Whole blood transcriptome analysis in feedlot cattle after 35 days of supplementation with a β1-adrenergic agonist

Burrack, Rachel M; Duffy, Erin M; Yates, Dustin T; Schmidt, Ty B; Petersen, Jessica L

doi:10.1007/s13353-019-00527-6

Cited by 4 publications

(1 citation statement)

References 20 publications

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“…As a beta-adrenergic stimulator, RHC was thought to be associated with the stress response, resulting in metabolic inflammatory pathways activation. Actually, the blood transcriptomic analysis after 35 days of supplementation evidenced the IFP35, as well as TYROBP and TP53INP1 as DEPs without a systemic dysregulation of inflammatory pathways, given no induction of TNF-alpha, IL-6, and IL-10 [ 92 ]. Therefore, the RHC-induced upregulation of IFP35 is associated with the cattle weight increase, but not to the systemic inflammation.…”

Section: Ifp35 In the Pathologymentioning

confidence: 99%

IFP35 Is a Relevant Factor in Innate Immunity, Multiple Sclerosis, and Other Chronic Inflammatory Diseases: A Review

Masi

Orlando

Bagordo

et al. 2021

Biology

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Discovered in 1993 by Bange et al., the 35-kDa interferon-induced protein (IFP35) is a highly conserved cytosolic interferon-induced leucine zipper protein with a 17q12-21 coding gene and unknown function. Belonging to interferon stimulated genes (ISG), the IFP35 reflects the type I interferon (IFN) activity induced through the JAK-STAT phosphorylation, and it can homodimerize with N-myc-interactor (NMI) and basic leucine zipper transcription factor (BATF), resulting in nuclear translocation and a functional expression. Casein kinase 2-interacting protein-1 (CKIP-1), retinoic acid-inducible gene I (RIG-I), and laboratory of genetics and physiology 2 Epinephelus coioides (EcLGP2) are thought to regulate IFP35, via the innate immunity pathway. Several in vitro and in vivo studies on fish and mammals have confirmed the IFP35 as an ISG factor with antiviral and antiproliferative functions. However, in a mice model of sepsis, IFP35 was found working as a damage associated molecular pattern (DAMP) molecule, which enhances inflammation by acting in the innate immune-mediated way. In human pathology, the IFP35 expression level predicts disease outcome and response to therapy in Multiple Sclerosis (MS), reflecting IFN activity. Specifically, IFP35 was upregulated in Lupus Nephritis (LN), Rheumatoid Arthritis (RA), and untreated MS. However, it normalized in the MS patients undergoing therapy. The considered data indicate IFP35 as a pleiotropic factor, suggesting it as biologically relevant in the innate immunity, general pathology, and human demyelinating diseases of the central nervous system.

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Section: Ifp35 In the Pathologymentioning

confidence: 99%

IFP35 Is a Relevant Factor in Innate Immunity, Multiple Sclerosis, and Other Chronic Inflammatory Diseases: A Review

Masi

Orlando

Bagordo

et al. 2021

Biology

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MicroRNA-155-5p regulates the Th1/Th2 cytokines expression and the apoptosis of group 2 innate lymphoid cells via targeting TP53INP1 in allergic rhinitis

Zhu

Yuan

et al. 2021

International Immunopharmacology

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Transcriptome analyses indicate that heat stress-induced inflammation in white adipose tissue and oxidative stress in skeletal muscle is partially moderated by zilpaterol supplementation in beef cattle

Reith

Sieck

Grijalva

et al. 2022

Journal of Animal Science

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Heat stress (HS) triggers oxidative stress, systemic inflammation, and disrupts growth efficiency of livestock. β-adrenergic agonists supplemented to ruminant livestock improve growth performance, increase skeletal muscle mass and decrease carcass fat. The objective of this study was to understand the independent and interacting effects of HS and zilpaterol hydrochloride (ZH) supplementation on the transcriptome of subcutaneous white adipose tissue and the longissimus dorsi muscle in steers. Twenty-four Red Angus-based steers were assigned to thermoneutral (TN; Temperature Humidity Index (THI)=68) or HS (THI=73-85) conditions and were not supplemented or supplemented with ZH (8.33 mg/kg/day) for 21 d in a 2x2 factorial. Steers in the TN condition were pair-fed to the average daily feed intake of HS steers. RNA was isolated from adipose tissue and skeletal muscle samples collected via biopsy on 3, 10, and 21 d and sequenced using 3’ Tag-Seq to an achieved average depth of 3.6 million reads/sample. Transcripts, mapped to ARS-UCD1.2, were quantified. Differential expression (DE) analyses were performed in DESeq2 with a significance threshold for false discovery rate of 0.05. In adipose, 4 loci (MISP3, APOL6, SLC25A4, S100A12) were DE due to ZH on d 3, and 2 (RRAD, ALB) were DE due to the interaction of HS and ZH on d 10 (Padj<0.05). In muscle, 40 loci (including TENM4 and OAZ1) were DE due to ZH on d 10 and 6 loci (HIF1A, LOC101903734, PDZD9, HNRNPU, MTUS1, TMCO6) were DE due to environment on d 21 (Padj<0.05). To explore biological pathways altered by environment, supplement, and their interaction, loci with DE (Praw<0.05) were evaluated in Ingenuity Pathway Analysis. In adipose, 509 pathways were predicted to be altered (P<0.01): 202 due to HS, 126 due to ZH, and 181 due to the interaction; these included inflammatory pathways predicted to be upregulated due to HS but downregulated due to the interaction of HS and ZH. In muscle, 113 pathways were predicted to be altered (P<0.01): 23 due to HS, 66 due to ZH, and 24 due to the interaction of HS and ZH. Loci and pathway data in muscle suggest HS induced oxidative stress and that the stress response was moderated by ZH. Metabolic pathways were predicted to be altered due to HS, ZH, and their interaction in both tissues. These data provide evidence that HS and ZH interact to alter expression of genes in metabolic and immune function pathways and that ZH moderates some adverse effects of HS.

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Whole blood transcriptome analysis in feedlot cattle after 35 days of supplementation with a β1-adrenergic agonist

Cited by 4 publications

References 20 publications

IFP35 Is a Relevant Factor in Innate Immunity, Multiple Sclerosis, and Other Chronic Inflammatory Diseases: A Review

IFP35 Is a Relevant Factor in Innate Immunity, Multiple Sclerosis, and Other Chronic Inflammatory Diseases: A Review

MicroRNA-155-5p regulates the Th1/Th2 cytokines expression and the apoptosis of group 2 innate lymphoid cells via targeting TP53INP1 in allergic rhinitis

Transcriptome analyses indicate that heat stress-induced inflammation in white adipose tissue and oxidative stress in skeletal muscle is partially moderated by zilpaterol supplementation in beef cattle

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