Well Differentiated Clear Cell Renal Carcinoma: Significance of Nuclear Deoxyribonucleic Acid Patterns Studied by Flow Cytometry

Abstract

Nuclear deoxyribonucleic acid ploidy was determined in 206 samples of well differentiated clear cell renal carcinoma via a previously described technique on paraffin-embedded archival material. Grade 2 tumors had a higher incidence of abnormal deoxyribonucleic acid patterns than grade 1 tumors. Of stage 1 well differentiated clear cell renal tumors 60 per cent had a normal deoxyribonucleic acid histogram pattern, whereas 67 per cent of higher stage tumors had an abnormal pattern. The existence of abnormal nuclear deoxyribonucleic acid histogram patterns in the primary tumor tissue had a significant correlation with subsequent development of metastatic disease, independent of tumor grade and stage. The 10-year actuarial survival rate was 62 per cent for patients with normal deoxyribonucleic acid histograms and 37 per cent for patients with abnormal patterns. These results demonstrate that nuclear deoxyribonucleic acid ploidy measured by flow cytometry is an important variable in the classification and determination of prognosis for patients with clear cell renal carcinoma.

Expression of Proliferating Cell Nuclear Antigen and Deoxyribonucleic Acid Value in Renal Cell Carcinoma: Correlation with Different Histopathological Parameters and Patient Survival

“…In the present study 63% of the histopathological stage-I tumors were diploid, consistent with the rates found in other reports [34,37,39], although the coefficient of vari ation values are relatively higher. The higher coefficient of variation values might have been caused by fixation artifacts in some of the paraffin-embedded specimens.…”

“…Recently flow cytometry has been used to enhance the understanding of the biological and clinical behavior of genitourinary malignancies [30,31]. A consistent correla tion between ploidy status and the biological behavior of urinary bladder and prostate carcinomas has been noted by different investigators [15,32,33], whereas far less consistent and often contradictory results have been noted in RCC [2,[34][35][36][37][38][39][40]. These discrepancies may relate to intratumor heterogeneity because different assess ments of the ploidy pattern can be made by examining different sites of the same tumor.…”

Appraisal of intratumoral microvessel density, MIB-1 score, DNA content, and p53 protein expression as prognostic indicators in patients with locally confined renal cell carcinoma

“…37 Similarly, in more recent studies of localized RCC, DNA ploidy did not measuring°7 cm in greatest dimension correspond to a classification of pT1 and tumors measuring ú 7 predict survival. 18,19,33 One potential criticism of the DNA ploidy recm but that are still locally confined correspond to a classification of pT2.…”

Prognostic relevance of DNA content in childhood renal tumours

“…All these DNA studies of tumours have shown a great deal of heterogeneity which was unrecognised by conventional histological examination. The association of DNA content with tumour prognosis and progression has given rather conflicting results (Atkin, 1972;Barlogie et al, 1982;Auer et al, 1984;Cornelisse et al, 1984;Moran et al, 1984;Hedley et al, 1984;Douglas et al, 1985;Kreicbergs et al, 1986;Schmidt et al, 1986;Rainwater et al, 1987). Some authors have found a correlation of normal diploid DNA content with good prognosis and aneuploidy with poor prognosis.…”