Cleaning up biocontamination from surfaces is a critical aspect of maintaining a hygienic environment. Traditional cleaning methods often fall short when it comes to eliminating persistent biofilms and resilient bacteria. In recent years, alternative approaches utilizing antibiofilm enzymes, bacteriophages, essential oils (EOs), antibacterial peptides, and biosurfactants have emerged as promising strategies to combat biocontamination. This article explores the efficiency of these agents in targeting and eradicating biofilms, highlighting their mechanisms of action and potential applications. By leveraging the unique properties of these materials, we can enhance cleaning practices and improve public health outcomes by effectively eliminating biocontamination from various surfaces.