What do we measure by a luminol-dependent chemiluminescence of phagocytes?

Vilı́m, Vladimı́r; Wilhelm, J.

doi:10.1016/0891-5849(89)90070-1

Cited by 91 publications

(63 citation statements)

References 50 publications

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“…Reactive oxygen species were monitored byvchemiluminescence (Aitken & Clarkson, 1987a;Aitken et ai, 1990), using 5-amino-2,3-dehydro-l,4 phthalazinedione (luminol) (Vilim & Wilhelm, 1989;Aitken & West, 1990 …”

Section: Reactive Oxygen Speciesmentioning

confidence: 99%

Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors

Aitken¹,

Buckingham²,

Km³

et al. 1992

Reproduction

251

114

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Summary. Cells isolated from the ejaculates of a high proportion of patients exhibiting oligozoospermia are characterized by generation rates of reactive oxygen species that considerably exceed those obtained for the normal fertile population. The purpose of this study was to resolve the cellular source of this enhanced activity. Semen samples from a cohort of oligozoospermic patients and a group of fertile controls were fractionated on discontinuous Percoll gradients to generate three cell populations (0, 50 and 100%) of differing density. For each fraction, both the steady-state and the phorbolester-induced chemiluminescent signals were significantly (P < 0\m=.\001)greater for the oligozoospermic samples than for the fertile controls. In the fertile donors, leucocytes comprised the major source of reactive oxygen species, particularly in the low-density Percoll fractions; in oligozoospermic patients, however, spermatozoa were identified as a second major source of reactive oxygen species. Particularly striking was an intense phorbol-ester-induced chemiluminescent signal generated by oligozoospermic spermatozoa, purified by passage through isotonic Percoll and free of leucocyte contamination, which was 167 times greater than the median signal generated by the corresponding fraction from the fertile controls (P < 0\m=.\001). These results emphasize the importance of spermatozoa as a major source of reactive oxygen species in oligozoospermia and have implications for the diagnosis and treatment of this condition, as well as for the design of appropriate diagnostic strategies.

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Section: Reactive Oxygen Speciesmentioning

confidence: 99%

Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors

Aitken¹,

Buckingham²,

Km³

et al. 1992

Reproduction

251

114

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“…The removal of hypochlorous acid by thiols could therefore sustain the production of luminol chemiluminescence, as observed in this work, by preserving the activity of myeloperoxidase. It is possible, however, that captopril and MPG produced the above effects through a non-specific interaction with the luminol alone as this effect has been previously reported for other reagents [18].…”

Section: Discussionmentioning

confidence: 66%

Effect of captopril, enalaprilat and mercaptopropionyl glycine (MPG) on the oxidative activity of human isolated neutrophils.

Clapperton

Abdullah

et al. 1995

Brit J Clinical Pharma

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1. Neutrophil NADPH oxidase produces the superoxide anion (O2‐) anion radical from oxygen. The thiol containing ACE inhibitor, captopril has been reported to inhibit isolated NADPH oxidase. The above effect of captopril, if present in intact cells, could contribute to the ability of this drug to alleviate neutrophil‐mediated tissue damage. We have, therefore, investigated the effect of captopril on the oxidative activity of intact human isolated neutrophils. 2. The effects of captopril on neutrophil oxidative activity were compared with those of enalaprilat (a non‐thiol ACE inhibitor) and N‐mercaptopropionyl glycine (MPG) (a simple thiol). 3. The oxidative response of PMA‐stimulated neutrophils measured by lucigenin chemiluminescence was not affected by any of these test agents. The thiol captopril and MPG (but not enalaprilat) caused an initial delay in luminol chemiluminescence production by PMA‐stimulated neutrophils. 4. Captopril and MPG (but not enalaprilat) increased, rather than decreased oxygen uptake, when added to PMA‐stimulated neutrophils. Thiol oxidation was determined to be, at least partly, responsible for the excess oxygen uptake observed. 5. NADPH oxidase activity in intact neutrophils was not affected by captopril, MPG or enalaprilat. The inhibition of NADPH oxidase activity is unlikely to contribute to the therapeutic effects of captopril and other thiols.

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“…We measured reactive oxygen species (ROS) by luminol-dependent chemilumines- (Vilim and Wilhelm, 1989) using an assay kit from Calbiochem. Cell pellets were resuspended in superoxide anion medium containing 125 M chemiluminescence enhancer and 100 M luminol.…”

Section: Methodsmentioning

confidence: 99%

Essential Role of C-Rel in Nitric-Oxide Synthase-2 Transcriptional Activation: Time-Dependent Control by Salicylate

Cieslik

Deng²,

2006

Mol Pharmacol

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To determine the role of C-Rel in nitric-oxide synthase-2 (NOS-2) transcriptional activation, we evaluated the effect of lipopolysaccharide and interferon-␥ (LPS/IFN␥) on C-Rel DNA binding in RAW 264.7. LPS/IFN␥-stimulated C-Rel binding peaked at 4 to 8 h and declined at 24 h. Transfection of cells with a C-Rel small interfering RNA abrogated C-Rel binding at all time points. LPS/IFN␥ produced superoxide at 4 h, which subsided at 8 h. C-Rel binding and NOS-2 expression were abrogated by superoxide dismutase or apocynin at 4 h, suggesting a key role that superoxide plays in mediating C-Rel binding and NOS-2 transactivation only at 4 h. We have reported previously that salicylate at 10 Ϫ5

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What do we measure by a luminol-dependent chemiluminescence of phagocytes?

Cited by 91 publications

References 50 publications

Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors

Differential contribution of leucocytes and spermatozoa to the generation of reactive oxygen species in the ejaculates of oligozoospermic patients and fertile donors

Effect of captopril, enalaprilat and mercaptopropionyl glycine (MPG) on the oxidative activity of human isolated neutrophils.

Essential Role of C-Rel in Nitric-Oxide Synthase-2 Transcriptional Activation: Time-Dependent Control by Salicylate

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