What inhalant allergens can do and not do?—The cooperation of allergens and their source in Th2 polarization and allergic sensitization

Grosse-Kathoefer, Sophie; Aglas, Lorenz; Ferreira, Fatima; Pointner, Lisa

doi:10.1007/s40629-023-00262-9

Cited by 4 publications

(1 citation statement)

References 78 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This includes the former Amb a 2 allergen, which was reclassified as Amb a 1.05 based on the high sequence similarity with other Amb a 1 isoforms [28,29]. The immunological characterization of these five isoforms showed varying capacities for IgE binding [30], indicating that isoform Amb a 1.01 exhibits the highest allergenic activity and Amb a 1.01 and Amb a 1.03 are the most potent T cell stimulators [21,31,32].…”

Section: Introductionmentioning

confidence: 99%

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Buzan,

Grijincu,

Zbîrcea

et al. 2024

IJMS

View full text Add to dashboard Cite

Common ragweed pollen allergy has become a health burden worldwide. One of the major allergens in ragweed allergy is Amb a 1, which is responsible for over 90% of the IgE response in ragweed-allergic patients. The major allergen isoform Amb a 1.01 is the most allergenic isoform in ragweed pollen. So far, no recombinant Amb a 1.01 with similar allergenic properties to its natural counterpart (nAmb a 1.01) has been produced. Hence, this study aimed to produce a recombinant Amb a 1.01 with similar properties to the natural isoform for improved ragweed allergy management. Amb a 1.01 was expressed in insect cells using a codon-optimized DNA construct with a removable N-terminal His-Tag (rAmb a 1.01). The recombinant protein was purified by affinity chromatography and physicochemically characterized. The rAmb a 1.01 was compared to nAmb a 1.01 in terms of the IgE binding (enzyme-linked immunosorbent assay (ELISA), immunoblot) and allergenic activity (mediator release assay) in well-characterized ragweed-allergic patients. The rAmb a 1.01 exhibited similar IgE reactivity to nAmb a 1.01 in different IgE-binding assays (i.e., IgE immunoblot, ELISA, quantitative ImmunoCAP inhibition measurements). Furthermore, the rAmb a 1.01 showed comparable dose-dependent allergenic activity to nAmb a 1.01 regarding basophil activation. Overall, the results showed the successful expression of an rAmb a 1.01 with comparable characteristics to the corresponding natural isoform. Our findings provide the basis for an improvement in ragweed allergy research, diagnosis, and immunotherapy.

show abstract

Section: Introductionmentioning

confidence: 99%

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Buzan,

Grijincu,

Zbîrcea

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

Aspergillus-assoziierte Erkrankungen aus infektiologischer und allergologischer Perspektive

Stemler,

Nacov,

Sprute

et al. 2024

Allergo J

View full text Add to dashboard Cite

Aspergillus-associated diseases from an infectious diseases and allergological perspective

Stemler,

Nacov,

Sprute

et al. 2024

Allergo J Int

View full text Add to dashboard Cite

Aspergillus-associated diseases are rare and pose challenges for practitioners. Diagnosis is complex and requires rational, targeted, and multidisciplinary collaboration, as well as a high degree of expertise and an individualized approach. For the infectious diseases physician, the focus is on the question of infection or colonization. In severely immunocompromised patients, invasive aspergillosis occurs, which most frequently affects the lungs (IPA) and is characterized by invasive, destructive growth. This acute clinical picture is associated with a high mortality rate. Chronic pulmonary aspergillosis (CPA) develops on the basis of pre-existing changes in lung structure caused by other pulmonary diseases and often requires surgical treatment. Another chronic form is allergic bronchopulmonary aspergillosis (ABPA). It is often associated with bronchiectasis in patients with bronchial asthma or cystic fibrosis. Sinus mycoses are divided into non-invasive and invasive forms, which can occur in immunocompromised patients and most commonly affect the maxillary sinus. Here, local surgical measures are an obligatory part of treatment, whereas the non-invasive form usually has an allergic component. In addition, drug-based antifungal and/or anti-inflammatory therapy is used for all entities.

show abstract

What inhalant allergens can do and not do?—The cooperation of allergens and their source in Th2 polarization and allergic sensitization

Cited by 4 publications

References 78 publications

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Insect Cell-Expressed Major Ragweed Allergen Amb a 1.01 Exhibits Similar Allergenic Properties to Its Natural Counterpart from Common Ragweed Pollen

Aspergillus-assoziierte Erkrankungen aus infektiologischer und allergologischer Perspektive

Aspergillus-associated diseases from an infectious diseases and allergological perspective

Contact Info

Product

Resources

About