Weight loss and abnormal lung inflammation in mice chronically exposed to secondary organic aerosols

Déméautis, Tanguy; Bouyssi, Alexandra; Géloën, Alain; George, Christian; Menotti, Jean; Gléhen, Olivier; Devouassoux, Gilles; Bentaher, Abderrazzak

doi:10.1039/d2em00423b

Cited by 3 publications

(10 citation statements)

References 48 publications

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“…The relative contribution of these immune cells to disease pathogenesis warrants investigation. Concomitant with such cell influx, our results revealed changes in the levels of a myriad of mediators in response to SOA . These molecules were either proinflammatory or anti-inflammatory mediators and their protein levels varied in the function of exposure time.…”

Section: Discussionmentioning

confidence: 64%

“…We, therefore, assessed the protein levels of multiple inflammatory mediators in response to SOA exposure using a cytokine antibody array approach. Our data found changes in the levels of the majority of mediators after exposure to SOA . In the present study, we focused on IL-6 and TNF-α, two well-studied inflammatory mediators in lung diseases.…”

Section: Resultsmentioning

confidence: 94%

“…Age-matched control mice received PBS containing DMSO at a final concentration of 0.2%. Mice (DMSO and SOA groups) were subjected to long-term exposure once per day for 5 days per week for 30 and 60 days . Following exposure periods, mice were sacrificed and their lungs lavaged.…”

Section: Methodsmentioning

confidence: 99%

“…The levels of various cytokines in cell-free BALs were examined using the RayBio mouse cytokine antibody array 6 (RayBiotech, Tebu-Bio, Le Perray-en-Yvelines, France), according to the manufacturer’s instructions …”

Section: Methodsmentioning

confidence: 99%

“…Based on the molecular weight markers, we identified MMP2 and MMP9. In support of this, our mediator inflammatory antibody microarray approach 40 confirmed the increased protein levels of these two MMPs.…”

Section: Ros Generationmentioning

confidence: 99%

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Chronic Exposure to Secondary Organic Aerosols Causes Lung Tissue Damage

Déméautis

Bouyssi

Chapalain

et al. 2023

Environ. Sci. Technol.

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Recently, secondary organic aerosols (SOAs) emerged as a predominant component of fine particulate matter. However, the pathogenic mechanism(s) of SOAs are still poorly understood. Herein, we show that chronic exposure of mice to SOAs resulted in lung inflammation and tissue destruction. Histological analyses found lung airspace enlargement associated with massive inflammatory cell recruitment predominated by macrophages. Concomitant with such cell influx, our results found changes in the levels of a series of inflammatory mediators in response to SOA. Interestingly, we observed that the expression of the genes encoding for TNF-α and IL-6 increased significantly after one month of exposure to SOAs; mediators that have been largely documented to play a role in chronic pulmonary inflammatory pathologies. Cell culture studies confirmed these in vivo findings. Of importance as well, our study indicates increased matrix metalloproteinase proteolytic activity suggesting its contribution to lung tissue inflammation and degradation. Our work represents the first in vivo study, which reports that chronic exposure to SOAs leads to lung inflammation and tissue injury. Thus, we hope that these data will foster new studies to enhance our understanding of the underlying pathogenic mechanisms of SOAs and perhaps help in the design of therapeutic strategies against SOA-mediated lung injury.

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Section: Discussionmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Ros Generationmentioning

confidence: 99%

See 3 more Smart Citations

Chronic Exposure to Secondary Organic Aerosols Causes Lung Tissue Damage

Déméautis

Bouyssi

Chapalain

et al. 2023

Environ. Sci. Technol.

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Toxicological Effects of Secondary Air Pollutants

Xiang

Wang

et al. 2023

Chem. Res. Chin. Univ.

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S econdary air pollutants, originating from gaseous pollutants and primary particulate matter emitted by natural sources and human activities, undergo complex atmospheric chemical reactions and multiphase processes. Secondary gaseous pollutants represented by ozone and secondary particulate matter, including sulfates, nitrates, ammonium salts, and secondary organic aerosols, are formed in the atmosphere, affecting air quality and human health. This paper summarizes the formation pathways and mechanisms of important atmospheric secondary pollutants. Meanwhile, different secondary pollutants’ toxicological effects and corresponding health risks are evaluated. Studies have shown that secondary pollutants are generally more toxic than primary ones. However, due to their diverse source and complex generation mechanism, the study of the toxicological effects of secondary pollutants is still in its early stages. Therefore, this paper first introduces the formation mechanism of secondary gaseous pollutants and focuses mainly on ozone’s toxicological effects. In terms of particulate matter, secondary inorganic and organic particulate matters are summarized separately, then the contribution and toxicological effects of secondary components formed from primary carbonaceous aerosols are discussed. Finally, secondary pollutants generated in the indoor environment are briefly introduced. Overall, a comprehensive review of secondary air pollutants may shed light on the future toxicological and health effects research of secondary air pollutants.

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Time‐Resolved Measurements of PM_2.5 Chemical Composition and Brown Carbon Absorption in Nanjing, East China: Diurnal Variations and Organic Tracer‐Based PMF Analysis

Feng,

Wang,

Shao

et al. 2023

JGR Atmospheres

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To understand diurnal variations in PM2.5 composition and aerosol extract absorption, PM2.5 samples were collected at intervals of 2 hr from 8:00 to 20:00 and 6 hr from 20:00 to 8:00 (the next day) in northern Nanjing, China, during the winter and summer of 2019–2020 and analyzed for bulk components, organic tracers, and light absorption of water and methanol extracts—a proxy measure of brown carbon (BrC). Diurnal patterns of measured species reflected the influences of primary emissions and atmospheric processes. Light absorption coefficients of water (Abs365,w) and methanol extracts (Abs365,m) at 365 nm shared a similar diurnal profile peaking at 18:00–20:00, generally following changes in biomass burning tracers. However, Abs365,w, Abs365,m, and their normalizations to organic aerosols increased at 14:00–16:00, earlier than that of levoglucosan in the late afternoon, which was attributed to secondarily formed BrC. The methanol extracts showed a less drastic decrease in light absorption at night than the water extracts and elevated absorption efficiency during 2:00–8:00. This is due to the fact that the water‐insoluble OC has a longer lifetime and stronger light absorption than the water‐soluble OC. According to the source apportionment results solved by positive matrix factorization (PMF), biomass burning and secondary formation were the major BrC sources in northern Nanjing, with an average total relative contribution of about 90%. Compared to previous studies, diurnal source cycles were added to the PMF simulations in this work by using time‐resolved speciation data, which avoided misclassification of BrC sources.

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Weight loss and abnormal lung inflammation in mice chronically exposed to secondary organic aerosols

Abstract: Chronic exposure to SOAs induces sex-dependent weight loss and abnormal inflammation.

Cited by 3 publications

References 48 publications

Chronic Exposure to Secondary Organic Aerosols Causes Lung Tissue Damage

Chronic Exposure to Secondary Organic Aerosols Causes Lung Tissue Damage

Toxicological Effects of Secondary Air Pollutants

Time‐Resolved Measurements of PM_2.5 Chemical Composition and Brown Carbon Absorption in Nanjing, East China: Diurnal Variations and Organic Tracer‐Based PMF Analysis

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Weight loss and abnormal lung inflammation in mice chronically exposed to secondary organic aerosols

Abstract: Chronic exposure to SOAs induces sex-dependent weight loss and abnormal inflammation.

Cited by 3 publications

References 48 publications

Chronic Exposure to Secondary Organic Aerosols Causes Lung Tissue Damage

Chronic Exposure to Secondary Organic Aerosols Causes Lung Tissue Damage

Toxicological Effects of Secondary Air Pollutants

Time‐Resolved Measurements of PM2.5 Chemical Composition and Brown Carbon Absorption in Nanjing, East China: Diurnal Variations and Organic Tracer‐Based PMF Analysis

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Product

Resources

About

Time‐Resolved Measurements of PM_2.5 Chemical Composition and Brown Carbon Absorption in Nanjing, East China: Diurnal Variations and Organic Tracer‐Based PMF Analysis