When plasma jet is effective for chronic wound bacteria inactivation, is it also effective for wound healing?

Darmawati, Sri; Rohmani, Afiana; Nurani, Laela Hayu; Prastiyanto, Muhammad Evy; Dewi, Sri Sinto; Salsabila, Nia; Wahyuningtyas, Eka Sakti; Murdiya, Fri; Sikumbang, Isabella Meliawati; Rohmah, Ratnasari Nur; Fatimah, Yun Arifatul; Widiyanto, Andi; Ishijima, Tatsuo; Sugama, Junko; Nakatani, Toshio; Nasruddin,

doi:10.1016/j.cpme.2019.100085

Cited by 23 publications

(31 citation statements)

References 21 publications

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“…These reaction-discharge systems include dielectric barrier discharges (DBDs) [14,15], corona discharges (CDs) [37], microwave-induced plasmas (MIPs) [11,12], and plasma jets named kINPen [5,[7][8][9][10]17] or COST [38,39], in addition to the non-thermal atmospheric pressure plasma jets studied and utilized by e.g. Rad et al [40], Gidon et al [41,42], Darmawati et al [43], Cheng et al [44], Busco et al [45]. To be applied in biological systems, the reaction-discharge systems should provide a low gas temperature, i.e., less than 40 °C, along with the controlled production of reactive chemical compounds such as ROS and RNS, and the emission of UV radiation [9].…”

Section: Introductionmentioning

confidence: 99%

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Dzimitrowicz

Bielawska‐Pohl

Jamróz

et al. 2019

Plasma Chem Plasma Process

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Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. For that reason, controlled promotion of these processes is required. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature T rot (OH), a design of experiment followed by a Response Surface Methodology was applied. Based on the suggested statistical model, the optimal operating conditions under which the T rot (OH) is less than 37 °C (310 K) were estimated. Then, the resulted model was validated in order to confirm its accuracy. After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Additionally, the qualitative and quantitative analyses of the reactive oxygen species and reactive nitrogen species were performed in order to investigate of reactions and processes running in the He-DBD-gaseous phase and in the He-DBD-treated liquid. Next, the developed portable He-DBD-based reaction-discharge system, working under the optimal operating conditions, was used to stimulate the wound healing process. It was found that a 30 s He-DBD treatment significantly increased the proliferation, migration, and angiogenesis of keratinocytes (HaCaT) and fibroblasts (MSU-1.1) cell lines, as well as human skin microvascular endothelial cells (HSkMEC.2). Hence, the application of the cold atmospheric pressure plasma generated in this He-DBD-based reaction-discharge system might be an alternative therapy for patient suffering from chronic wounds. Keywords Wound healing • Angiogenesis • Migration • Proliferation • Cold atmospheric pressure plasma Abbreviations BBD Box-Behnken design Anna Dzimitrowicz and Aleksandra Bielawska-Pohl have equally contributed to this work.

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Section: Introductionmentioning

confidence: 99%

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Dzimitrowicz

Bielawska‐Pohl

Jamróz

et al. 2019

Plasma Chem Plasma Process

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“…The presence of pilli and fimbriae in most of the Gram negative bacteria including S. Typhi plays an important role in attaching the bacteria to the surface of host cell mucous membranes and initiate an infection [22]. The little compromised of AB serogroupings in the development of S. typhi infection may likely be due to the presence of A and B antigen which serve as the complementary adhesion factor of S. typhi as reported by Kundera et al [23] and Busch et al [24].…”

Section: Discussionmentioning

confidence: 90%

Evaluation of in vitro bactericidal activity of human serum against Salmonella Typhi in relation to serogroups

Aliyu

Ismaila

Haruna

et al. 2020

Microbes and Infectious Diseases

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Background: Series of more than 35 proteins facilitated a major role in fighting the foreign invaders in human body and other warm blooded animals, those named complement system. Methods: Total of 147 human serum were collected from asymptomatic volunteers by venipuncture, their sero groups were determined by reverse blood grouping method. Strain of Salmonella typhi (S. Typhi) were collected from Wudil general hospital and identified molecularly at center of biotechnological research of Bayero University, Kano. Serum bactericidal assay was done at microbiology laboratory of Wudil general hospital. Data obtained was subjected to two way ANOVA and considered significant at p<0.05. Results: Complement dependent pathways shows a more than 50% kills, in which both A, AB, B and O kill percent were 74.98%, 67.87%, 78.53% and 78.60% respectively, however, no statistical significant difference. While the non-complement dependent pathways revealed kills percentage of 59.27%, 57.86%, 59.21% and 58.60% for A, AB, B and O sero groups, also no significant statistical difference. Moreover, in comparing the complement and non-complement dependent pathways, data analysis shows a significant difference with p values of 0.001, 0.003, 0.006 and 0.0008 for A, AB, B and O sero groups respectively. Conclusion: We concluded that, ABO sero-groups and complement system plays an important role as infection determinants, where AB sero-group have more chances to S. Typhi infection than other sero-groupings. This study suggests that the effect of many host genetic parameters on treatment of microorganisms needs to be further studied.

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“…The wound healing-promoting effect of an argon plasma jet was enhanced if the number of gas plasma treatment sessions was adapted [ 44 ], as it was shown that long gas plasma exposure times, in general, can lead to wound necrosis [ 45 ]. Leveled gas plasma exposure, by contrast, supports re-epithelization [ 46 ] and changes in collagen and vimentin [ 47 ] and αSMA (alpha-smooth muscle actin) deposition [ 48 ]. Especially for nitrogen (N 2 ) plasma jets, positive effects on wound contraction were observed [ 49 ], which was accompanied by elevated RNS deposition [ 50 ] and laminin production, along with decreased MMP (matrix metalloproteinase)-3 expression [ 51 ].…”

Section: Gas Plasma Treatment Of Skin and Wounds In Animal And Veterinary Modelsmentioning

confidence: 99%

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

2021

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The loss of skin integrity is inevitable in life. Wound healing is a necessary sequence of events to reconstitute the body’s integrity against potentially harmful environmental agents and restore homeostasis. Attempts to improve cutaneous wound healing are therefore as old as humanity itself. Furthermore, nowadays, targeting defective wound healing is of utmost importance in an aging society with underlying diseases such as diabetes and vascular insufficiencies being on the rise. Because chronic wounds’ etiology and specific traits differ, there is widespread polypragmasia in targeting non-healing conditions. Reactive oxygen and nitrogen species (ROS/RNS) are an overarching theme accompanying wound healing and its biological stages. ROS are signaling agents generated by phagocytes to inactivate pathogens. Although ROS/RNS’s central role in the biology of wound healing has long been appreciated, it was only until the recent decade that these agents were explicitly used to target defective wound healing using gas plasma technology. Gas plasma is a physical state of matter and is a partially ionized gas operated at body temperature which generates a plethora of ROS/RNS simultaneously in a spatiotemporally controlled manner. Animal models of wound healing have been vital in driving the development of these wound healing-promoting technologies, and this review summarizes the current knowledge and identifies open ends derived from in vivo wound models under gas plasma therapy. While gas plasma-assisted wound healing in humans has become well established in Europe, veterinary medicine is an emerging field with great potential to improve the lives of suffering animals.

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When plasma jet is effective for chronic wound bacteria inactivation, is it also effective for wound healing?

Cited by 23 publications

References 21 publications

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Activation of the Normal Human Skin Cells by a Portable Dielectric Barrier Discharge-Based Reaction-Discharge System of a Defined Gas Temperature

Evaluation of in vitro bactericidal activity of human serum against Salmonella Typhi in relation to serogroups

Gas Plasma-Augmented Wound Healing in Animal Models and Veterinary Medicine

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