Whole cell imprinting based Escherichia coli sensors: A study for SPR and QCM

Molecularly-imprinted polymer sensors: realising their potential

“…Despite these limitations, they showed that thermo-imprinted nano-films could be successfully implemented in a QCM assay as the recognition element (Buchegger et al, 2014). Yilmaz et al (2015) developed a rapid and selective detection method via micro-contact imprinting of whole E. coli cells on the two classic, label-free sensing platforms, optical (SPR) and mass-sensitive (QCM), and compared their performances. They used a polymerisable form of amino acid (L-histidine) to create recognition layers similar to natural antibodies while gaining chemical and physical stability to enhance reusability and reduce production cost (Yilmaz et al, 2015).…”

“…Yilmaz et al (2015) developed a rapid and selective detection method via micro-contact imprinting of whole E. coli cells on the two classic, label-free sensing platforms, optical (SPR) and mass-sensitive (QCM), and compared their performances. They used a polymerisable form of amino acid (L-histidine) to create recognition layers similar to natural antibodies while gaining chemical and physical stability to enhance reusability and reduce production cost (Yilmaz et al, 2015). Such comparative studies have the potential to resolve important practical issue such as the choice of the best instrument for a particular application.…”

Label free Affinity sensing: application to food analysis

“…Amino acid based recognition element, Nmethacryloyl-L-histidine methylester (a polymerizable form of histidine) was used for MIP synthesis [71]. Response times (time required to reach 95 % of steady state) were calculated as 113 s for SPR and 56 s for QCM so both systems are very rapid compared to the classical bacterial culture methods which may take up to 7-8 days for the answer.…”

The Development of a Portable SPR Bioanalyzer for Sensitive Detection of Escherichia coli O157:H7

“…With the advantages of label-free, non-intrusive, real-time biomolecule detection, SPR biosensors have been widely applied in life science, drug development, environmental monitoring, and food safety [17,18,19]. An increasing number of research groups have been dedicated to studying the detection of the pathogens, such as Staphylococcus aureus , bacteriophages, and E. coli O157:H7 using quartz crystal microbalance (QCM) and the electrochemical method [20,21,22,23,24]. Compared with the QCM and the electrochemical method, SPR has some prominent advantages: firstly, it can achieve real time monitoring of biomolecular interactions; secondly, it can determine both kinetics parameters.…”