Whole-Body Periodic Acceleration Modifies Experimental Asthma in Sheep

Abraham, William M.; Ahmed, Awad A.; Serebriakov, Irakli; Lauredo, Isabel T.; Bassuk, Jorge; Adams, José A.; Sackner, Marvin A.

doi:10.1164/rccm.200601-048oc

Cited by 15 publications

(8 citation statements)

References 59 publications

(103 reference statements)

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“…Clinicians hypothesize that percussion and shaking loosen adherent mucus and biofilms from the airway surface, making it easier for cough clearance to remove them from the airways. Alternatively, a number of investigators have shown that mechanical stimulation of certain tissue types results in increased epithelial cell release of NO [4]. …”

Section: Introductionmentioning

confidence: 99%

Vest Chest Physiotherapy Airway Clearance is Associated with Nitric Oxide Metabolism

et al. 2013

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Background. Vest chest physiotherapy (VCPT) enhances airway clearance in cystic fibrosis (CF) by an unknown mechanism. Because cilia are sensitive to nitric oxide (NO), we hypothesized that VCPT enhances clearance by changing NO metabolism. Methods. Both normal subjects and stable CF subjects had pre- and post-VCPT airway clearance assessed using nasal saccharin transit time (NSTT) followed by a collection of exhaled breath condensate (EBC) analyzed for NO metabolites (NOx). Results. VCPT shorted NSTT by 35% in normal and stable CF subjects with no difference observed between the groups. EBC NOx concentrations decreased 68% in control subjects after VCPT (before = 115 ± 32 μM versus after = 37 ± 17 μM; P < 0.002). CF subjects had a trend toward lower EBC NOx. Conclusion. We found an association between VCPT-stimulated clearance and exhaled NOx levels in human subjects. We speculate that VCPT stimulates clearance via increased NO metabolism.

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Section: Introductionmentioning

confidence: 99%

Vest Chest Physiotherapy Airway Clearance is Associated with Nitric Oxide Metabolism

et al. 2013

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“…pG z leads to a phosphorylation of eNOS that is correlated with a phosphorylation of Akt in endothelial cells (48). The release of NO into the circulation with pG z has been shown to be physiologically meaningful and long lasting in a sheep model of asthma (1). Additionally, pG z applied to human subjects increases brachial flow-mediated vasodilation and induces release of NO, which is comparable to light to moderate exercise (36,47).…”

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confidence: 99%

Low-amplitude pulses to the circulation through periodic acceleration induces endothelial-dependent vasodilatation

Uryash

Wu²,

Bassuk³

et al. 2009

Journal of Applied Physiology

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Uryash A, Wu H, Bassuk J, Kurlansky P, Sackner MA, Adams JA. Low-amplitude pulses to the circulation through periodic acceleration induces endothelial-dependent vasodilatation. J Appl Physiol 106: 1840 -1847, 2009. First published March 26, 2009 doi:10.1152/japplphysiol.91612.2008.-Low-amplitude pulses to the vasculature increase pulsatile shear stress to the endothelium. This activates endothelial nitric oxide (NO) synthase (eNOS) to promote NO release and endothelial-dependent vasodilatation. Descent of the dicrotic notch on the arterial pulse waveform and a-to-b ratio (a/b; where a is the height of the pulse amplitude and b is the height of the dicrotic notch above the end-diastolic level) reflects vasodilator (increased a/b) and vasoconstrictor effects (decreased a/b) due to NO level change. Periodic acceleration (pG z) (motion of the supine body head to foot on a platform) provides systemic additional pulsatile shear stress. The purpose of this study was to determine whether or not pG z applied to rats produced endothelial-dependent vasodilatation and increased NO production, and whether the latter was regulated by the Akt/phosphatidylinositol 3-kinase (PI3K) pathway. Male rats were anesthetized and instrumented, and pG z was applied. Sodium nitroprusside, N G -nitro-L-arginine methyl ester (L-NAME), and wortmannin (WM; to block Akt/PI3K pathway) were administered to compare changes in a/b and mean aortic pressure. Descent of the dicrotic notch occurred within 2 s of initiating pG z. Dose-dependent increase of a/b and decrease of mean aortic pressure took place with SNP. L-NAME produced a dose-dependent rise in mean aortic pressure and decrease of a/b, which was blunted with pG z. In the presence of WM, pGz did not decrease aortic pressure or increase a/b. WM also abolished the pG z blunting effect on blood pressure and a/b of L-NAME-treated animals. eNOS expression was increased in aortic tissue after pG z. This study indicates that addition of low-amplitude pulses to circulation through pG z produces endothelial-dependent vasodilatation due to increased NO in rats, which is mediated via activation of eNOS, in part, by the Akt/PI3K pathway. vascular resistance; N G -nitro-L-arginine methyl ester, wortmannin; nitric oxide ADDED LOW-AMPLITUDE PULSES to the vasculature were generated with periodic acceleration (pG z ). pG z is produced by a motorized platform that repetitively moves the horizontally oriented body sinusoidally in a head to foot direction. Inertia of fluid as the body accelerates and decelerates adds a small-amplitude pulse to the circulation that is superimposed on the natural pulse, increasing pulsatile shear stress to the endothelium (3, 6, 7). In large-animal models, increased pulsatile shear stress activates endothelial nitric oxide (NO) synthase (eNOS) to release NO into the circulation, which, in turn, induces endothelial-dependent pulmonary and systemic vasodilatation, as well as increasing organ blood flows (3,5,6). pG z applied to anesthetized swine increases serum nitrite, which is ...

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“…N-Nitro-L-arginine methyl ester hydrochloride (L-NAME; Sigma-Aldrich, St. Louis, MO) was given as an intravenous injection; the dose per animal was 25 mg/kg in 20 ml of 0.9% NaCl as previously described. [19]…”

Section: 0 Methodsmentioning

confidence: 99%

“…[16–18] In sheep, this technology modulated antigen induced inflammatory responses such as inhibiting nuclear factor kappa beta activity. [19] The purpose of the current investigation was to determine whether NO delivered via the blood stream could access airway epithelium to increase TMV and provide protection against human neutrophil elastase (HNE) induced slowed MCC. [20, 21]…”

Section: 0 Introductionmentioning

confidence: 99%

Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep

et al. 2019

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The purpose of this investigation was to ascertain whether nitric oxide (NO) released into the circulation by a noninvasive technology called whole body periodic acceleration (WBPA) could increase mucociliary clearance (MCC). It was based on observations by others that nitric oxide donor drugs increase ciliary beat frequency of nasal epithelium without increasing mucociliary clearance. Tracheal mucous velocity (TMV), a reflection of MCC, was measured in sheep after 1-hour treatment of WBPA and repeated after pretreatment with the NO synthase inhibitor, L-NAME to demonstrated action of NO. Aerosolized human neutrophil elastase (HNE) was administered to sheep to suppress TMV as might occur in cystic fibrosis and other inflammatory lung diseases. WBPA increased TMV to a peak of 136% of baseline 1h after intervention, an effect blocked by L-NAME. HNE reduced TMV to 55% of baseline but slowing was reversed by WBPA, protection lost in the presence of L-NAME. NO released into the circulation from eNOS by WBPA can acutely access airway epithelium for improving MCC slowed in cystic fibrosis and other inflammatory lung diseases as a means of enhancing host defense against pathogens.

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Whole-Body Periodic Acceleration Modifies Experimental Asthma in Sheep

Abstract: Whole-body pGz modulates allergen-induced airway responses in allergic sheep.

Cited by 15 publications

References 59 publications

Vest Chest Physiotherapy Airway Clearance is Associated with Nitric Oxide Metabolism

Vest Chest Physiotherapy Airway Clearance is Associated with Nitric Oxide Metabolism

Low-amplitude pulses to the circulation through periodic acceleration induces endothelial-dependent vasodilatation

Whole body periodic acceleration in normal and reduced mucociliary clearance of conscious sheep

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