Well diffusion for antifungal susceptibility testing

Magaldi, Sylvia; Mata-Essayag, Sofía; Capriles, Claudia Hartung de; Pérez, Celina; Colella, María Teresa; Olaizola, Carolina; Ontiveros, Yudith

doi:10.1016/j.ijid.2003.03.002

Cited by 658 publications

(388 citation statements)

References 13 publications

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“…Dimethyl formamide (DMF) was used as a negative control. The experiments were conducted in triplicates (Magaldi, 2004)./…”

Section: Agar-well Diffusion Methodsmentioning

confidence: 99%

Cytotoxic effect of Annona muricata Linn leaves extract on Capan-1 cells

Rosdi¹,

Daud²,

Zulkifli³

et al. 2015

J App Pharm Sci

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A series 305 extracts from 61 traditional medicinal plants (belonging to 33 different families) used in treating skin diseases in Hyderabad Karnataka region were subjected to antidermatophytic screening against Trichophyton rubrum (MTCC 1344). Pet ether, chloroform, ethyl acetate, methanol and aqueous extracts of each plant were tested for their antifungal activity using agar well diffusion method at sample concentration of 5 & 2.5 mg/ml. The results indicated that out of 61 plants, 18 exhibited very effective antidermatophytic activity in methanolic extracts, effective activity observed in 13 plants in different extracts, whereas 26 plants showed moderate activity, 04 plants showed weak activity. The minimum inhibitory concentrations of 18 very effective plants were determined. On the basis of the results obtained, the crude extracts of Allium sativam Linn., Annona reticulata L., Annona squamosa L., Argemone mexicana L., Butea monosperma, Ceasalpinia bonducella, Citrus medica L., Corchorus oleterius L., Emblica officinalis, Euphorbia tirucalli L., Ficus racemosa L., Gymnosporia montana, Lawsonia inermis L., Solanum nigrum L., Sterculia foetida L., Tribulus terrestris L., Vitex negundo L., and Zingiber officinale exhibited significant antidermatophytic activity (T. rubrum) and properties that support folkloric use in the treatment of skin diseases as broad-spectrum antimycotic agents. This probably explains the use of these plants by the indigenous people against dermatological infections.

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“…Dimethyl formamide (DMF) was used as a negative control. The experiments were conducted in triplicates (Magaldi, 2004)./…”

Section: Agar-well Diffusion Methodsmentioning

confidence: 99%

Cytotoxic effect of Annona muricata Linn leaves extract on Capan-1 cells

Rosdi¹,

Daud²,

Zulkifli³

et al. 2015

J App Pharm Sci

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“…These plates were incubated at 37°C for 24 h. The tetracyclin was used as a positive control for bacterial species as commercial control for the antibacterial assay. The antibacterial agents were used in their commercial presentation to prepare a stock solution adjusted to the concentration of 1.25 mg/mL -1 (30 lg per well) (Magaldi et al 2004). All experiments were done in triplicates to obtain concurrent results and the standard deviations were tabulated ( Table 1).…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Synthesis, characterization and magnetic properties of hematite (α-Fe2O3) nanoparticles on polysaccharide templates and their antibacterial activity

Rafi

Ahmed²,

Nazeer

et al. 2014

Appl Nanosci

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The present study is to synthesize iron oxide nanoparticles on different polysaccharide templates calcined at controlled temperature, characterizing them for spectroscopic and magnetic studies leading to evaluate their antibacterial property. The synthesized iron oxide nanoparticles were characterized by X-ray diffractometer (XRD), Fourier transform infrared spectroscopy, high resolution scanning electron microscopy (HRSEM), high resolution transmission electron microscopy (HRTEM) and vibrating sample magnetometer. The iron oxide nanoparticles were tested for antibacterial activity against grampositive and gram-negative bacterial species. The XRD confirms the crystalline nature of iron oxide nanoparticles with the mean crystallite size of 10 nm. The functional groups of the synthesized iron oxide nanoparticles were 547, 543 and 544 cm -1 characterizing the Fe-O and the broad bands at 3,398, 3,439 and 3,427 cm -1 were attributed to the stretching vibrations of hydroxyl group absorbed by iron oxide nanoparticles. HRTEM analyses revealed that the average particle size of the hematite nanoparticles are about 85, 92 and 77 nm for AF, DF and GF, respectively, which was a coincident with the results obtained from the HRSEM analysis. Magnetic measurement exhibited ferromagnetic behavior of the a-Fe 2 O 3 at the room temperature with higher coercivity of H C = 2,303, 2,333 and 1,019 Oe for AF, DF and GF, respectively. Antibacterial test showed the inhibition against Aeromonas hydrophila and Escherichia coli with significant antagonistic activity.

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“…The technique was performed using agar well diffusion (Magaldi et al, 2004) the suspension bacterial cultivation for 24 h from each isolates of E. coli and S. aureus adjusted to 0.5 McFarland turbidity standard described by Padmavathy and Vijayaraghavan (2008). About 100 µL of each bacterial suspension was spreading by swabs onto surface of Mueller-Hinton agar plates and were left (10-15 min) until the surface of agar has dried.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Biosynthesis of Zinc Oxide Nanoparticles and Assay of Antibacterial Activity

Ibrahem¹,

Thalij

Saleh

et al. 2017

American Journal of Biochemistry and Biotechnology

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In this study Out of 280 urinary tract infections samples, 212(75.7%) bacterial isolates were recovered. Based on, morphological, cultural and biochemical testes, further, confirmed the results by VITEK 2 System there were 54 (30.2%) of gram positive whereas the 158 (69.8%) of gram negative. The bacterial isolates were distributed as Escherichia coli followed by Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa and Staphylococcus epidermidis which represented 96(45.2%), 48 (22.6%), 43(20.3%), 14 (6.6%) and 11 (5.2%) respectively. The synthesis of Zinc Oxide nanoparticles (ZnO NPs) using biological methods as eco-friendly from the use of Aspergillus niger filtrate in the extracellular synthesis, then identified and characterized by UV-Vis Spectrophotometer and Scanning Electron Microscopy (SEM). Furthermore, determine the antibacterial efficacy of biological synthesized ZnO NPs against isolated pathogens microbes Staphylococcus aureus and Escherichia coli. The results found the used of 100 mL of ZnCL2 in cultivation of Aspergillus niger was obtained and biosynthesis of ZnO NPs at 46 mg. SEM was illustrate the morphology and practices of ZnO NPs sizes and appear as spherical in shape besides, the size range at 41-75 nm. The assurance by the UV-Vis spectrum appears the absorption bands of ZnO NPs at 380 nm. The results of 1.0 and 1.5 mg mL −1 from ZnO NPs synthesized from A. niger were effect of inhibition against S. aureus and E. coli bacterial isolated from urine tracts infection sources. The Inhibition Zone Diameters (IZD) against S. aureus was appear at 24 and 26 mm respectively compared with the IZD from Ciprofloxacin alone at 20 mm. while the same concentration from ZnO NPs against E. coli causing in IZD at 25 and 28 mm respectively compared with Ciprofloxacin inhibition alone at 23 mm.

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Well diffusion for antifungal susceptibility testing

Cited by 658 publications

References 13 publications

Cytotoxic effect of Annona muricata Linn leaves extract on Capan-1 cells

Cytotoxic effect of Annona muricata Linn leaves extract on Capan-1 cells

Synthesis, characterization and magnetic properties of hematite (α-Fe2O3) nanoparticles on polysaccharide templates and their antibacterial activity

Biosynthesis of Zinc Oxide Nanoparticles and Assay of Antibacterial Activity

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