Which Coagulation Factors Interfere with the One-Stage Prothrombin Time?

Talstad, Ingebrigt

doi:10.1159/000216848

Cited by 8 publications

(8 citation statements)

References 14 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In our experiment, only the factor X fulfilled this requirement. This result is consistent with the hypothesis of Kerins and MacNicoll [37] that the factor X has greater effect on the prothrombin time of rats than others, whereas, in human, the dilution assays and the mathematical model results give prothrombin as the critical factor for the prothrombin time [36,38]. This relation between prothrombin time and factor X might be useful in order to refine ED50 test.…”

Section: Assessmentsupporting

confidence: 89%

Monitoring of antivitamin K-dependent anticoagulation in rodents – Towards an evolution of the methodology to detect resistance in rodents

Lefebvre

Hascoët

Damin-Pernik

et al. 2017

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

Vitamin K antagonists are used as rodenticides for pest control management. In rodents, prothrombin time is used to monitor their effect despite its limits and the emergence of many coagulation methods. The aim of this study is to explore different coagulation monitoring methods in order to propose the best method and the best parameter to monitor vitamin K antagonists effect in rodents.The coagulation function was thus monitored with global coagulation assays and specialty assays after difethialone administration in rats.Despite many parameters obtained by thromboelastometry, only clotting time and clot formation time obtained by ExTEM were modified. Their evolution was fast with doubling time 1 respectively of 4.0h and 3.7h but their increases were delayed with a lag time higher than 8h.Conversely, prothrombin time evolution presented a lag time of only 2h, but a higher doubling time of 7.2h. The measurements of factor VII and X activities were the most sensitive assays to monitor vitamin K antagonists effect with almost no lag time and the fastest evolution.Nevertheless, factor X was shown to be the only key factor driving prothrombin time.Monitoring factor X activity enables to follow most effectively the anticoagulation status in rats after rodenticides administration.

show abstract

Section: Assessmentsupporting

confidence: 89%

Monitoring of antivitamin K-dependent anticoagulation in rodents – Towards an evolution of the methodology to detect resistance in rodents

Lefebvre

Hascoët

Damin-Pernik

et al. 2017

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

show abstract

“…However, it has been reported that the PT clotting time is not equally influenced by the plasma activity of each clotting factor, rather it is largely influenced by the activity of factors VII and X but not that of other factors. 20 We believe that the specific effects of these vitamin K-dependent factors on the INR value explains why the INR is more closely correlated to the plasma factor X level than the PT activity percent.…”

Section: Discussionmentioning

confidence: 95%

Usefulness and accuracy of the international normalized ratio and activity percent of prothrombin time in patients with liver disease

Takikawa

Mari

Wang

et al. 2013

Hepatology Research

View full text Add to dashboard Cite

show abstract

“…Anticoagulant therapy decreases the liver coagulation factors II, VII, IX, X, protein C and S, of which factor VII may be the dominant factor [15]. The Pivka inhibitor does not have any clinical relevance Talstad and does not change the linearity between coagulation activities.…”

Section: Discussionmentioning

confidence: 99%

Why Is the Standardization of Prothrombin Time a Problem?

Talstad

2000

Pathophysiol Haemos Thromb

Self Cite

View full text Add to dashboard Cite

The standardization of prothrombin time (PT) has been a problem for more than 50 years; by applying the methods to the same clinical material the analysis may explain why. The initial standardization by 2–3 times normal clotting time by Quick’s PT assay did not standardize presently used PT assays. The ratio method accepted by the WHO in 1977 was not satisfactory because the intercept was neglected. The revised ratio method (WHO 1983) recommended calibration between log clotting times of normal and abnormal plasmas and a simplified conversion into ratios. However, by including a high proportion of normal plasmas (up to 1/3 of abnormal) an erroneous calibration material was introduced; the conversion from clotting times into ratios was also more complex than predicted. The international sensitivity index, which is based on the simplified method, produced international normalized ratios (INR) that were not more standardized than not corrected ratios. Not corrected coagulation activities showed poor standardization due to the different sensitivity of assays for the protein induced by vitamin K absence (Pivka) inhibitor. The corresponding therapeutic ranges of INR and coagulation activities covered 70 and 10–20% of their scales, respectively. Standardization by Pivka-corrected coagulation activities against an international reference assay may be the preferable method.

show abstract

Which Coagulation Factors Interfere with the One-Stage Prothrombin Time?

Cited by 8 publications

References 14 publications

Monitoring of antivitamin K-dependent anticoagulation in rodents – Towards an evolution of the methodology to detect resistance in rodents

Monitoring of antivitamin K-dependent anticoagulation in rodents – Towards an evolution of the methodology to detect resistance in rodents

Usefulness and accuracy of the international normalized ratio and activity percent of prothrombin time in patients with liver disease

Why Is the Standardization of Prothrombin Time a Problem?

Contact Info

Product

Resources

About