Which came first: The disease or the pest? Is there a host mediated spread of Beauveria bassiana (Ascomycota: Hypocreales) by invasive palm pests?

González-Mas, Natalia; Ortega-García, L.; Garrido‐Jurado, Inmaculada; Dembilio, Óscar; Miret, Josep Anton Jaques; Quesada‐Moraga, Enrique

doi:10.1016/j.jip.2019.01.007

Cited by 11 publications

(4 citation statements)

References 60 publications

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“…The suspensions were then filtered through sterile gauze to remove mycelial debris and, after that, conidia concentrations were evaluated at optical microscope by counting at Bürker chamber. Suspensions were adjusted with sterile distilled water to obtain the final volume of 15 mL (5 mL per replica, for each strain) and the concentration of 1*10 8 conidia/mL, as reported by other authors for controlled condition trials (González-Mas et al 2019, Moussa et al 2021. The suspensions were added with 0.05 v/v Tween 80® (v/v) to break the surface tension of water and evenly distribute the conidia, and vortex-mixed for 2-3 min.…”

Section: Fungal Strains and Preparation Of Conidia Inoculummentioning

confidence: 99%

Activity of natural occurring entomopathogenic fungi on nymphal and adult stage ofPhilaenus spumarius

Bodino

Barbera

González-Mas

et al. 2023

Preprint

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The spittlebug Philaenus spumarius (Hemiptera: Aphrophoridae) is the predominant vector of Xylella fastidiosa (Xanthomonadales: Xanthomonadaceae) in Apulia and Europe. Current control strategies of the insect vector rely on mechanical management of nymphal stages and insecticide application against adult populations. Entomopathogenic fungi (EPF) are biological control agents naturally attacking spittlebugs and may effectively reduce population levels of host species. Different experimental trials in controlled conditions have been performed to i) identify naturally occurring EPF on P. spumarius in Northwestern Italy, and ii) evaluate the potential for biocontrol of the isolated strains on both nymphal and adult stages of the spittlebug. Four EPF species were isolated from dead P. spumarius cadavers collected in semi-field conditions: Beauveria bassiana, Conidiobolus coronatus, Fusarium equiseti and Lecanicillium aphanocladii. All the fungal isolates showed entomopathogenic potential against nymphal stages of P. spumarius (approximately 45 % mortality), except for F. equiseti, in preliminary trials. No induced mortality was observed on the adult stage. Lecanicillium aphanocladii was the most promising fungus and its pathogenicity against spittlebug nymphs was further tested in different formulations (conidia vs blastospores) and with natural adjuvants. Blastospore formulation was the most effective in killing nymphal instars and reducing the emergence rate of P. spumarius adults, reaching mortality levels (90%) similar to those of the commercial product Naturalis®, while no or adverse effect of natural adjuvants was recorded. The encouraging results of this study pave the way for testing EPF isolates against P. spumarius in field conditions and find new environmentally friendly control strategies against insect vectors of X. fastidiosa.

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Section: Fungal Strains and Preparation Of Conidia Inoculummentioning

confidence: 99%

Activity of natural occurring entomopathogenic fungi on nymphal and adult stage ofPhilaenus spumarius

Bodino

Barbera

González-Mas

et al. 2023

Preprint

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“…Thus, feeding activity decreased due to insecticide The difference in mortality rates was due to an increase in the number of B. bassiana conidia contained in the treatment suspension. The higher the concentration of the B. bassiana formulation, the higher the B. bassiana conidia content and the higher the chance of infection (González-Mas et al, 2019). The mortality rate achieved was not optimal because the application of B. bassiana was only on the leaves of the early feed.…”

Section: Antifeedant Effectmentioning

confidence: 99%

In Vitro Effectiveness of Beauveria bassiana as a Control Agent against Invasive Fall Armyworm (Spodoptera frugiperda) Larvae

Purwanti

Mulyasari

Budianto

2022

JPTI

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Fall armyworm or Spodoptera frugiperda is one of the main pests of corn. It can caused up to 28.08% yield loss. Pest control efforts with broad-spectrum insecticides can cause negative impacts that disrupt ecosystems, non-target biotic factors and the surrounding environment. Beauveria bassiana is an alternative biological control agent that is target-specific. This study was conducted to determine toxic and antifeedant activities of B. bassiana application on third to fifth-instar S. frugiperda larvae. B. bassiana isolates were propagated using corn flakes media and resulted in colonies with conidia density of 1.1×108conidia.g-1. This study used a completely randomized design with 5 treatments and 5 replications. Treatments consisted of P1 namely B. bassiana propagation with a concentration of 60 g.L-1, P2 = 70 g.L-1, and P3 = 80 g.L-1. P0 was a negative control (untreated with B. bassiana) and P4 was a positive control (diazinon with concentrations of 2 ml.L-1). The variables observed were mortality and weight of the leftover feed. The results showed that the formulation of B. bassiana with concentrations of 80 g.L-1 was able to infect armyworms with a mortality percentage of 53.5% which was statistically similar as the effect of chemical insecticides. In addition, inhibition of feeding activity due to treatment could prevent leaf damage up to 68.21%.

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“…However, it has been shown that this practice leads to serious environmental pollution and also entails high costs for emergency response (Rehner et al, 2011;Kepler and Rehner, 2013;Kovač et al, 2020). To mitigate the serious environmental problems that have arisen as a result of the continued overuse of chemical pesticides, biopesticides based on entomopathogenic fungi such as Beauveria bassiana (Rehner et al, 2011;Kepler and Rehner, 2013;Kovač et al, 2020) and Beauveria pseudobassiana (González-Mas et al, 2019;Amobonye et al, 2020;Dannon et al, 2020) are increasingly being used to control insect outbreaks. The use of these biopesticides is an effective means of biological control of locust and grasshopper pests, helps to avoid chemical residues in agricultural products, destroys a large number of locusts in cloudy conditions, and changes their morphological phase transformation of insects, preventing the formation of dense wings and swarms of locusts (Wang et al, 2020;Kovač et al, 2021;Altimira et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Effective Strains of Beauveria Bassiana and B. Pseudobassiana used Against the Asian Locust (Locusta Migratoria L.) in Kazakhstan

Zhumataуeva¹,

Duissembekov²,

Dutbayev³

et al. 2022

OnLine Journal of Biological Sciences

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The purpose of the study was to perform genetic identification of the strains of fungi of the Beauveria genus and evaluate the effect of abiotic factors on the growth and survival of colonies and the productivity of conidia of these fungi, as well as to determine the biological effectiveness of the semi-preparative form of the drug based on Beauveria bassiana in the field. Conventional and modern methods accepted in biology and mycology were used. Identification of strains of Beauveria fungi was carried out by determining the direct nucleotide sequence of the TEF region. Laboratory experiments were carried out to evaluate various factors on the diameter of colony growth, survival in deep and surface cultivation, the type of semi-preparative form of the drug based on B. bassiana and the time of its exposure, and the effect on the biological effects of drugs in the field. Statistical data processing was carried out in the R studio program using the P value. The productivity of conidia of entomopathogenic fungi during surface cultivation depended on the type of strain and the duration of cultivation of conidia. The diameter of the growth of Beauveria fungi colonies was influenced by the factors of the strain, the type of nutrient medium, the temperature of colony cultivation, and the number of days after the start of colony cultivation. The survival of conidia of the B. bassiana fungus on the wings of Locusta migratoria L. orrelated with the indicators of cultivation temperature and germination of conidia and cultivation time. The productivity of Beauveria spp. conidia correlate with the type of strain, the type of substrate, and the time of exposure. The conidia continued to germinate on the 25 th day. Fungi grew better on a substrate based on millet. In the field, the mortality rate of L. migratoria L. and the biological effectiveness of biopesticides were influenced by the factors of the strain, the form of spraying with the bio preparation, and the time elapsed after treatment with the biopesticide.

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Which came first: The disease or the pest? Is there a host mediated spread of Beauveria bassiana (Ascomycota: Hypocreales) by invasive palm pests?

Cited by 11 publications

References 60 publications

Activity of natural occurring entomopathogenic fungi on nymphal and adult stage ofPhilaenus spumarius

Activity of natural occurring entomopathogenic fungi on nymphal and adult stage ofPhilaenus spumarius

In Vitro Effectiveness of Beauveria bassiana as a Control Agent against Invasive Fall Armyworm (Spodoptera frugiperda) Larvae

Effective Strains of Beauveria Bassiana and B. Pseudobassiana used Against the Asian Locust (Locusta Migratoria L.) in Kazakhstan

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