White bass Morone chrysops is less susceptible than its hybrid to experimental infection with Flavobacterium columnare

Abstract

Hybrid striped bass (HSB) and white bass (WB) were evaluated for their susceptibility to Flavobacterium columnare, the causative agent of columnaris disease, in 3 fundamental studies. In the first experiment, we determined whether columnaris disease could be developed by experimental challenge in HSB. This challenge consisted of 3 levels of F. columnare (10, 30, and 60 ml volumes) determined to be 2.25 × 10 7 , 6.75 × 10 7 , and 1.35 × 10 8 CFU ml −1 , respectively. Each treatment group exhibited significantly different survival rates: 0, 3.3, and 13.3% in the 60, 30, and 10 ml groups, respectively. In Expt 2, using the 30 ml dose, both HSB and WB had a 0% survival rate, with WB taking significantly longer to reach 100% mortality. In Expt 3, using the 10 ml dose, no HSB survived, whereas 33% of WB survived (p < 0.0001). Compared to controls, HSB treated with 10 ml showed extensive gill damage at 24 h, which could have contributed to the higher mortality observed in HSB; in contrast, WB gills showed noticeably less damage. From these series of experiments, it is clear that HSB are more sensitive to F. columnare, having lower survival and more extensive histological damage compared to WB following challenge. KEY WORDS: Columnaris disease · Hybrid striped bass · Dose response · Differential survivalResale or republication not permitted without written consent of the publisher

Differential susceptibility of white bass (Morone chrysops), striped bass (Morone saxatilis) and hybrid striped bass (M. chrysops × M. saxatilis) to Flavobacterium columnare and effects of mucus on bacterial growth and biofilm development

“…Flavobacterium columnare isolate LSU‐066‐04 was used for the current study because it was originally isolated from largemouth bass ( Micropterus salmoides ) at the Louisiana Aquatic Diagnostic Lab and the previous work done in moronids model (Beck et al., 2015; Fuller et al., 2014; Lange et al., 2017). The F. columnare isolate was retrieved from frozen glycerol stocks and streaked onto F. columnare growth medium (FCGM) (Darwish et al., 2008; Farmer, 2004).…”

“…Disease susceptibility has been shown to vary depending on fish species and pathogen (Arias et al., 2012; Griffin et al., 2010; Reichley et al., 2018; Xu et al., 2014). However, previous research showed that WB exhibit superior survival to disease challenges with F. columnare , as compared to hybrid striped bass (Fuller et al., 2014).…”

A comparison of high‐ and low‐virulence Flavobacterium columnare strains reveals differences in iron acquisition components and responses to iron restriction

“…Recent studies found that the LSU isolate was highly virulent in white bass Morone chrysops , hybrid striped bass Morone chrysops x Morone saxatilis and channel catfish (Beck et al . ; Fuller, Farmer & Beck ). Building upon these previous reports, the present study is the first head‐to‐head comparison of these two isolates in a single host species.…”

SNP panel development for genetic management of wild and domesticated white bass (Morone chrysops)

“…Genetic and genomic tools are needed to support the ongoing domestication and selective breeding efforts of the USDA‐ARS Stuttgart National Aquaculture Research Center (SNARC; Stuttgart, AR), aimed at providing the industry with a superior and sustainable, cultured broodstock source (Fuller & McEntire ; Fuller et al . , ; Li et al . ).…”