Whole body cortisol and expression of HSP70, IGF-I and MSTN in early development of sea bass subjected to heat shock

Bertotto, Daniela; Poltronieri, Carlo; Negrato, Elena; Richard, Jacopo; Pascoli, Francesco; Simontacchi, Claudia; Radaelli, Giuseppe

doi:10.1016/j.ygcen.2011.08.003

Cited by 48 publications

(39 citation statements)

References 84 publications

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“…Many reports suggested that exposure of fish to common stressors activated the hypothalamicpituitary-interrenal axis leading to increased plasma cortisol (Swift, 1981;Bertotto et al, 2011). However, in our study, the cortisol level did not show significant, which result was the same with the study of Eurasian perch (Douxfils et al, 2012).…”

Section: Discussionsupporting

confidence: 74%

HIFs genes expression and hematology indices responses to different oxygen treatments in an ovoviviparous teleost species Sebastes schlegelii

Wen

et al. 2015

Marine Environmental Research

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Section: Discussionsupporting

confidence: 74%

HIFs genes expression and hematology indices responses to different oxygen treatments in an ovoviviparous teleost species Sebastes schlegelii

Wen

et al. 2015

Marine Environmental Research

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“…Steroid hormones were extracted and whole-embryo cortisol measured by radioimmunoassay (RIA), as described by Bertotto and coworkers 54 . The analyses were performed at the Department of Comparative Biomedicine and Food Science, University of Padova, with anti-cortisol serum (Analytical Antibodies, Bologna).…”

Section: Methodsmentioning

confidence: 99%

nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor

Facchinello

Skobo

Meneghetti

et al. 2017

Sci Rep

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Glucocorticoids (GCs) play important roles in developmental and physiological processes through the transcriptional activity of their cognate receptor (Gr). Using CRISPR/Cas9 technology, we established a zebrafish null Gr mutant line and compared its phenotypes with wild type and a zebrafish line with partially silenced gr (grs357/s357). Homozygous gr−/− larvae are morphologically inconspicuous and, in contrast to GR−/− knockout mice, viable through adulthood, although with reduced fitness and early life survival. Mutants gr−/− are fertile, but their reproductive capabilities fall at around 10 months of age, when, together with cardiac and intestinal abnormalities already visible at earlier stages, increased fat deposits are also observed. Mutants show higher levels of whole-body cortisol associated with overstimulated basal levels of crh and pomca transcripts along the HPI axis, which is unresponsive to a mechanical stressor. Transcriptional activity linked to immune response is also hampered in the gr−/− line: after intestinal damage by dextran sodium sulphate exposure, there are neither inflammatory nor anti-inflammatory cytokine gene responses, substantiating the hypothesis of a dual-action of the GC-GR complex on the immune system. Hence, the zebrafish gr mutant line appears as a useful tool to investigate Gr functions in an integrated in vivo model.

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“…In contrast, heat shock did not impact the expression of myostatin ( MSTN ) mRNA in all groups (Bertotto et al . ). These results suggested that inducible HSP‐70 could be considered as molecular bioindicators of stress in aquaculture.…”

Section: Genes Associated With Environmental Stressorsmentioning

confidence: 97%

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

Eissa

Wang

2014

Reviews in Aquaculture

116

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Stress of aquatic animals occurs due to physical and physiological disturbances in the aquatic environment or system when transportation, crowding, handling or changes of physical and chemical factors take place. There are three regulatory systems having a vital role in stress response: the neural, endocrine and immune systems. Fish exhibit multiple genomic and physiological responses to adjust the compensatory or adaptive mechanism that allows them to mitigate the stressors, maintain their haemostasis and survive. In this review, we describe multiple important genes that are associated with responses to environmental and husbandry stressors and that could be used as biomarkers of environmental and husbandry stressors in fish. The described environmental and husbandry stressors include salinity, temperature, hypoxia and hyperoxia, confinement, density and handling. The main role of stress response in aquatic animals is to compensate or adapt their biological systems and arrange the metabolism to afford the energy required by the stressor and a wide array of metabolic processes and pathways are involved. We summarized and discussed highly significant genes in several organs and tissues that are involved and active during this adaption process. The traditional stress biomarkers in some circumstances have some difficulties in interpretation of results and lead to tricky diagnosis and searching and understanding alternative tools is critical for aquaculture, fisheries and fish welfare. Using genomic tools to study the candidate genes associated with stress responses are often unique signatures or imprints of specific stressors and could determine early signs of stressors.

show abstract

Whole body cortisol and expression of HSP70, IGF-I and MSTN in early development of sea bass subjected to heat shock

Cited by 48 publications

References 84 publications

HIFs genes expression and hematology indices responses to different oxygen treatments in an ovoviviparous teleost species Sebastes schlegelii

HIFs genes expression and hematology indices responses to different oxygen treatments in an ovoviviparous teleost species Sebastes schlegelii

nr3c1 null mutant zebrafish are viable and reveal DNA-binding-independent activities of the glucocorticoid receptor

Transcriptional stress responses to environmental and husbandry stressors in aquaculture species

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