What's next in 'omics: The metabolome

Tachibana, Chris

doi:10.1126/science.345.6203.1519

Cited by 26 publications

(23 citation statements)

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“…Additional information, such as fragmentation data, is essential for structure elucidation of a mass measurement. Putative identifications require matching an experimental MS/MS spectrum with a reference fragmentation spectrum [24, 26]. Metabolomics spectral libraries have been created with experimental data from commercially available or synthesized standards.…”

Section: Lc-ms Based Metabolomics: Strengths and Challengesmentioning

confidence: 99%

Untargeted Metabolomics Strategies—Challenges and Emerging Directions

Schrimpe‐Rutledge

Codreanu

Sherrod

et al. 2016

J. Am. Soc. Mass Spectrom.

951

599

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Metabolites are building blocks of cellular function. These species are involved in enzyme-catalyzed chemical reactions and are essential for cellular function. Upstream biological disruptions result in a series of metabolomic changes, and as such the metabolome holds a wealth of information that is thought to be most predictive of phenotype. Uncovering this knowledge is a work in progress. The field of metabolomics is still maturing; the community has leveraged proteomics experience when applicable and developed a range of sample preparation and instrument methodology along with myriad data processing and analysis approaches. Research focuses have now shifted toward a fundamental understanding of the biology responsible for metabolomic changes. There are several types of metabolomics experiments including both targeted and untargeted analyses. While untargeted, hypothesis generating, workflows exhibit many valuable attributes, challenges inherent to the approach remain. This Critical Insight comments on these challenges, focusing on the identification process of LC-MS based untargeted metabolomics studies – specifically in mammalian systems. Biological interpretation of metabolomics data hinges on the ability to accurately identify metabolites. The range of confidence associated with identifications that is often overlooked is reviewed, and opportunities for advancing the metabolomics field are described.

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Section: Lc-ms Based Metabolomics: Strengths and Challengesmentioning

confidence: 99%

Untargeted Metabolomics Strategies—Challenges and Emerging Directions

Schrimpe‐Rutledge

Codreanu

Sherrod

et al. 2016

J. Am. Soc. Mass Spectrom.

951

599

View full text Add to dashboard Cite

show abstract

“…It is important to mention that, though GC-MS is considered a powerful tool for the identification of volatiles or the compounds able to volatilize through derivation, the identification based on GC-MS faces many challenges like the identification of many isomers and isobars of the volatile compounds, such as lipids. So, there is a need to couple GC with other sensitive mass analysis techniques such as time-of-flight (TOF), a technique providing very high resolution (Tachibana, 2014). The superior performance of two-dimensional GC coupled to TOF (GC×GC/TOF-MS) has been demonstrated (Yao et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

A study revealing the key aroma compounds of steamed bread made by Chinese traditional sourdough

Zhang

Sadiq

et al. 2016

J. Zhejiang Univ. Sci. B

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Aroma of Chinese steamed bread (CSB) is one of the important parameters that determines the overall quality attributes and consumer acceptance. However, the aroma profile of CSB still remains poorly understood, mainly because of relying on only a single method for aroma extraction in previous studies. Therefore, the objective of this study was to determine the volatile aroma compounds of five different samples of CSB using three different aroma extraction methods, namely solid-phase microextraction (SPME), simultaneous distillation-extraction (SDE), and purge and trap (P&T). All samples showed a unique aroma profile, which could be attributed to their unique microbial consortia. (E)-2-Nonenal and (E,E)-2,4-decadienal were the most prevalent aromatic compounds revealed by SDE, which have not been reported previously, while ethanol and acetic acid proved to be the most dominant compounds by both SPME and P&T. Our approach of combining three different aroma extraction methods provided better insights into the aroma profile of CSB, which had remained largely unknown in previous studies.

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“…Metabolomics measures physiology and as such has the ability to link function and genotype with phenotype (35). Metabolomics offers multiple advantages over previous methods of studying embryo metabolism.…”

Section: Metabolomics: a New Approachmentioning

confidence: 99%