-A-induced photodamage occur have not been completely determined, reactive oxygen species are postulated to play a role (9). The natural shift toward a more prooxidant state in chronologically aged skin then could be exacerbated by absorption of UV-A radiation by endogenous chromophores like NADH͞NADPH (10-14), tryptophan (15), and riboflavin (9), which then sensitize the formation of reactive oxygen species. Solar radiation has been shown both to reduce the antioxidant population in the skin (16) and to sensitize the production of reactive oxygen species such as singlet oxygen, hydrogen peroxide, and the superoxide anion (9-19), increasing the potential for reactions like the oxidation of lipids and proteins (17) that influence the degree of cross-linking between collagen and other proteins (9) within the skin.The first step toward identifying the chromophore(s) responsible for physiological changes, such as those seen in photoaged skin, is to match the action spectrum for the physiological change with the absorption spectrum of the chromophore (20). Once such a comparison is made, the pathways that lead to the physiological change can be unraveled. For example, the epid...

show abstract

Section: Fig 2 (A)mentioning

confidence: 99%

Section: Biophysics: Hanson and Simonmentioning

confidence: 99%

Epidermaltrans-urocanic acid and the UV-A-induced photoaging of the skin

Hanson

Simon

1998

Proc. Natl. Acad. Sci. U.S.A.

146

115

View full text Add to dashboard Cite

show abstract

“…Growth, differentiation and Figure 13. Action spectra for elastosis in hairless mice skin according to Bissett et al [227], Wulf et al [228] and Kligman and Sayre [229], for skin sagging in hairless mice skin according to Bissett et al [227] and for singlet oxygen generation after excitation of trans-urocanic in vitro (Hanson and Simon) [226]. Figure 14.…”

Section: Cataractmentioning

confidence: 99%

Solar radiation and human health

Moan

Juzeniene

2010

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

The Sun has played a major role in the development of life on Earth. In Western culture, people are warned against Sun exposure because of its adverse effects: erythema, photoimmunosuppression, photoageing, photocarcinogenesis, cataracts and photokeratitis. However, Sun exposure is also beneficial, since moderate doses give beneficial physiological effects: vitamin D synthesis, reduction of blood pressure and mental health. Shortage of Sun exposure may be even more dangerous to human health than excessive exposure. Avoiding Sun exposure leads to vitamin D deficiency which is associated not only with rickets and osteomalacia, but also with increased risk of cardiovascular disease, multiple sclerosis, rheumatoid arthritis, diabetes, influenza, many types of cancer and adverse pregnancy outcomes. Solar radiation induces nitric oxide release in tissue and immediate pigment darkening which certainly play important roles, although these are still unknown. Action spectra relevant for health are described. We will also review what is known about spectral and intensity variations of terrestrial solar radiation as well as its penetration through the atmosphere and into human skin and tissue.

show abstract

“…Bissett et al (1989) determined the wavelength dependence of several histological, physical and visible changes using narrow band width radiation as emitted by a xenon arc equipped with a dual grating monochrometer. Mice were exposed to the U V spectrum from 280-380 nm at 5-10 nm intervals.…”

Section: Action Spectramentioning

confidence: 99%

THE HAIRLESS MOUSE and PHOTOAGING

Kligman

1991

Photochem & Photobiology

View full text Add to dashboard Cite

Wavelength Dependence of Histological, Physical, and Visible Changes in Chronically Uv‐irradiated Hairless Mouse Skin*

Cited by 158 publications

References 15 publications

Epidermaltrans-urocanic acid and the UV-A-induced photoaging of the skin

Epidermaltrans-urocanic acid and the UV-A-induced photoaging of the skin

Solar radiation and human health

THE HAIRLESS MOUSE and PHOTOAGING

Contact Info

Product

Resources

About