Whole-Cell Biophysical Parameters Measured with Multimodal Quantitative-Phase Digital Holographic Microscopy and Flow Assays

Abstract

We demonstrate the measurement of whole-cell biophysical parameters with quantitative-phase digital holographic microscopy and flow assays. For different cell types, whole-cell mechanical properties and refractive index, mean cell thickness and absolute cell volume were obtained.

Characterization of Optical, Thermal, and Viscoelastic Properties of Pollenkitt in Angiosperm Pollen Using In-Line Digital Holographic Microscopy

“…37 As a result, DHM has been extensively employed to study the biophysical properties of colloidal objects, for example, by establishing the relationship between the cell mechanism and biological function by measuring variations in the quantitative phase signal. 41 DHM has previously been used for tasks such as cell counting, recognition, classification, 42 monitoring cellular dry mass, 43 analyzing cell membrane fluctuations, 44 and reconstructing the intracellular 3D RI distribution through tomographic approaches. 45 Essential biophysical cell parameters, 46 including shape, morphology, intracellular RI, and water permeability, have been successfully calculated from the quantitative phase signal.…”

“…This hologram encodes phase information, allowing for the extraction of crucial details about the microscopic object, including its 3D structure and composition . As a result, DHM has been extensively employed to study the biophysical properties of colloidal objects, for example, by establishing the relationship between the cell mechanism and biological function by measuring variations in the quantitative phase signal . DHM has previously been used for tasks such as cell counting, recognition, classification, monitoring cellular dry mass, analyzing cell membrane fluctuations, and reconstructing the intracellular 3D RI distribution through tomographic approaches .…”