Whole genome sequence analysis of Mycobacterium bovis bacillus Calmette–Guérin (BCG) Tokyo 172: A comparative study of BCG vaccine substrains

Seki, Masaaki; Honda, Ikuro; Fujita, Isao; Yano, Ikuya; Yamamoto, Saburo; Koyama, Akira

doi:10.1016/j.vaccine.2009.01.034

Cited by 68 publications

(57 citation statements)

References 36 publications

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“…These genomes included three MTC members, i.e. M. tuberculosis H37Rv (AL123456) (Cole et al, 1998), M. bovis (BX248333) (Garnier et al, 2003) and M. bovis BCG (Pasteur and Tokyo strains) (NC_008769, AP010918) (Brosch et al, 2007;Seki et al, 2009), two MAC members, i.e. M. avium 104 (CP00479) and M. avium subsp.…”

Section: Methodsmentioning

confidence: 99%

Paradoxical conservation of a set of three cellulose-targeting genes in Mycobacterium tuberculosis complex organisms

et al. 2010

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The genome of the tuberculosis agent Mycobacterium tuberculosis encodes a putative cellulosebinding protein (CBD2), one candidate cellulase (Cel12), and one fully active cellulase (Cel6). This observation is puzzling, because cellulose is a major component of plant cell walls, whereas M. tuberculosis is a human pathogen without known contact with plants. In order to investigate the biological role of such cellulose-targeting genes in M. tuberculosis we report here the search for and transcription analysis of this set of genes in the genus Mycobacterium. An in silico search for cellulose-targeting orthologues found that only 2.5 % of the sequenced bacterial genomes encode the Cel6, Cel12 and CBD2 gene set simultaneously, including those of the M. tuberculosis complex (MTC) members. PCR amplification and sequencing further demonstrated the presence of these three genes in five non-sequenced MTC bacteria. Among mycobacteria, the combination of Cel6, Cel12 and CBD2 was unique to MTC members, with the exception of Mycobacterium bovis BCG Pasteur, which lacked CBD2. RT-PCR in M. tuberculosis H37Rv indicated that the three cellulose-targeting genes were transcribed into mRNA. The present work shows that MTC organisms are the sole mycobacteria among very few organisms to encode the three cellulose-targeting genes CBD2, Cel6 and Cel12. Our data point toward a unique, yet unknown, relationship with non-plant cellulose-producing hosts such as amoebae.

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Section: Methodsmentioning

confidence: 99%

Paradoxical conservation of a set of three cellulose-targeting genes in Mycobacterium tuberculosis complex organisms

et al. 2010

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“…BCG has lost its virulence at the price of the deletion of five DNA regions called regions of difference (RDs) covering 38 ORFs (Seki et al, 2009), of which region of difference 1 (RD1) is the most prominent. Some previous reports have demonstrated that RD1 protein ESAT-6 promotes differentiation of Th17 cells, which contributes to improved protection against M.tb challenge (Chatterjee et al, 2011).…”

Section: Bovis Has a Stronger Ability Than Bcg To Induce Proinflammentioning

confidence: 99%

“…It is an attenuated strain of M. bovis developed by Calmette and Guérin which lost its virulence after 230 passages over a period of 13 years and has a deletion of five DNA regions including 38 ORFs (Seki et al, 2009). Although the efficacy of BCG in preventing active TB in adults has been challenged, it saves millions of lives by preventing severe, disseminated extra-pulmonary TB, including miliary and meningitis TB in young children (Parthasarathy, 2003).…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

Zhang

Luo

et al. 2013

Microbiology

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Mycobacterium tuberculosis can infect dendritic cells (DCs), but the molecular mechanism by which these cells contribute to tuberculosis pathogenesis is largely unclear. Using Mycobacterium bovis and the attenuated strain M. bovis BCG as model strains, we analysed cytokine and chemokine secretion in murine DCs infected with M. bovis and BCG at 6, 12 and 24 h post-infection. BCG enhanced production of MCP-1, RANTES, IL-12, TNF-a and IL-6 in DCs, while M. bovis promoted secretion of IL-1b, IL-10 and IL-23. Heat-killed BCG and M. bovis both stimulated cytokine production, but at significantly lower concentrations than corresponding live bacteria. Quantitative RT-PCR and Western blotting indicated that NF-kB regulates production of most cytokines and chemokines. After DCs were infected for 24 h, the culture was used to activate naïve CD4 + T cells. A combination of the supernatant and activated DCs infected with M. bovis gave high expression of foxp3 and IL-10, directing differentiation of naïve CD4 + T cells into regulatory T cells (CD4 + CD25 + Foxp3 + ) more effectively than BCG. Furthermore, M. bovis-infected DC cultures induced CD4 + T cells to express significantly higher levels of IL-17, a Th17-type cytokine, while BCG-infected DC cultures stimulated an apparently higher production of IFN-c, a Th1-type cytokine. In addition, the mycobacteria did not exert a direct effect on the differentiation of CD4 + T cells. These differential cytokine profiles in DCs and CD4 + T cells, and the resultant development of CD4 + T subsets, may be related to the pathogenesis of tuberculosis.

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“…The complete genomes of strains Pasteur (4) and Tokyo (18) have been reported, as well as draft sequences for strains China, Denmark, Russia, and Tice (16). Here we report the complete, annotated genome sequence of M. bovis BCG Moreau.…”

mentioning

confidence: 99%

Genome Sequence of Mycobacterium bovis BCG Moreau, the Brazilian Vaccine Strain against Tuberculosis

et al. 2011

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Whole genome sequence analysis of Mycobacterium bovis bacillus Calmette–Guérin (BCG) Tokyo 172: A comparative study of BCG vaccine substrains

Cited by 68 publications

References 36 publications

Paradoxical conservation of a set of three cellulose-targeting genes in Mycobacterium tuberculosis complex organisms

Paradoxical conservation of a set of three cellulose-targeting genes in Mycobacterium tuberculosis complex organisms

Mycobacterium bovis and BCG induce different patterns of cytokine and chemokine production in dendritic cells and differentiation patterns in CD4+ T cells

Genome Sequence of Mycobacterium bovis BCG Moreau, the Brazilian Vaccine Strain against Tuberculosis

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