Weaning Affects the Expression of Heat Shock Proteins in Different Regions of the Gastrointestinal Tract of Piglets

David, Jean‐Claude; Grongnet, Jean‐François; Lallès, Jean Paul

doi:10.1093/jn/132.9.2551

Cited by 39 publications

(45 citation statements)

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“…Furthermore, animal-to-animal variation and large interspecies variation have been found in Hsp70 expression in human, rat, and pig. This may be related to two functional promoter variants of hsp70 gene and the variation in stress susceptibility (David et al 2002;Khassaf et al 2001;Schwerin et al 2001). In the present study, we also observed that the hsp70 mRNA transcription varies with its corresponding Hsp70 protein expression in the same pattern.…”

Section: Discussionsupporting

confidence: 77%

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Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Zhong

Wang

Zhang

et al. 2010

Cell Stress and Chaperones

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The objective of this study is to investigate the expression and distribution of heat shock protein 70 (Hsp70) in the intestine of intrauterine growth retardation (IUGR) piglets. Samples from the duodenum, prejejunum, distal jejunum, ileum, and colon of IUGR and normalbody-weight (NBW) piglets were collected at birth. The results indicated that the body and intestine weight of IUGR piglets were significantly lower than NBW piglets. The villus height and villus/crypt ratio in jejunum and ileum of IUGR piglets were significantly reduced compared to NBW piglets. These results indicated that IUGR causes abnormal gastrointestinal morphologies and gastrointestinal dysfunction. The mRNA of hsp70 was increased in prejejunum (P<0.05), distal jejunum (P<0.05), and colon in IUGR piglets. However, the hsp70 mRNA in ileum of piglets with IUGR was decreased. Similar to hsp70 mRNA, the protein levels of Hsp70 in prejejunum (P<0.05), distal jejunum, and colon (P<0.05) in IUGR piglets were higher than those in NBW piglets. These results indicated that the expression of Hsp70 in the intestinal piglets was upregulated by IUGR, and different intestinal sites had different responses to stress. Meanwhile, the localization of Hsp70 in the epithelial cells of the whole villi and intestinal gland rather than in the lamina propria and myenteron suggested that Hsp70 has a cytoprotective role in epithelial cell function and structure.

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Section: Discussionsupporting

confidence: 77%

“…A similar variation has been found in the induction of Hsp70 in pig after transportation (Sepponen and Pösö 2006). In the gastrointestinal tract of pigs, weaning has been shown to cause abnormal spatial-temporal patterns of Hsp70 expression (David et al 2002). These imply that different intestinal sites had different responses to stress.…”

Section: Discussionsupporting

confidence: 64%

Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Zhong

Wang

Zhang

et al. 2010

Cell Stress and Chaperones

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“…Recently, HSP 25 and HSP 70 were demonstrated to be implicated in the protective actions of butyrate [70] and glutamine [88], respectively, in cultured cells. Since these nutrients are known to alleviate small intestine PW alterations in piglets, we studied the impact of weaning on gut HSP patterns [16]. Most changes observed in HSP protein concentrations were precocious (6 to 48 h PW) and transient (Tab.…”

Section: Weaning and Intestinal Cell Protection By Heat Shock Proteinsmentioning

confidence: 99%

“…Weaning induced an early drop, followed by an Table IV. Post-weaning changes in the tissue levels of heat shock proteins (HSP) in various sites of the gut of young pigs weaned at 28 days of age (adapted from [16]). [20].…”

Section: Weaning and Intestinal Mucinsmentioning

confidence: 99%

Gut function and dysfunction in young pigs: physiology

et al. 2004

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-The post-weaning period in pigs is characterized by an immediate but transient drop in feed intake resulting in severe undernutrition and growth check. This in turn affects various aspects of small intestinal architecture and function leading to gut-associated disorders and often diarrhea. Among these, villus atrophy and digestive enzyme activity depression have been documented. More recent investigations clearly demonstrate early signs of local inflammation including immune cell infiltration and increased pro-inflammatory cytokine gene expression, signs of cytoprotection through up-regulation of so-called heat shock proteins, indications of tissue alterations by proteases (stromelysin) and finally epithelial functional disorders in mineral absorption/secretion and permeability. This is followed by a regenerative phase, probably stimulated by feed intake resumption, resulting in down-regulation of many intestinal indicators. However, some of them then display new spatio-temporal adult-type adaptive patterns of maturation. A limited number of substances, particularly nitrogenous compounds and complex preparations of animal origin (colostrum, plasma) have proven to be successful, at least partly, in minimizing post-weaning intestinal disturbances. Thus further research in intestinal physiology, in association with microbiology and immunology, is warranted to strengthen our understanding of the mechanisms of gut disorders in order to provide a better rational basis for designing suitable alternatives to in-feed antibiotics for pigs. clairement montré des signes précoces d'inflammation incluant une infiltration cellulaire et l'expression accrue des gènes de plusieurs cytokines inflammatoires, une cytoprotection renforcée par la sur-expression des protéines du choc thermique, des indications d'altérations tissulaires par des protéases (stromélysines), et finalement des désordres fonctionnels épithéliaux d'absorption et de sécrétion minérales et de la perméabilité intestinale. Ceci est suivi par une phase de régénération intestinale, probablement stimulée par la reprise de consommation alimentaire, et conduisant à un retour à la normale de plusieurs indicateurs. Cependant, certains d'entre eux ont évolué vers des profils spatio-temporels adaptatifs de type adulte. Un nombre limité de substances, particulièrement des composés azotés et des produits animaux (colostrum, plasma) ont démontré leur capacité, au moins partielle, à minimiser les perturbations digestives post-sevrage. De nouvelles recherches en physiologie digestive, en association avec la microbiologie et l'immunologie, sont nécessaires pour renforcer notre compréhension des mécanismes des troubles digestifs. Ceci permettra de fournir des bases plus rationnelles pour développer des solutions alternatives satisfaisantes aux antibiotiques dans les aliments pour porcelets.intestin / nutrition / physiopathologie / porcelet / sevrage

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“…Par ailleurs, Lallès, 2010). la plupart des HSP sont réduites dans l'iléon après sevrage, ce qui pourrait contribuer à expliquer les altérations de barrière à ce niveau (David et al 2002). Collectivement, les données disponibles confirment l'importance du stress, du système nerveux et des mastocytes dans l'apparition des troubles digestifs du sevrage chez le porcelet.…”

Section: Données Récentesunclassified

Fonction de barrière et protection cellulaire de l’épithélium intestinal. Application au porcelet au sevrage

Lallès¹

2013

bavf

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Cette revue fait le point sur les récents progrès dans la compréhension de la fonction de barrière intestinale et de la cytoprotection épithéliale par les protéines du choc thermique (HSP). La régulation de la fonction de barrière et son importance pour l'espèce porcine sont présentées. Des progrès importants dans la compréhension de la fonction de barrière et de la cytoprotection ont été réalisés récem-ment. Les jonctions serrées et les HSP jouent un rôle majeur. Ces systèmes de protection sont hautement dynamiques, intégrés et finement régulés au niveau de l'épithélium intestinal. Le premier niveau de régulation est exercé par le microbiote. Le second niveau implique le système nerveux, le mastocyte et divers médiateurs agissant sur l'épithélium. La barrière est influencée par le stress ou par des perturbations du microbiote. Des études complémentaires méritent d'être conduites chez le porc pour définir des stratégies alimentaires et d'élevage destinées à limiter les perturbations digestives du sevrage. This review focuses on recent progress on the understanding of intestinal barrier function and epithelial cell protection by heat shock proteins (HSP). Barrier function regulation and its importance for

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Weaning Affects the Expression of Heat Shock Proteins in Different Regions of the Gastrointestinal Tract of Piglets

Cited by 39 publications

References 39 publications

Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Heat shock protein 70 is upregulated in the intestine of intrauterine growth retardation piglets

Gut function and dysfunction in young pigs: physiology

Fonction de barrière et protection cellulaire de l’épithélium intestinal. Application au porcelet au sevrage

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