Whole body barometric plethysmography: a screening method to investigate airway reactivity and acute lung injuries in freely moving pigs

Halloy, David; Kirschvink, Nathalie; Vincke, Grégoire; Hamoir, Julien; Delvaux, François; Gustin, Pascal

doi:10.1016/j.tvjl.2003.10.015

Cited by 33 publications

(14 citation statements)

References 23 publications

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“…The Penh measured by whole body plethysmography is a functional parameter which has previously shown changes in the expiratory airflow pattern during cholinergic or endotoxin challenges [16]. In this experiment, the increase of Penh recorded on 8 and 14 dpi in group 4, was likely due to airway obstruction and/or to a decrease in the lung elastic recoil induced by the pulmonary inflammatory process as reported in anaesthetised piglets with other infectious pulmonary diseases [21].…”

Section: Discussionsupporting

confidence: 58%

“…A whole body barometric plethysmograph (WBBP) adapted to the size of piglets was built to assess the pulmonary function as previously described by Halloy et al [16]. Briefly, the pig was introduced in the main chamber of the plethysmograph and a differential pressure signal (pressure transducer, Emka Technologies, Paris, France) was measured between this WBBP box and a reference box maintained at atmospheric pressure.…”

Section: Whole Body Barometric Plethysmography: the Penh Measurementmentioning

confidence: 99%

“…In pigs, Penh has been demonstrated to be a screening index allowing to quantify respiratory pattern disorders and is currently used to investigate airway reactivity to pharmacological agents in freely moving pigs but also to predict the amplitude of the pulmonary functional changes occurring during pulmonary diseases especially those of airway resistance [16].…”

Section: Whole Body Barometric Plethysmography: the Penh Measurementmentioning

confidence: 99%

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Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

et al. 2004

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-The aims of this study were (1) to correlate cough and body temperature (BT) with the severity of bronchopneumonia in pigs, (2) to determine whether these clinical signs can be used to early diagnose bronchopneumonia and (3) to assess the predictive values of cough and BT regarding lung lesions. Bronchopneumonia was induced by administering E. coli endotoxin (LPS) combined with Pasteurella multocida type A (PmA) in the trachea of 13 piglets. Saline-instilled negative controls (n = 8), PmA inoculated (n = 6) and LPS instilled (n = 5) groups were also constituted. Cough and BT were recorded daily while the bronchopneumonia severity was assessed using bronchoalveolar lavage fluid (BALF) cytology, cytokines and measurement of lung lesion volume. Changes in expiratory breathing pattern were also measured (Penh). The combination of LPS and PmA induced a subacute bronchopneumonia characterised by macrophage, neutrophil, and lymphocyte infiltration, changes in Penh and an increase in the mRNA level of IFN-γ while IL8, IL-18 and TNF-α mRNA levels remained unchanged. The daily body weight gain of infected animals was significantly reduced. Cough and BT changes were proportional to the intensity of the lung inflammatory process, functional respiratory changes and to the extent of macroscopic lesions. When comparing the individual values of cough and BT to thresholds defined for both parameters, an early diagnosis of pneumonia was possible. Considering the pooled data of each group, it was possible to define thresholds allowing an early segregation between the groups of diseased and healthy piglets. The daily values of cough and BT were predictive for the volume of lung lesions recorded at the end of the trial. In conclusion, cough and BT appear as potential indicators for the intensity and the evolution of the respiratory disease. They also seem to be good predictors for the magnitude of lung lesions and weight gain recorded at the study endpoint.

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Section: Discussionsupporting

confidence: 58%

Section: Whole Body Barometric Plethysmography: the Penh Measurementmentioning

confidence: 99%

Section: Whole Body Barometric Plethysmography: the Penh Measurementmentioning

confidence: 99%

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Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

et al. 2004

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“…Barometric whole-body plethysmography (BWBP) has been validated as a non-invasive pulmonary function test for repeated assessments of the respiratory system in un-sedated, unrestrained and spontaneously breathing cats (Kirschvink et al, 2006). Through calculation of the enhanced pause (Penh), a unitless index of airflow limitation correlated with pulmonary resistance (Hamelmann et al, 1997;Halloy et al, 2004;Kirschvink et al, 2005;Hirt et al, 2008), BWBP has allowed the quantification of both airway obstruction and its reversal with a b 2 -agonist in cats with naturally developing asthma (Rozanski and Hoffman, 1999).…”

Section: Introductionmentioning

confidence: 99%

Functional response to inhaled salbutamol and/or ipratropium bromide in Ascaris suum-sensitised cats with allergen-induced bronchospasms

Leemans

Kirschvink

Clercx

et al. 2010

The Veterinary Journal

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a b s t r a c tKnowledge about the use of inhaled bronchodilators in cats with so-called 'feline asthma' is limited and relies on the experience of clinicians treating these patients. A randomised controlled four-way crossover study was therefore designed to compare the effects of salbutamol (SAL, 100 lg), ipratropium bromide (IB, 20 lg) and a combination of both (SAL/IB, 100 lg/20 lg), delivered through a pressurised metereddose inhaler (pMDI) connected to a spacing chamber, on allergen-induced bronchospasms in five Ascaris suum (AS)-sensitised cats. Four AS bronchial provocation challenges were carried out at 1 week intervals, followed by one of four treatment protocols: SAL, IB, SAL/IB or control (untreated). Enhanced pause (Penh), an estimator of airflow limitation measured by barometric whole-body plethysmography, was repeatedly assessed within 120 min following the administration of each treatment protocol. Responses to inhaled medications were evaluated by calculating the area under the time-response curves (AUC) from 0 to 60 or 120 min after drug administration (AUC 0-60 , AUC 0-120 ), as well as the times required for half-recovery (T 50% ) or for returning to nearly basal conditions (T 20% ).No significant differences were found among the four study groups, with reference to the mean AUC 0-60 , T 20% and T 50% values of Penh (P > 0.05). Mean AUC 0-120 values of Penh were similar between the bronchodilators tested, but were significantly lower than those in the untreated group. It was concluded that inhalation of SAL, IB and SAL/IB via pMDI failed to improve most Penh-derived parameters, which suggested that these bronchodilators were of limited efficacy in reversing allergen-induced bronchospasm in cats. However, further studies using a larger number of animals are warranted to investigate if different drugs or delivery devices or higher dosages may be more effective.

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“…In spite of these limitations, Penh has been shown to be correlated with total lung resistance (RL) in pigs (Halloy et al 2004) and BALBc mice (Hamelmann et al 1997;Adler et al 2004), and has been considered as a surrogate of specific airway resistance (sRaw) in guinea pigs (Bergren 2001;Chong et al 1998;DeLorme and Moss 2002). Thus, the usefulness of barometric plethysmography is still debated.…”

Section: Introductionmentioning

confidence: 99%

Airway responsiveness measured by barometric plethysmography in guinea pigs

et al. 2010

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Barometric plethysmography has become an increasingly used method to indirectly measure respiratory function in unrestrained freely-moving animals. This technique has been criticized because of physiological uncertainty of its major index, the enhanced pause (Penh). Moreover, a recent study raises concerns that during histamine challenges part of the Penh response could be produced by upper airways (nasal) responses. In this study we compared airway responsiveness measured by barometric plethysmography and total lung resistance (RL: ) in guinea pigs, and evaluated the role of upper airways during Penh measurement. Our results showed that intravenous acetylcholine or histamine caused a dose-dependent increase of the Penh values in non-anesthetized guinea pigs, which were correlated with RL: values obtained in separate groups of anesthetized animals. In anesthetized but spontaneously breathing guinea pigs intravenous acetylcholine or histamine also produced a dose-dependent increment of Penh, which was similar regardless if guinea pigs breathed through the nose or through a tracheal tube. Our results suggest that, independently of the physiological meaning of Penh, this index seems to be a useful indirect measurement for evaluating airway responsiveness to intravenous agonists in guinea pigs, and that nasal passage seems not to be involved in this measurement.

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Whole body barometric plethysmography: a screening method to investigate airway reactivity and acute lung injuries in freely moving pigs

Cited by 33 publications

References 23 publications

Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

Pathophysiological changes occurring duringEscherichia coliendotoxin andPasteurella multocidachallenge in piglets: relationship with cough and temperature and predicitive value for intensity of lesions

Functional response to inhaled salbutamol and/or ipratropium bromide in Ascaris suum-sensitised cats with allergen-induced bronchospasms

Airway responsiveness measured by barometric plethysmography in guinea pigs

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