White spot syndrome virus quantification in blue crab Portunus trituberculatus hatchery-produced larvae and wild populations by TaqMan real-time PCR, with an emphasis on the relationship between viral infection and crab health

Meng, Xianhong; Jang, In‐Kwon; Seo, Hyung-Chul; Cho, Yeong-Roc

doi:10.1016/j.aquaculture.2009.02.003

Cited by 39 publications

(15 citation statements)

References 26 publications

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“…Most of the research on this topic to date has involved white spot syndrome virus (WSSV) in crustaceans. The average viral load for LaCopCV is on the same order of magnitude as estimated WSSV loads in postlarvae of Fenneropenaeus chinensis (34), and approximately one order of magnitude higher than WSSV loads in Portunuus trituberculatus larvae obtained from diverse locations in Korea (35). The range of LaCopCV viral loads observed is similar to ranges reported in surveys of WSSV among P. trituberculatus larvae (35).…”

Section: Atcopcv Lacopcvsupporting

confidence: 69%

“…This finding indicates a high prevalence of the virus among wild L. aestiva populations and is consistent with reports of high incidences of viral infections in other marine arthropods, including penaeid shrimp (34)(35)(36)(37). Currently, quantitative data on DNA viruses in marine arthropods are sparse.…”

Section: Atcopcv Lacopcvsupporting

confidence: 69%

“…However, previous studies have focused on virus pathology, and few estimates of viral load or prevalence for marine crustacea have been published. To examine the viral load of LaCopCV within individual animals, we applied a quantitative PCR (qPCR) approach used previously to study viral loads in larvae of aquacultured prawns (34,35). qPCR of the putative capsid gene of LaCopCV revealed viral loads ranging from 77 to 7.75 × 10 5 copies per L. aestiva individual, with an average viral load of 1.13 × 10 5 copies per individual copepod and 100% of L. aestiva individuals infected in three of the four Fig.…”

Section: Resultsmentioning

confidence: 99%

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Molecular and microscopic evidence of viruses in marine copepods

Dunlap

Rosario

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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As dominant members of marine mesozooplankton communities, copepods play critical roles in oceanic food webs and biogeochemical cycling. Despite the ecological significance of copepods, little is known regarding the causes of copepod mortality, and up to 35% of total copepod mortality cannot be accounted for by predation alone. Viruses have been established as ecologically important infectious agents in the oceans; however, viral infection has not been investigated in mesozooplankton communities. Here we used molecular and microscopic techniques to document viral infection in natural populations of the calanoid copepods Acartia tonsa (Dana) and Labidocera aestiva (Wheeler) in Tampa Bay, FL. Viral metagenomics revealed previously undocumented viruses in each species, named Acartia tonsa copepod circo-like virus (AtCopCV) and Labidocera aestiva copepod circo-like virus (LaCopCV). LaCopCV was found to be extremely prevalent and abundant in L. aestiva populations, with up to 100% prevalence in some samples and average viral loads of 1.13 × 10 5 copies per individual. LaCopCV transcription was also detected in the majority of L. aestiva individuals, indicating viral activity. AtCopCV was sporadically detected in A. tonsa populations year-round, suggesting temporal variability in viral infection dynamics. Finally, virus-like particles of unknown identity were observed in the connective tissues of A. tonsa and L. aestiva by transmission electron microscopy, demonstrating that viruses were actively proliferating in copepod connective tissue as opposed to infecting gut contents, parasites, or symbionts. Taken together, these results provide strong independent lines of evidence for active viral infection in dominant copepod species, indicating that viruses may significantly influence mesozooplankton ecology.

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Section: Atcopcv Lacopcvsupporting

confidence: 69%

Section: Atcopcv Lacopcvsupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

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Molecular and microscopic evidence of viruses in marine copepods

Dunlap

Rosario

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…A descarga de água dos cultivos de camarões tem sido apontada como responsável pela contaminação da população natural de decápodes e outros organismos suscetíveis (Lo et al, 1996a,b) e pela alta prevalência de WSSV em organismos silvestres (Meng et al, 2009).…”

Section: -216 2012unclassified

Presença do vírus da síndrome da mancha branca em crustáceos decápodes silvestres em lagoas costeiras no Sul do Brasil

Costa¹,

Fraga

Zamparetti

et al. 2012

Arq. Bras. Med. Vet. Zootec.

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“…Infectious hypodermal and haematopoietic necrosis virus (IHHNV) and hepatopancreatic parvovirus (HPV) also cause economic losses to the farmers, although the mortality by these diseases is less than that of WSSV (Fauce et al, 2007;Xia et al, 2015). Different species of crabs very often act as carriers of WSSV transmission to cultured shrimps (Meng et al, 2009). The wild crab Uca spp.…”

mentioning

confidence: 99%

Occurrence of pathogenic shrimp viruses in selected wild crab species of Sunderban, India

2016

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Diseases in brackishwater aquaculture systems are the major hindrance to the growth of shrimp industry in India, with white spot viral disease being the most devastating one causing crop failure leading to heavy economic losses. In this study, wild crab species viz., Uca spp. and Sesarma spp., which are frequent dwellers surrounding the shrimp culture ponds, were screened for the presence of white spot syndrome virus (WSSV), infectious hypodermal and haematopoietic necrosis virus (IHHNV) and hepatopancreatic parvovirus (HPV). Out of total 66 samples screened, presence of WSSV and IHHNV was detected in 18.2 and 1.5% of the samples, respectively. Results of the present study indicate that wild crab species found in and around shrimp ponds could act as carriers of shrimp pathogenic viruses and their effective control is essential for prevention of shrimp viral diseases.

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White spot syndrome virus quantification in blue crab Portunus trituberculatus hatchery-produced larvae and wild populations by TaqMan real-time PCR, with an emphasis on the relationship between viral infection and crab health

Cited by 39 publications

References 26 publications

Molecular and microscopic evidence of viruses in marine copepods

Molecular and microscopic evidence of viruses in marine copepods

Presença do vírus da síndrome da mancha branca em crustáceos decápodes silvestres em lagoas costeiras no Sul do Brasil

Occurrence of pathogenic shrimp viruses in selected wild crab species of Sunderban, India

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