1994
DOI: 10.1104/pp.105.1.69
|View full text |Cite
|
Sign up to set email alerts
|

Wheat DNA Primase (RNA Primer Synthesis in Vitro, Structural Studies by Photochemical Cross-Linking, and Modulation of Primase Activity by DNA Polymerases)

Abstract: DNA primase synthesizes short RNA primers used by DNA polymerases to initiate DNA synthesis. Two proteins of approximately 60 and 50 kD were recognized by specific antibodies raised against yeast primase subunits, suggesting a high degree of analogy between wheat and yeast primase subunits. Cel-filtration chromatography of wheat primase showed two active forms of 60 and 110 to 120 kD. Ultraviolet-induced cross-linking with radioactive oligothymidilate revealed a highly labeled protein of 60 kD. After limited t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

1
9
0

Year Published

1996
1996
2007
2007

Publication Types

Select...
5

Relationship

0
5

Authors

Journals

citations
Cited by 14 publications
(10 citation statements)
references
References 24 publications
1
9
0
Order By: Relevance
“…The sizes of the two subunits of the puri®ed tobacco free DNA primase (53 kDa and 63 kDa) are similar to those of the animal and yeast primases associated with DNA polymerase a (48±50 kDa and 58±60 kDa; Wang 1996) and to those of two polypeptides of 50 kDa and 60 kDa which were enriched in a partially puri®ed preparation of a wheat DNA primase (Laquel et al 1994). The tobacco primase sedimented in glycerol gradients at a rate corresponding to an approximate molecular mass of 120 kDa and co-sedimented with two polypeptides of 53 kDa and 63 kDa, indicating that the primase is a heterodimer with no evidence of dissociation.…”
Section: Discussionmentioning
confidence: 91%
See 2 more Smart Citations
“…The sizes of the two subunits of the puri®ed tobacco free DNA primase (53 kDa and 63 kDa) are similar to those of the animal and yeast primases associated with DNA polymerase a (48±50 kDa and 58±60 kDa; Wang 1996) and to those of two polypeptides of 50 kDa and 60 kDa which were enriched in a partially puri®ed preparation of a wheat DNA primase (Laquel et al 1994). The tobacco primase sedimented in glycerol gradients at a rate corresponding to an approximate molecular mass of 120 kDa and co-sedimented with two polypeptides of 53 kDa and 63 kDa, indicating that the primase is a heterodimer with no evidence of dissociation.…”
Section: Discussionmentioning
confidence: 91%
“…It is noteworthy that these primases also synthesise primers of twice and three times the`unit length' at high primase concentrations and in the absence of polymerase, apparently by dissociation followed by reassociation and extension of primers already laid down. A partially puri®ed DNA primase preparation from wheat embryos synthesised``rather long'' primers (size not speci®ed) at high primase concentrations and shorter primers (up to 15 nucleotides) at lower primase concentrations or in the presence of certain DNA polymerases (Graveline et al 1984;Laquel et al 1994). Clear primer bands of twice or more than twice the`unit length' were not synthesised by the tobacco primase, although a faint smear of higher-molecularmass material could be detected.…”
Section: Discussionmentioning
confidence: 94%
See 1 more Smart Citation
“…We have shown previously that wheat DNA primase activity was confined to two proteins of approximately 60 and 50 kDa [19]. Analysis by western blot and immunodetection, as described in Materials and methods, indicate that a specific 50 kDa band is present in the purified fraction of DNA polymerase A, but absent in the purified fraction of DNA polymerase B (data not shown).…”
Section: Dna Primasementioning
confidence: 96%
“…DNA primase activity was measured as reported previously [19]. 50 #1 of incubation mixture contained 50 mM Tris-HC1 pH 8.0, 5 mM magnesium acetate, 1 mM dithiothreitol, 0.5 A260/ml poly(dT) as template and 10 #M [3H]ATP (500-1500 cpm/pmol) as substrate.…”
Section: Dna Primase Activity Assaymentioning
confidence: 99%